ID   CTX_PSEAI               Reviewed;         286 AA.
AC   P14608;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Cytotoxin;
DE   AltName: Full=Leucocidin;
DE   Flags: Precursor;
GN   Name=ctx;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=PA158;
RX   PubMed=2507866; DOI=10.1111/j.1365-2958.1989.tb00235.x;
RA   Hayashi T., Kamio Y., Hishinuma F., Usami Y., Titani K., Terawaki Y.;
RT   "Pseudomonas aeruginosa cytotoxin: the nucleotide sequence of the gene and
RT   the mechanism of activation of the protoxin.";
RL   Mol. Microbiol. 3:861-868(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=1695085; DOI=10.1007/bf00245265;
RA   Orlik-Eisel G., Lutz F., Henschen A., Eisel U., Struckmeier M.,
RA   Kraeuter J., Niemann H.;
RT   "The cytotoxin of Pseudomonas aeruginosa: cytotoxicity requires proteolytic
RT   activation.";
RL   Arch. Microbiol. 153:561-568(1990).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-21.
RC   STRAIN=PA158;
RX   PubMed=2497292; DOI=10.1016/0882-4010(89)90013-2;
RA   Hayashi T., Kamio Y., Terawaki Y.;
RT   "Purification and characterization of cytotoxin from the crude extract of
RT   Pseudomonas aeruginosa.";
RL   Microb. Pathog. 6:103-112(1989).
CC   -!- FUNCTION: Cytotoxin is thought to form hydrophilic pores in cell
CC       membranes.
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Produced as a cell-associated
CC       inactive precursor which is not secreted extracellularly. The toxin is
CC       converted into its active form by the action of proteases concomitantly
CC       with cell lysis and is released into the medium.
CC   -!- MISCELLANEOUS: This protein toxin may be one of the virulence factors
CC       because it shows strong cytotoxic effects on most eukaryotic cells,
CC       especially leukocytes.
CC   -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR   EMBL; X14956; CAA33079.1; -; Genomic_DNA.
DR   PIR; A60690; A60690.
DR   RefSeq; WP_015967180.1; NZ_RXBS01000006.1.
DR   AlphaFoldDB; P14608; -.
DR   SMR; P14608; -.
DR   TCDB; 1.C.13.1.1; the channel-forming leukocidin cytotoxin (ctx) family.
DR   PATRIC; fig|287.2551.peg.4090; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004991; Aerolysin-like.
DR   Pfam; PF03318; ETX_MTX2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Toxin; Virulence.
FT   CHAIN           1..266
FT                   /note="Cytotoxin"
FT                   /id="PRO_0000035634"
FT   PROPEP          267..286
FT                   /id="PRO_0000035635"
SQ   SEQUENCE   286 AA;  31681 MW;  528ECFE4F0626444 CRC64;
     MNDIDTITNA WGRWKTAQYG TTCWFTESTQ YGRNKDTRSY MQHQTNVSAP KDLVYSNFVQ
     QDGGSTLLGQ YDMINEGSQV IELAVNLQQG LVDTFTWSVT EQLKVGVEVK VKANIPLVGG
     AEITSTVELS LSSTQGASTS KSSNYGASTK VLISPHSHGW GEVALSFTEL RTQWVGNVGL
     QGYVAIWFNN KVALNNDGDY HYLWFIPVEQ VFWECVQHNI VNTSGYVVQG NGVLAQATGT
     FHSSVGLNLK TIAHERPYPE TSEAVRTFYN YASLVPDLET RVRSAE