ID   CUER_VIBC3              Reviewed;         139 AA.
AC   A5F2W6; C3LYY5; Q9EVT1; Q9KTC8;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=HTH-type transcriptional regulator CueR;
DE   AltName: Full=Copper efflux regulator;
DE   AltName: Full=Copper export regulator;
GN   Name=cueR; Synonyms=vvgR; OrderedLocusNames=VC0395_A0495, VC395_0989;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Srivastava B.S.;
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Regulates the transcription of the copA and cueO genes. It
CC       detects cytoplasmic copper stress and activates transcription in
CC       response to increasing copper concentrations (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: It contains a N-terminal DNA binding region and a C-terminal
CC       metal binding region. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC21398.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ277893; CAC21398.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP000627; ABQ20141.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP09001.1; -; Genomic_DNA.
DR   RefSeq; WP_001027396.1; NZ_JAACZH010000005.1.
DR   AlphaFoldDB; A5F2W6; -.
DR   SMR; A5F2W6; -.
DR   STRING; 345073.VC395_0989; -.
DR   EnsemblBacteria; ABQ20141; ABQ20141; VC0395_A0495.
DR   GeneID; 57739662; -.
DR   KEGG; vco:VC0395_A0495; -.
DR   KEGG; vcr:VC395_0989; -.
DR   PATRIC; fig|345073.21.peg.958; -.
DR   eggNOG; COG0789; Bacteria.
DR   HOGENOM; CLU_060077_2_0_6; -.
DR   OMA; HQSCASA; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd01108; HTH_CueR; 1.
DR   InterPro; IPR011789; CueR.
DR   InterPro; IPR009061; DNA-bd_dom_put_sf.
DR   InterPro; IPR000551; MerR-type_HTH_dom.
DR   Pfam; PF13411; MerR_1; 1.
DR   SMART; SM00422; HTH_MERR; 1.
DR   SUPFAM; SSF46955; SSF46955; 1.
DR   TIGRFAMs; TIGR02044; CueR; 1.
DR   PROSITE; PS00552; HTH_MERR_1; 1.
DR   PROSITE; PS50937; HTH_MERR_2; 1.
PE   3: Inferred from homology;
KW   Activator; Copper; Cytoplasm; DNA-binding; Metal-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..139
FT                   /note="HTH-type transcriptional regulator CueR"
FT                   /id="PRO_0000321855"
FT   DOMAIN          1..69
FT                   /note="HTH merR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   DNA_BIND        4..23
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   BINDING         112
FT                   /ligand="Cu(+)"
FT                   /ligand_id="ChEBI:CHEBI:49552"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="Cu(+)"
FT                   /ligand_id="ChEBI:CHEBI:49552"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   139 AA;  15693 MW;  84CEDED9D411B012 CRC64;
     MNISQIAKLT SLTAKSIRLY EEKGLIIPPL RSESGYRTYT QQHVDDLLLI ARCRRVGFSL
     DECKAMLTLA NDPNRTSAAV RARAQEKWQE ISRKLSELTM IKQQLEEWIA SCPGDQGSDC
     PIIEQLKGHC CSNNKTKTP