ID   CTU2_DROYA              Reviewed;         404 AA.
AC   B4PAZ6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GE12647;
OS   Drosophila yakuba (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tai18E2 / Tucson 14021-0261.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CM000158; EDW90425.1; -; Genomic_DNA.
DR   RefSeq; XP_002090713.1; XM_002090677.2.
DR   AlphaFoldDB; B4PAZ6; -.
DR   SMR; B4PAZ6; -.
DR   STRING; 7245.FBpp0257657; -.
DR   EnsemblMetazoa; FBtr0259165; FBpp0257657; FBgn0230375.
DR   GeneID; 6529720; -.
DR   KEGG; dya:Dyak_GE12647; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B4PAZ6; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000002282; Chromosome 2R.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..404
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369280"
SQ   SEQUENCE   404 AA;  44750 MW;  CE4185C04D791A34 CRC64;
     MCSIGEDDFG DEGAAHAMVV EALPLGIVLS PGNCSKCDVN SNELYKLNFR EAECRECFLA
     YARHKFRAAL GAAKILPRNA EVLLVLDGSA KSLVLLDMLH FAQTQNTFKR LHCNVHVVYV
     EEQHVQDRDP VGLEDLLSVS RQYAPFEFYV IELGQGCSLQ RIKDYSPSLK ANNELIYKLQ
     KLQSLTARQD YLQQHRKNLI CSVAQSLHCT HVFESNISVD LATQLLTAIA LGRGASAALD
     VALLDDRLSG DVKLLRPLKD LSEQEITFYI HAQRLKPLFQ NGSHYGMERG QTASIQNLTS
     AFVANLQQNY ASTVSTVFRT GDKIAVNSNP EQASCVHCRS ALDSELSDTL LAIEYSRSVS
     EAGVSLNKNE KDLEGLAKKR LEKQDGLCHA CRTIQAEFDS GNLL