ID   CTX23_LACTA             Reviewed;          70 AA.
AC   C0HJV4;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2016, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Cytoinsectotoxin-2c {ECO:0000303|PubMed:27287558};
DE            Short=CIT-2c {ECO:0000303|PubMed:27287558};
OS   Lachesana tarabaevi (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC   Lachesana.
OX   NCBI_TaxID=379576 {ECO:0000303|PubMed:27287558};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   TOXIC DOSE.
RC   TISSUE=Venom {ECO:0000303|PubMed:27287558};
RX   PubMed=27287558; DOI=10.1042/bcj20160436;
RA   Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA   Vassilevski A.A.;
RT   "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL   Biochem. J. 473:2495-2506(2016).
CC   -!- FUNCTION: Insecticidal and antimicrobial peptide. Has insecticidal
CC       activity against larvae of flesh fly S.carnaria. Has antibacterial
CC       activity against Gram-positive bacterium B.subtilis B-501 (MIC=1.25 uM)
CC       and Gram-negative bacterium E.coli DH5alpha (MIC=2.5 uM).
CC       {ECO:0000269|PubMed:27287558}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27287558}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:27287558}.
CC   -!- DOMAIN: Both the N-terminus (1-33) and the C-terminus (38-70) of the
CC       mature peptide form alpha-helices which probably disrupt target cell
CC       membranes. The linker region (34-37) probably derives from a processing
CC       quadruplet motif (PQM), found in propeptides of many zodatoxins,
CC       hinting at a fusion of two originally separate membrane-active
CC       peptides. {ECO:0000250|UniProtKB:P85253}.
CC   -!- MASS SPECTROMETRY: Mass=8198.8; Mass_error=0.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27287558};
CC   -!- TOXIC DOSE: LD(50) is 40 ug/g in larvae of flesh fly S.carnaria.
CC       {ECO:0000269|PubMed:27287558}.
CC   -!- SIMILARITY: Belongs to the cationic peptide 06 (cytoinsectotoxin)
CC       family. {ECO:0000305}.
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DR   AlphaFoldDB; C0HJV4; -.
DR   SMR; C0HJV4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Secreted; Toxin.
FT   PEPTIDE         1..70
FT                   /note="Cytoinsectotoxin-2c"
FT                   /evidence="ECO:0000269|PubMed:27287558"
FT                   /id="PRO_0000437250"
SQ   SEQUENCE   70 AA;  8198 MW;  28E28EC4F73FBE49 CRC64;
     SWDSIWKSAK NKMDKIMRQK VAKWMAKKEG KSVEEVQAKV DAMSKKDIRM HVISHYGKKA
     FEQLSKSLEE