ID   CU5A_CONCL              Reviewed;          76 AA.
AC   D2Y171;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Conotoxin Cal5a L1 {ECO:0000303|PubMed:21172372};
DE   Contains:
DE     RecName: Full=Conotoxin Cal5b L1 {ECO:0000303|PubMed:21172372};
DE   Contains:
DE     RecName: Full=Conotoxin Cal5.1 {ECO:0000303|PubMed:21172372};
DE   Flags: Precursor;
OS   Californiconus californicus (California cone) (Conus californicus).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Californiconus.
OX   NCBI_TaxID=1736779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], HYDROXYLATION AT PRO-50; PRO-58; PRO-62 AND
RP   PRO-64, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=21172372; DOI=10.1016/j.toxicon.2010.12.008;
RA   Elliger C.A., Richmond T.A., Lebaric Z.N., Pierce N.T., Sweedler J.V.,
RA   Gilly W.F.;
RT   "Diversity of conotoxin types from Conus californicus reflects a diversity
RT   of prey types and a novel evolutionary history.";
RL   Toxicon 57:311-322(2011).
CC   -!- FUNCTION: Probable neurotoxin with unknown target. Possibly targets ion
CC       channels. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21172372}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:21172372}.
CC   -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC   -!- PTM: Contains 2 disulfide bonds that can be either 'C1-C3, C2-C4' or
CC       'C1-C4, C2-C3', since these disulfide connectivities have been observed
CC       for conotoxins with cysteine framework V (for examples, see AC P0DQQ7
CC       and AC P81755). {ECO:0000305}.
CC   -!- PTM: Five different peptides have been described after total venom
CC       examination by HPLC-MS. Cal5a is the longest. Cal5b-Cal5e are identical
CC       in length but are differentially hydroxylated. It is possible that
CC       hydroxylation and proteolysis at position 53 are incomplete in some of
CC       these peptides. {ECO:0000269|PubMed:21172372}.
CC   -!- MISCELLANEOUS: Cal5a-cal5e are found in dissected venom (DV).
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DR   EMBL; GU290201; ADB43128.1; -; mRNA.
DR   AlphaFoldDB; D2Y171; -.
DR   ConoServer; 3983; Cal5.1 L1 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Disulfide bond; Hydroxylation; Ion channel impairing toxin; Neurotoxin;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..42
FT                   /evidence="ECO:0000305|PubMed:21172372"
FT                   /id="PRO_5000566287"
FT   PEPTIDE         44..74
FT                   /note="Conotoxin Cal5a L1"
FT                   /evidence="ECO:0000305|PubMed:21172372"
FT                   /id="PRO_0000414960"
FT   PEPTIDE         54..74
FT                   /note="Conotoxin Cal5b L1"
FT                   /evidence="ECO:0000305|PubMed:21172372"
FT                   /id="PRO_0000414961"
FT   PEPTIDE         61..74
FT                   /note="Conotoxin Cal5.1"
FT                   /evidence="ECO:0000305|PubMed:21172372"
FT                   /id="PRO_5000566288"
FT   MOD_RES         50
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:21172372"
FT   MOD_RES         58
FT                   /note="4-hydroxyproline; in form cal5a, and form cal5b"
FT                   /evidence="ECO:0000269|PubMed:21172372"
FT   MOD_RES         62
FT                   /note="4-hydroxyproline; in form cal5a, form cal5b, and
FT                   form cal5c"
FT                   /evidence="ECO:0000269|PubMed:21172372"
FT   MOD_RES         64
FT                   /note="4-hydroxyproline; in form cal5a, form cal5b, form
FT                   cal5c, and form cal5d"
FT                   /evidence="ECO:0000269|PubMed:21172372"
SQ   SEQUENCE   76 AA;  8272 MW;  87FDBC5169A78EC6 CRC64;
     MRFYIGLMAA LMLTSILRTD SASVDQTGAE GGLALIERVI RQRDAADVKP VARTNEGPGR
     DPAPCCQHPI ETCCRR