ID   CU36_MANSE              Reviewed;         342 AA.
AC   Q8MUC5;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Pupal cuticle protein 36;
DE   AltName: Full=MS-PCP36;
DE            Short=MsCP36;
DE   Flags: Precursor;
GN   Name=PCP36;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130 {ECO:0000312|EMBL:AAM88288.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 16-342, FUNCTION,
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MASS SPECTROMETRY.
RC   TISSUE=Pharate pupal cuticle {ECO:0000269|PubMed:12609518};
RX   PubMed=12609518; DOI=10.1016/s0965-1748(02)00247-3;
RA   Suderman R.J., Andersen S.O., Hopkins T.L., Kanost M.R., Kramer K.J.;
RT   "Characterization and cDNA cloning of three major proteins from pharate
RT   pupal cuticle of Manduca sexta.";
RL   Insect Biochem. Mol. Biol. 33:331-343(2003).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 16-47, AND SUBCELLULAR LOCATION.
RC   TISSUE=Pharate pupal cuticle {ECO:0000269|PubMed:10646967};
RX   PubMed=10646967; DOI=10.1016/s0965-1748(99)00091-0;
RA   Hopkins T.L., Krchma L.J., Ahmad S.A., Kramer K.J.;
RT   "Pupal cuticle proteins of Manduca sexta: characterization and profiles
RT   during sclerotization.";
RL   Insect Biochem. Mol. Biol. 30:19-27(2000).
CC   -!- FUNCTION: Component of the pupal abdominal endocuticle. May have
CC       important role in the larval and adult exoskeleton structure.
CC       {ECO:0000269|PubMed:12609518, ECO:0000303|PubMed:10646967}.
CC   -!- SUBCELLULAR LOCATION: Note=Synthesized in the epidermis during the
CC       larval-pupal transformation and then secreted into the pharate pupal
CC       cuticle. {ECO:0000269|PubMed:10646967, ECO:0000269|PubMed:12609518}.
CC   -!- DEVELOPMENTAL STAGE: Present at low levels early in the fifth larval
CC       stadium, followed by a large increase in abundance prior to pupal
CC       ecdysis. Also present at low levels in adults.
CC       {ECO:0000269|PubMed:12609518}.
CC   -!- MASS SPECTROMETRY: Mass=29771; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12609518};
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DR   EMBL; AF526296; AAM88288.1; -; mRNA.
DR   AlphaFoldDB; Q8MUC5; -.
DR   GO; GO:0042302; F:structural constituent of cuticle; IEA:UniProtKB-KW.
DR   InterPro; IPR031311; CHIT_BIND_RR_consensus.
DR   InterPro; IPR000618; Insect_cuticle.
DR   Pfam; PF00379; Chitin_bind_4; 1.
DR   PRINTS; PR00947; CUTICLE.
DR   PROSITE; PS00233; CHIT_BIND_RR_1; 1.
DR   PROSITE; PS51155; CHIT_BIND_RR_2; 1.
PE   1: Evidence at protein level;
KW   Cuticle; Direct protein sequencing; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000269|PubMed:10646967,
FT                   ECO:0000269|PubMed:12609518"
FT   CHAIN           16..342
FT                   /note="Pupal cuticle protein 36"
FT                   /evidence="ECO:0000269|PubMed:12609518"
FT                   /id="PRO_0000006412"
FT   DOMAIN          135..198
FT                   /note="Chitin-binding type R&R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT   REGION          322..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        328..342
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        201
FT                   /note="P -> I (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        234
FT                   /note="V -> G (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        270
FT                   /note="T -> A (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   342 AA;  31168 MW;  181C679197C07AB0 CRC64;
     MKLFVLAAVL GVCLADRLDN KYLPPRGNAG AGFGPGFGGA GPKGGGSGFG GAGSGFGGAG
     SGFGGAGSGF GGGAGGAFGH GGAGSGFGGQ AGGYSGAGGA GGYSGGASGQ YSGRGGAASA
     DANAQILRLN NEVTAEGFAY DFETSNGIRA DAQGVATNGV QSQGSFAYKG DDGQDYSITY
     TADENGFVPQ GAHLPTPPPI PEEILKSLEQ NARDEAAGIV DDGTYRGEGA GAGVAGGYSG
     AGGYSGGAGG AGGFGAGGAG RAGGAGGFGT GGAGRAGGAG GFGAGGAGGA GGAGGFGAGA
     GGFGGRGSGG FGGAASRQYL APNAGGRGSG SGNFNAQTGY QY