ID   CTX_SEPES               Reviewed;        1052 AA.
AC   B2DCR8;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=SE-cephalotoxin;
DE            Short=SE-ctx;
DE   Flags: Precursor;
OS   Sepia esculenta (Golden cuttlefish) (Diphtherosepion dabryi).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda;
OC   Coleoidea; Decapodiformes; Sepiida; Sepiina; Sepiidae; Sepia.
OX   NCBI_TaxID=31210;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-52; 941-947; 334-345 AND
RP   347-381, SUBUNIT, GLYCOSYLATION AT ASN-41 AND ASN-353, AND TOXIC DOSE.
RC   TISSUE=Posterior salivary gland;
RX   PubMed=18694775; DOI=10.1016/j.toxicon.2008.07.007;
RA   Ueda A., Nagai H., Ishida M., Nagashima Y., Shiomi K.;
RT   "Purification and molecular cloning of SE-cephalotoxin, a novel
RT   proteinaceous toxin from the posterior salivary gland of cuttlefish Sepia
RT   esculenta.";
RL   Toxicon 52:574-581(2008).
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18694775}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary gland.
CC   -!- TOXIC DOSE: LD(50) is 2 ug/kg against crabs.
CC       {ECO:0000269|PubMed:18694775}.
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DR   EMBL; AB363989; BAG24412.1; -; mRNA.
DR   AlphaFoldDB; B2DCR8; -.
DR   iPTMnet; B2DCR8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00112; LDLa; 1.
DR   Gene3D; 2.20.100.10; -; 1.
DR   Gene3D; 4.10.400.10; -; 1.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR036055; LDL_receptor-like_sf.
DR   InterPro; IPR023415; LDLR_class-A_CS.
DR   InterPro; IPR002172; LDrepeatLR_classA_rpt.
DR   InterPro; IPR039051; SE-CTX-like.
DR   InterPro; IPR000436; Sushi_SCR_CCP_dom.
DR   InterPro; IPR000884; TSP1_rpt.
DR   InterPro; IPR036383; TSP1_rpt_sf.
DR   PANTHER; PTHR40472; PTHR40472; 1.
DR   Pfam; PF00057; Ldl_recept_a; 1.
DR   Pfam; PF00090; TSP_1; 1.
DR   SMART; SM00192; LDLa; 1.
DR   SMART; SM00209; TSP1; 1.
DR   SUPFAM; SSF57424; SSF57424; 1.
DR   SUPFAM; SSF82895; SSF82895; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS50026; EGF_3; 1.
DR   PROSITE; PS01209; LDLRA_1; 1.
DR   PROSITE; PS50068; LDLRA_2; 1.
DR   PROSITE; PS50923; SUSHI; 1.
DR   PROSITE; PS50092; TSP1; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Disulfide bond; EGF-like domain;
KW   Glycoprotein; Secreted; Signal; Sushi; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..29
FT                   /evidence="ECO:0000269|PubMed:18694775"
FT                   /id="PRO_0000359437"
FT   CHAIN           30..1052
FT                   /note="SE-cephalotoxin"
FT                   /id="PRO_0000359438"
FT   DOMAIN          460..497
FT                   /note="EGF-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DOMAIN          709..769
FT                   /note="Sushi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT   DOMAIN          768..821
FT                   /note="TSP type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT   DOMAIN          819..859
FT                   /note="LDL-receptor class A"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00124"
FT   COILED          130..194
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        41
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:18694775"
FT   CARBOHYD        353
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:18694775"
FT   CARBOHYD        576
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        715
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        786
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        464..474
FT                   /evidence="ECO:0000250"
FT   DISULFID        468..485
FT                   /evidence="ECO:0000250"
FT   DISULFID        487..496
FT                   /evidence="ECO:0000250"
FT   DISULFID        711..752
FT                   /evidence="ECO:0000250"
FT   DISULFID        739..767
FT                   /evidence="ECO:0000250"
FT   DISULFID        780..814
FT                   /evidence="ECO:0000250"
FT   DISULFID        784..820
FT                   /evidence="ECO:0000250"
FT   DISULFID        795..804
FT                   /evidence="ECO:0000250"
FT   DISULFID        829..847
FT                   /evidence="ECO:0000250"
FT   DISULFID        841..858
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1052 AA;  120495 MW;  86E4400BF7EA24D5 CRC64;
     MMGTSRCVIL LFALLLWAAN AAPPEIHTTR PNVPEEIKRP NSTEIETPAV KQLETPSIFL
     LTTLEVAEAD VDSTLETMKD RNKKNSAKLS KIGNNMKSLL SVFSVFGGFL SLLSVVTTTS
     DLQVISDMFT GVNRKLDQIN DKLDKLDNSV ELQGLLTNYI PWQYSVKNGI EKLIETYKKM
     VEETDMNKRR LMAENFILFF ENNQIESNIN NLIKLTTTTD AVHQNMLFNE LLDEAGCDII
     RLTRIYMHVR RIFYQGTQLV LAYNSFKQMD PPEMKKYLNA LIFIRNMYQS RVWHCKETTI
     AQSKKDIKDI VKTNAKFGIT TVLRKINSEL SRKYPWYSWS IVTVKKMLAN QRNSTLGNQF
     YEMEAVGPHG SNFVVIWQGF KEHSQCEDIQ KANTIAVLTI CKSCHQSHVF TPSNMLNKNT
     CPNNQYPQVK AFIDRREPFR DEIQRKKSDV FWVAAGFKAP GNPCNHGCNG HGECKVVPYT
     DQFQCFCHGN YEGKMCQKKI QMKRDISKLI SDLQTGYKNA FNVPSLTNIL IQGENLAKQL
     KKMIQRIDNQ FELTHILVKY ISDLQKLDYI LKISFNYSKK KITVDAFSRR MKAFLSLNPV
     DFIFQQLSNA ILAEGFTDIQ GKDFFNTFKR MIASNRDACT APYGNEATIL LERLSRLDLT
     AAESILAYYS FESNYLNPAN MKRMLENAKQ LVRDSKRRMR SYARYWERTS CPPLNVTHLT
     QTGCGALLSF EGMKVKLSCD GGRAAVPQNI ECVNVNGNLQ WSATPKCESS WSRWSKWSAC
     ASTCGNATQS RRRRCLGQSE SEKCIGPSKQ VRKCFVEDCC QEKYGKFKCD NNKCISLSRV
     CDGNDDCRNA EDESKSRCKY LRSGDRIALR NMAYSQEWLS VQYTDAVQAD LYYGRAYLNH
     CIKGDHVTSS EWNSCAGQSL LIYGNYENGK IGKAIRFGDK IAMYYRKTNY HYRWFICYPT
     YCMTYTCPKK AGSFTFGPNG GCDEYEFYII NYNDKLSRDP VKPGDVITLA NNRGSVKGNG
     YNRNININDC TVKRAQDDRI ECNANAWQIF IK