CUB1_YEAST
ID CUB1_YEAST Reviewed; 551 AA.
AC Q08977; D6W3A9; Q05760; Q7LGU3;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Cu(2+) suppressing and bleomycin sensitive protein 1;
GN Name=CUB1; OrderedLocusNames=YPL260W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-156.
RX PubMed=3040736; DOI=10.1016/s0021-9258(18)45347-1;
RA Wu M., Tzagoloff A.;
RT "Mitochondrial and cytoplasmic fumarases in Saccharomyces cerevisiae are
RT encoded by a single nuclear gene FUM1.";
RL J. Biol. Chem. 262:12275-12282(1987).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP PHOSPHORYLATION.
RX PubMed=16172400; DOI=10.1073/pnas.0501046102;
RA Budovskaya Y.V., Stephan J.S., Deminoff S.J., Herman P.K.;
RT "An evolutionary proteomics approach identifies substrates of the cAMP-
RT dependent protein kinase.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13933-13938(2005).
RN [6]
RP INDUCTION.
RX PubMed=22842922; DOI=10.1038/ncb2549;
RA Tkach J.M., Yimit A., Lee A.Y., Riffle M., Costanzo M., Jaschob D.,
RA Hendry J.A., Ou J., Moffat J., Boone C., Davis T.N., Nislow C., Brown G.W.;
RT "Dissecting DNA damage response pathways by analysing protein localization
RT and abundance changes during DNA replication stress.";
RL Nat. Cell Biol. 14:966-976(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBUNIT.
RX DOI=10.1016/j.plgene.2015.11.002;
RA Firestone K., Awonusi D., Panfair D., Roland D., Ramamurthy A.,
RA Kusmierczyk A.R.;
RT "YPL260W, a high-copy suppressor of a copper-sensitive phenotype in yeast,
RT is linked to DNA repair and proteasome function.";
RL Plant Gene 5:38-48(2016).
CC -!- FUNCTION: Involved in bleomycin tolerance with links to DNA repair
CC and/or proteasome function. {ECO:0000269|Ref.7}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|Ref.7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- INDUCTION: Expression is induced in response to DNA replication stress.
CC {ECO:0000269|PubMed:22842922}.
CC -!- PTM: Phosphorylated by PKA in vitro. {ECO:0000269|PubMed:16172400}.
CC -!- DISRUPTION PHENOTYPE: Leads to sensitivity to bleomycin (Ref.7).
CC Bleomycin sensnsitivity is even increased when CUB1 deletion is
CC combined with proteasome mutants including PRE9 or UMP1 disruptions
CC (Ref.7). Suppresses the copper tolerance induced by the proteasome
CC subunit PRE9 disuption (Ref.7). Leads to synthetic sickness with
CC deletion of RAD6 on media containing bleomycin, but not hydroxyurea
CC (HU) (Ref.7). {ECO:0000269|Ref.7}.
CC -!- SIMILARITY: Belongs to the CUB1 family. {ECO:0000305}.
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DR EMBL; Z73615; CAA97990.1; -; Genomic_DNA.
DR EMBL; Z73617; CAA97996.1; -; Genomic_DNA.
DR EMBL; J02802; AAA66910.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11175.1; -; Genomic_DNA.
DR PIR; S65293; S65293.
DR RefSeq; NP_015063.1; NM_001184074.1.
DR AlphaFoldDB; Q08977; -.
DR SMR; Q08977; -.
DR BioGRID; 35952; 113.
DR DIP; DIP-2002N; -.
DR IntAct; Q08977; 4.
DR MINT; Q08977; -.
DR STRING; 4932.YPL260W; -.
DR iPTMnet; Q08977; -.
DR MaxQB; Q08977; -.
DR PaxDb; Q08977; -.
DR PRIDE; Q08977; -.
DR EnsemblFungi; YPL260W_mRNA; YPL260W; YPL260W.
DR GeneID; 855867; -.
DR KEGG; sce:YPL260W; -.
DR SGD; S000006181; CUB1.
DR VEuPathDB; FungiDB:YPL260W; -.
DR eggNOG; ENOG502QPV0; Eukaryota.
DR HOGENOM; CLU_026648_1_0_1; -.
DR InParanoid; Q08977; -.
DR OMA; KQDRTTY; -.
DR BioCyc; YEAST:G3O-34145-MON; -.
DR PRO; PR:Q08977; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q08977; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR InterPro; IPR018810; UPF0662.
DR PANTHER; PTHR28086; PTHR28086; 1.
DR Pfam; PF10303; DUF2408; 3.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..551
FT /note="Cu(2+) suppressing and bleomycin sensitive protein
FT 1"
FT /id="PRO_0000255978"
FT REGION 513..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 174..213
FT /evidence="ECO:0000255"
FT COILED 249..300
FT /evidence="ECO:0000255"
FT COMPBIAS 535..551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 90
FT /note="D -> G (in Ref. 3; AAA66910)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="E -> K (in Ref. 3; AAA66910)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 551 AA; 62780 MW; 9DF384D00D58EDC8 CRC64;
MFASAGQQHP QIVPKEEESI LNYLLEVRSS LAKLKQNRTQ YLNSKDVQTT YQHVLTKVRE
LDDIRKNSHE TPAKSAATLI HSTELHNRVD SVLDDVFQLL SLCFLTVGLK NSAPATYASL
STVESLLEHL NESNVFTHHD LSPIKERLEE ISKIVEQKNS SPAYDEDGND DRLREIDNER
KKNKIEEDLL LRAKLKHCKD EYDILEGKLE EIDPSLSTVM EKLFRIRRGL LSLVASAKKT
MSKSDINTNS LLQEQNDLQT NNESLTDDKH LVSQEYVHEK LSVLKNELSE LESNRDDSGK
FKSLESHQVA EKGQSVLNGL LDDCHDLVND LSHQKNGGLT LDPYLQPIYE QLIDIKTTLE
NLMITRRWTL RETDLFSYQK KLNEIDNKRI NGKFPTKSQD SKGQSILLYL LRRCYAIIYK
LLESSEPVSE ALQPIHNQLS TVRRCLLELK RMGGVNNERE LYPYQMKLAS LDNLRTEGIF
YDSDGNIPEG QGILNALLAE CFDILHELKV EAEEKAQNST SSDGSDDDDN GESGIDSNSN
DSEPESEYQQ E
