CUER_SALTY
ID CUER_SALTY Reviewed; 138 AA.
AC Q93CH6;
DT 03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=HTH-type transcriptional regulator CueR;
DE AltName: Full=Copper efflux regulator;
DE AltName: Full=Copper export regulator;
GN Name=cueR; Synonyms=scr; OrderedLocusNames=STM0499;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Choi S.-Y., Lee I., Kim J.;
RT "SCR of Salmonella typhimurium is a copper-responsive MerR family
RT transcriptional regulator of CuiD.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP FUNCTION IN COPPER TOLERANCE, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=17768242; DOI=10.1099/mic.0.2007/006536-0;
RA Espariz M., Checa S.K., Perez Audero M.E., Pontel L.B., Soncini F.C.;
RT "Dissecting the Salmonella response to copper.";
RL Microbiology 153:2989-2997(2007).
CC -!- FUNCTION: Regulates the transcription of the copA and cuiD (cueO)
CC genes. Detects cytoplasmic copper stress and activates transcription in
CC response to increasing copper concentrations (PubMed:17768242).
CC {ECO:0000269|PubMed:17768242}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: It contains a N-terminal DNA binding region and a C-terminal
CC metal binding region. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Loss of copper-based induction of copA. Causes a
CC mild defect in aerobic growth on CuSO(4), strongly impairs survival
CC during anaerobic growth on CuSO(4). {ECO:0000269|PubMed:17768242}.
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DR EMBL; AF409088; AAL11724.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL19453.1; -; Genomic_DNA.
DR RefSeq; NP_459494.1; NC_003197.2.
DR RefSeq; WP_001026760.1; NC_003197.2.
DR AlphaFoldDB; Q93CH6; -.
DR SMR; Q93CH6; -.
DR STRING; 99287.STM0499; -.
DR PaxDb; Q93CH6; -.
DR EnsemblBacteria; AAL19453; AAL19453; STM0499.
DR GeneID; 1252019; -.
DR KEGG; stm:STM0499; -.
DR PATRIC; fig|99287.12.peg.533; -.
DR HOGENOM; CLU_060077_2_0_6; -.
DR OMA; FNDPERH; -.
DR PhylomeDB; Q93CH6; -.
DR BioCyc; SENT99287:STM0499-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd01108; HTH_CueR; 1.
DR InterPro; IPR011789; CueR.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR000551; MerR-type_HTH_dom.
DR Pfam; PF13411; MerR_1; 1.
DR PRINTS; PR00040; HTHMERR.
DR SMART; SM00422; HTH_MERR; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
DR TIGRFAMs; TIGR02044; CueR; 1.
DR PROSITE; PS00552; HTH_MERR_1; 1.
DR PROSITE; PS50937; HTH_MERR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Copper; Cytoplasm; DNA-binding; Metal-binding;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..138
FT /note="HTH-type transcriptional regulator CueR"
FT /id="PRO_0000098115"
FT DOMAIN 1..69
FT /note="HTH merR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT DNA_BIND 4..23
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT BINDING 112
FT /ligand="Cu(+)"
FT /ligand_id="ChEBI:CHEBI:49552"
FT /evidence="ECO:0000250"
FT BINDING 120
FT /ligand="Cu(+)"
FT /ligand_id="ChEBI:CHEBI:49552"
FT /evidence="ECO:0000250"
SQ SEQUENCE 138 AA; 15648 MW; 20D6D1C23EA6D850 CRC64;
MNISDVAKKT GLTSKAIRFY EEKGLVTPPL RSENGYRTYT QKHLNELTLL RQARQVGFNL
EECGELVNLF NDPRRHSADV KKRTLEKVAE IERHISELQS MRDQLLALAE SCPGDDSADC
PIIDNLSGCC HHKAQKPR
