ID   CU20_MANSE              Reviewed;         200 AA.
AC   Q8T635;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Pupal cuticle protein 20;
DE   AltName: Full=MS-PCP20;
DE            Short=MsCP20;
DE   Flags: Precursor;
GN   Name=PCP20;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130 {ECO:0000312|EMBL:AAL90881.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-200, FUNCTION,
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MASS SPECTROMETRY.
RC   TISSUE=Pharate pupal cuticle {ECO:0000269|PubMed:12609518};
RX   PubMed=12609518; DOI=10.1016/s0965-1748(02)00247-3;
RA   Suderman R.J., Andersen S.O., Hopkins T.L., Kanost M.R., Kramer K.J.;
RT   "Characterization and cDNA cloning of three major proteins from pharate
RT   pupal cuticle of Manduca sexta.";
RL   Insect Biochem. Mol. Biol. 33:331-343(2003).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 19-40, AND SUBCELLULAR LOCATION.
RC   TISSUE=Pharate pupal cuticle {ECO:0000269|PubMed:10646967};
RX   PubMed=10646967; DOI=10.1016/s0965-1748(99)00091-0;
RA   Hopkins T.L., Krchma L.J., Ahmad S.A., Kramer K.J.;
RT   "Pupal cuticle proteins of Manduca sexta: characterization and profiles
RT   during sclerotization.";
RL   Insect Biochem. Mol. Biol. 30:19-27(2000).
CC   -!- FUNCTION: Component of the pupal abdominal endocuticle. May have
CC       important role in the larval cuticle structure.
CC       {ECO:0000269|PubMed:12609518, ECO:0000303|PubMed:10646967}.
CC   -!- SUBCELLULAR LOCATION: Note=Synthesized in the epidermis during the
CC       larval-pupal transformation and then secreted into the pharate pupal
CC       cuticle. {ECO:0000269|PubMed:10646967, ECO:0000269|PubMed:12609518}.
CC   -!- DEVELOPMENTAL STAGE: Present at low levels early in the fifth larval
CC       stadium, followed by a large increase in abundance prior to pupal
CC       ecdysis. {ECO:0000269|PubMed:12609518}.
CC   -!- MASS SPECTROMETRY: Mass=17894; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12609518};
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DR   EMBL; AF487520; AAL90881.1; -; mRNA.
DR   AlphaFoldDB; Q8T635; -.
DR   GO; GO:0042302; F:structural constituent of cuticle; IEA:UniProtKB-KW.
DR   InterPro; IPR031311; CHIT_BIND_RR_consensus.
DR   InterPro; IPR000618; Insect_cuticle.
DR   Pfam; PF00379; Chitin_bind_4; 1.
DR   PRINTS; PR00947; CUTICLE.
DR   PROSITE; PS00233; CHIT_BIND_RR_1; 1.
DR   PROSITE; PS51155; CHIT_BIND_RR_2; 1.
PE   1: Evidence at protein level;
KW   Cuticle; Direct protein sequencing; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:10646967,
FT                   ECO:0000269|PubMed:12609518"
FT   CHAIN           19..200
FT                   /note="Pupal cuticle protein 20"
FT                   /evidence="ECO:0000269|PubMed:12609518"
FT                   /id="PRO_0000006410"
FT   DOMAIN          93..162
FT                   /note="Chitin-binding type R&R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT   REGION          100..119
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          177..200
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        154
FT                   /note="L -> V (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        165
FT                   /note="P -> I (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   200 AA;  19481 MW;  FE8B0A54E41414E8 CRC64;
     MATILLVLTA VLIAVGSCGR LEPQYLPPRP GNGFGGGAGS GGNAGLGGGA GGSGAGAGGF
     GGSGAGAYGG SGGSHGGGAQ IPITSFENVN NGDGSYHFSY ETGNGISAQE SGAPRAPGPE
     GLAVTAEGAF SYRTPDGQQI ALTYTADENG FHPLGPHLPT PPPIPEEILR SIEFNRQNPS
     SEGSYNGGNG GSGGSNGYHY