CUDA_DICDI
ID CUDA_DICDI Reviewed; 802 AA.
AC O00841; Q54PJ0;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Putative transcriptional regulator cudA;
GN Name=cudA; ORFNames=DDB_G0284465;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=9226443; DOI=10.1242/dev.124.14.2719;
RA Fukuzawa M., Hopper N., Williams J.;
RT "CudA: a Dictyostelium gene with pleiotropic effects on cellular
RT differentiation and slug behaviour.";
RL Development 124:2719-2728(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION BY DSTA.
RX PubMed=10821768; DOI=10.1242/dev.127.12.2705;
RA Fukuzawa M., Williams J.G.;
RT "Analysis of the promoter of the cudA gene reveals novel mechanisms of
RT Dictyostelium cell type differentiation.";
RL Development 127:2705-2713(2000).
RN [4]
RP INDUCTION BY ACAA.
RX PubMed=11356026; DOI=10.1006/dbio.2001.0232;
RA Verkerke-van Wijk I., Fukuzawa M., Devreotes P.N., Schaap P.;
RT "Adenylyl cyclase A expression is tip-specific in Dictyostelium slugs and
RT directs StatA nuclear translocation and CudA gene expression.";
RL Dev. Biol. 234:151-160(2001).
CC -!- FUNCTION: Essential for normal culmination. May function as a
CC transcriptional regulator. {ECO:0000269|PubMed:9226443}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:9226443}.
CC -!- TISSUE SPECIFICITY: Expressed in the prestalk cells that constitute the
CC slug tip (pstA cells) and in prespore cells (at protein level). Not
CC expressed in the band of prestalk cells that lies behind the slug tip
CC (pstO cells). Highly expressed in pstO derived papilla cells during
CC culmination. {ECO:0000269|PubMed:9226443}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during aggregation and remains
CC constant throughout culmination. {ECO:0000269|PubMed:9226443}.
CC -!- INDUCTION: Induced by acaA in the slug tip. Induced by dstA in prestalk
CC cells, but not in prespore cells. {ECO:0000269|PubMed:10821768,
CC ECO:0000269|PubMed:11356026}.
CC -!- DISRUPTION PHENOTYPE: Mutants fail to culminate. Development is normal
CC until migratory slugs are formed, but slugs lacking cudA fail to
CC culminate in the dark or in low level unidirectional light. In overhead
CC light slugs lacking cudA eventually form defective fruiting bodies that
CC lack both mature stalk cells and mature spore cells.
CC {ECO:0000269|PubMed:9226443}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y13119; CAA73586.1; -; mRNA.
DR EMBL; AAFI02000066; EAL65159.1; -; Genomic_DNA.
DR RefSeq; XP_638542.1; XM_633450.1.
DR AlphaFoldDB; O00841; -.
DR STRING; 44689.DDB0191385; -.
DR PaxDb; O00841; -.
DR EnsemblProtists; EAL65159; EAL65159; DDB_G0284465.
DR GeneID; 8624635; -.
DR KEGG; ddi:DDB_G0284465; -.
DR dictyBase; DDB_G0284465; cudA.
DR eggNOG; ENOG502RBCU; Eukaryota.
DR HOGENOM; CLU_351064_0_0_1; -.
DR InParanoid; O00841; -.
DR OMA; AFKINTC; -.
DR PRO; PR:O00841; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:dictyBase.
DR GO; GO:0030154; P:cell differentiation; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:dictyBase.
DR GO; GO:0097696; P:receptor signaling pathway via STAT; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:UniProtKB.
DR InterPro; IPR040430; CudA-like.
DR PANTHER; PTHR38092; PTHR38092; 2.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Sporulation; Transcription; Transcription regulation.
FT CHAIN 1..802
FT /note="Putative transcriptional regulator cudA"
FT /id="PRO_0000079557"
FT REGION 1..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 636..658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 90
FT /note="T -> A (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="N -> S (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="Missing (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
FT CONFLICT 447
FT /note="N -> T (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
FT CONFLICT 671..676
FT /note="Missing (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
FT CONFLICT 754..757
FT /note="Missing (in Ref. 1; CAA73586)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 802 AA; 91743 MW; 62140CA14005533F CRC64;
MNQSQNFHNI DLGHYGAQGS NQSFNNNNNG NNGMMMNQQQ MQQHVVPHLH HLQQQQQQPQ
QQQLRNVPDY SNSPNGTTNG STMSPNCINT NNNNNNNNNN NNNSNNNNNN NNNASNNLTS
NKSSSTNTPQ IGQLQASPAN LTNSPSAISS PITISNNSSL NSPSTTSSPN LLLNGTSNKR
IMISQQTCLV EEKFSKNGVQ KNVHVVVKNN PFLLTLSLLD SSLNFHQLTP EVQLVYDSES
LKEVDSATVK PLEYKTRANE EGDQLTIELR IKVLSSQLED MLFRAKVKIV DPRTRKETHG
LSVITHPIRV VSKPDQVKKK AKKRKRAPTD SLMDTLNRIE HQQKEQQRLL KKLCYHDKEN
NIIQLIQQQQ QQQQLLNNVT NNINNNNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
NTTSTTTTTT TTTSSCNNNN NNNNENNEHI VKIENTECNN NNNNIINNTE NDENINKPIL
NSKDEFQSAF KEFIGAFKQL QCLDPDGADG AFKINTCAND AQTMCEILEM VKVELKKDEN
FKDKCGGSSG GACGENNPDN PCSCKVCPYK QKVDHINQSY ETYFNMFNPS NSNSVVPQQS
QQLQQQQIQQ QQQSQQQVQQ QQQQQMQQQP QQQQQQPQQQ QQNQQQGQQP QQQQQQGQLD
YTTYIDPQLQ MQQQLQMQQA AQQQYMQQTM DQQQQQQYYM QQYHLQQQQQ QQQAQRYLMQ
QQYMQQQAQQ QQQQHQQVAI QQQQQQNQQQ NQQQNQQQQN QSPQNQQSLD FQNANNFIDF
NSGLGFMNFP NINFGDMGFS AV
