ID   CULP5_MYCTU             Reviewed;         174 AA.
AC   O06793; I6YBJ4; Q7D805;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Probable carboxylesterase Culp5 {ECO:0000305};
DE            EC=3.1.1.- {ECO:0000250|UniProtKB:P9WP43};
DE   AltName: Full=Cutinase-like protein 5 {ECO:0000303|PubMed:19225166};
DE            Short=Culp5 {ECO:0000303|PubMed:19225166};
GN   Name=cut1 {ECO:0000312|EMBL:CCP44524.1};
GN   OrderedLocusNames=Rv1758 {ECO:0000312|EMBL:CCP44524.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   SHOWS THAT IT DOES NOT HAVE CUTINASE ACTIVITY.
RC   STRAIN=H37Rv;
RX   PubMed=19225166; DOI=10.1096/fj.08-114421;
RA   West N.P., Chow F.M., Randall E.J., Wu J., Chen J., Ribeiro J.M.,
RA   Britton W.J.;
RT   "Cutinase-like proteins of Mycobacterium tuberculosis: characterization of
RT   their variable enzymatic functions and active site identification.";
RL   FASEB J. 23:1694-1704(2009).
CC   -!- FUNCTION: Does not exhibit cutinase activity.
CC       {ECO:0000269|PubMed:19225166}.
CC   -!- SIMILARITY: Belongs to the cutinase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44524.1; -; Genomic_DNA.
DR   RefSeq; NP_216274.1; NC_000962.3.
DR   RefSeq; WP_009934708.1; NC_000962.3.
DR   AlphaFoldDB; O06793; -.
DR   SMR; O06793; -.
DR   STRING; 83332.Rv1758; -.
DR   ESTHER; myctu-RV1758; Cutinase.
DR   PaxDb; O06793; -.
DR   DNASU; 885552; -.
DR   GeneID; 885552; -.
DR   KEGG; mtu:Rv1758; -.
DR   PATRIC; fig|83332.111.peg.1956; -.
DR   TubercuList; Rv1758; -.
DR   eggNOG; ENOG5030PZC; Bacteria.
DR   OMA; PPINIGH; -.
DR   PhylomeDB; O06793; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000675; Cutinase/axe.
DR   PANTHER; PTHR33630; PTHR33630; 1.
DR   Pfam; PF01083; Cutinase; 1.
DR   SMART; SM01110; Cutinase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..174
FT                   /note="Probable carboxylesterase Culp5"
FT                   /id="PRO_0000450108"
FT   ACT_SITE        67
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O53581"
FT   ACT_SITE        141
FT                   /evidence="ECO:0000250|UniProtKB:O53581"
FT   ACT_SITE        153
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:O53581"
FT   SITE            68
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:P00590"
FT   DISULFID        137..144
FT                   /evidence="ECO:0000250|UniProtKB:O53581"
SQ   SEQUENCE   174 AA;  17868 MW;  36684DEB29AE73C8 CRC64;
     MPGRFREDFI DALRSKIGEK SMGVYGVDYP ATTDFPTAMA GIYDAGTHVE QTAANCPQSK
     LVLGGFSQGA AVMGFVTAAA IPDGAPLDAP RPMPPEVADH VAAVTLFGMP SVAFMHSIGA
     PPIVIGPLYA EKTIQLCAPG DPVCSSGGNW AAHNGYADDG MVEQAAVFAA GRLG