ID   CTU2_ORYSI              Reviewed;         464 AA.
AC   A2ZM73;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   Name=CTU2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   Synonyms=NCS2 {ECO:0000255|HAMAP-Rule:MF_03054}; ORFNames=OsI_38929;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CM000137; EAY83707.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2ZM73; -.
DR   SMR; A2ZM73; -.
DR   STRING; 39946.A2ZM73; -.
DR   PRIDE; A2ZM73; -.
DR   EnsemblPlants; BGIOSGA035918-TA; BGIOSGA035918-PA; BGIOSGA035918.
DR   Gramene; BGIOSGA035918-TA; BGIOSGA035918-PA; BGIOSGA035918.
DR   HOGENOM; CLU_048050_0_0_1; -.
DR   OMA; YVRHKFR; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000007015; Chromosome 12.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..464
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369283"
SQ   SEQUENCE   464 AA;  50396 MW;  3B85139E8B3C6660 CRC64;
     MAAAAASSCG GAGCGPHCSS SASAAAVEDA AAAAAEKVGR LSLSRECGKC GGGAAAVAVA
     GGLGLCGECF RANLLGKFKL AVTSNAMVRP TDSVLLAFSG GPASRVALQF IHEMRCKAIE
     SWDVSNSQAL PVFGVGVAFV DESVLCSKPR DEIEMAIEDI RSIVSSLSTG VKAMHIVRLE
     DVFSTESEDG ERRLREAVDM IGDDTGREDF LRCLRMLSLQ KIAMENGYAK IMLGSCASAI
     ACHVLSATVK GQGYSLPADV QYVDTRWEIP VVLPLRDCLA QELTLLCELD SLKTQQHLDR
     PSNGINSLVA SFIKRLREEN PSREHTIVRT AQKLKPFSFN KFSADGYHDF LPSRLRPKFQ
     KVDSDESTFS EILCLMCGSP FSESELQNLE STKHKAQKKI DLYTAHCCQS CYFQILPAGE
     NLNEHFFSLL PKLWTGKMDT ISDSHSLLRD QIEEYLLEEN DDGN