ID   CU317_LATCU             Reviewed;          47 AA.
AC   C0HK82;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   29-SEP-2021, entry version 7.
DE   RecName: Full=Bacteriocin curvaticin DN317 {ECO:0000303|PubMed:27812926};
OS   Latilactobacillus curvatus (Lactobacillus curvatus).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=28038 {ECO:0000303|PubMed:27812926};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP   LOCATION, AND MASS SPECTROMETRY.
RC   STRAIN=DN317 {ECO:0000303|PubMed:27812926};
RX   PubMed=27812926; DOI=10.1007/s12602-016-9237-7;
RA   Zommiti M., Almohammed H., Ferchichi M.;
RT   "Purification and Characterization of a Novel Anti-Campylobacter
RT   Bacteriocin Produced by Lactobacillus curvatus DN317.";
RL   Probiotics Antimicrob. Proteins 8:191-201(2016).
CC   -!- FUNCTION: Has bactericidal activity against various Gram-negative
CC       Campylobacter, and the Gram-positive L.monocytogenes and B.subtilis. In
CC       vitro, inhibits C.jejuni strain ATCC 33560 (MIC=27.3 ug/ml).
CC       {ECO:0000269|PubMed:27812926}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Stable between pH 4.0 and pH 8.0. {ECO:0000269|PubMed:27812926};
CC       Temperature dependence:
CC         Thermostable. Retains 100% activity after incubation at 60 degrees
CC         Celsius for 60 minutes and 15% after incubation at 121 degrees
CC         Celsius for 15 minutes. {ECO:0000269|PubMed:27812926};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27812926}.
CC   -!- MASS SPECTROMETRY: Mass=4448.09; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27812926};
CC   -!- MISCELLANEOUS: Antimicrobial activity is lost upon treatment with
CC       proteinase K, pronase, pepsin, trypsin, chymotrypsin and papain, but
CC       not when treated with catalase, lysozyme or lipase.
CC       {ECO:0000269|PubMed:27812926}.
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Secreted.
FT   CHAIN           1..47
FT                   /note="Bacteriocin curvaticin DN317"
FT                   /evidence="ECO:0000269|PubMed:27812926"
FT                   /id="PRO_0000438928"
SQ   SEQUENCE   47 AA;  4448 MW;  329DB68EAB35623A CRC64;
     IPYGGNGVHH GKAGDSXTVD TAIGNIGNNA ASIIGGMISG WASGLAG