ID   CTU2_DROVI              Reviewed;         400 AA.
AC   B4M8J8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GJ18095;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15010-1051.87;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH940654; EDW57524.1; -; Genomic_DNA.
DR   RefSeq; XP_002057451.1; XM_002057415.2.
DR   AlphaFoldDB; B4M8J8; -.
DR   SMR; B4M8J8; -.
DR   STRING; 7244.FBpp0232512; -.
DR   EnsemblMetazoa; FBtr0234020; FBpp0232512; FBgn0205260.
DR   GeneID; 6634299; -.
DR   KEGG; dvi:6634299; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   InParanoid; B4M8J8; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B4M8J8; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008792; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..400
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369278"
SQ   SEQUENCE   400 AA;  43888 MW;  2C57AD97C9F03382 CRC64;
     MCSIGEDDFG DEGATHAMLP GSSTVGTVIT AGSCNKCGLN SLELYKLNFR AAECHQCFLS
     YARHKFRAAL GAAKALPRNA EVLLLVDGSA ESLVLLDMLH FAQTQNTFKR LHCSARVLHI
     DAQSSPSEND PLPMLNELRE RYAPFEFYVI QLGAEVHSLK LLKDYSASRL DSTDNKLRSL
     TARQDYVRQQ RKRLIGAVAL QLQCTHVFEP SISSSLAAQL LTSVALGRGG SVALDVALLD
     DRLQGAVTLL RPLKDLNEQE VQFYIKAQQL KPFRSDESSL LSDASLQNLT SAFVANLQLN
     YASTVSTVFR TGDKIAAKRQ PIEEGPSTCA LCQSDLDSGL SDTLLAIEYS RAVSEMGVAL
     QQQNNMEALE QRARERLNSM DNLCHACRNI HAELTHGSLI