ID   CUGA_CONPB              Reviewed;          11 AA.
AC   P86942; I3NI55;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   02-JUN-2021, entry version 20.
DE   RecName: Full=Conotoxin pc16a {ECO:0000303|PubMed:22079220};
DE   AltName: Full=pc16b;
OS   Conus pictus (Cone snail).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Sciteconus.
OX   NCBI_TaxID=1042615;
RN   [1]
RP   PROTEIN SEQUENCE, POTENTIAL FUNCTION, DISULFIDE BOND, MASS SPECTROMETRY,
RP   DOMAIN, AMIDATION AT CYS-11, STRUCTURE BY NMR, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=22079220; DOI=10.1016/j.peptides.2011.10.026;
RA   Van Der Haegen A., Peigneur S., Dyubankova N., Moller C., Mari F.,
RA   Diego-Garcia E., Naude R., Lescrinier E., Herdewijn P., Tytgat J.;
RT   "Pc16a, the first characterized peptide from Conus pictus venom, shows a
RT   novel disulfide connectivity.";
RL   Peptides 34:106-113(2012).
RN   [2]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=22776330; DOI=10.1016/j.peptides.2012.07.002;
RA   Peigneur S., Van Der Haegen A., Moller C., Waelkens E., Diego-Garcia E.,
RA   Mari F., Naude R., Tytgat J.;
RT   "Unraveling the peptidome of the South African cone snails Conus pictus and
RT   Conus natalis.";
RL   Peptides 41:8-16(2013).
CC   -!- FUNCTION: Potential neurotoxin. No target has been identified for this
CC       peptide. {ECO:0000269|PubMed:22079220}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22079220,
CC       ECO:0000269|PubMed:22776330}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:22079220, ECO:0000305|PubMed:22776330}.
CC   -!- DOMAIN: The cysteine framework is XVI (C-C-CC). {ECO:0000305}.
CC   -!- PTM: Pc16a is the amidated form, whereas Pc16b is the non-amidated
CC       form. {ECO:0000269|PubMed:22776330}.
CC   -!- MASS SPECTROMETRY: Mass=1257.6; Mass_error=0.7; Method=Electrospray;
CC       Note=monoisotopic, amidated form.;
CC       Evidence={ECO:0000269|PubMed:22079220, ECO:0000269|PubMed:22776330};
CC   -!- MASS SPECTROMETRY: Mass=1258.6; Method=Electrospray; Note=monoisotopic,
CC       non-amidated form.; Evidence={ECO:0000269|PubMed:22776330};
CC   -!- MISCELLANEOUS: The native peptide has no effect on nicotinic
CC       acetylcholine receptor (nAChR) subtypes human alpha-4-beta-2/CHRNA4-
CC       CHRNB2 and chicken alpha-7/CHRNA7. The synthetic peptide has no effect
CC       on a wide range of mammalian and insect voltage-gated sodium channels
CC       and voltage-gated potassium channels (PubMed:22079220).
CC       {ECO:0000269|PubMed:22079220}.
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DR   PDB; 2LER; NMR; -; A=1-11.
DR   PDBsum; 2LER; -.
DR   SMR; P86942; -.
DR   ConoServer; 5219; Pc16a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Amidation; Direct protein sequencing; Disulfide bond;
KW   Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..11
FT                   /note="Conotoxin pc16a"
FT                   /evidence="ECO:0000269|PubMed:22079220"
FT                   /id="PRO_0000419007"
FT   MOD_RES         11
FT                   /note="Cysteine amide; partial"
FT                   /evidence="ECO:0000269|PubMed:22079220"
FT   DISULFID        2..10
FT                   /evidence="ECO:0000269|PubMed:22079220,
FT                   ECO:0007744|PDB:2LER"
FT   DISULFID        4..11
FT                   /evidence="ECO:0000269|PubMed:22079220,
FT                   ECO:0007744|PDB:2LER"
FT   TURN            4..6
FT                   /evidence="ECO:0007829|PDB:2LER"
FT   HELIX           8..10
FT                   /evidence="ECO:0007829|PDB:2LER"
SQ   SEQUENCE   11 AA;  1264 MW;  87337EF6C454033E CRC64;
     SCSCKRNFLC C