CUSA_ECO57
ID CUSA_ECO57 Reviewed; 1045 AA.
AC Q8XBY1;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Cation efflux system protein CusA;
GN Name=cusA; OrderedLocusNames=Z0714, ECs0613;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Part of a cation efflux system that mediates resistance to
CC copper and silver. {ECO:0000250}.
CC -!- SUBUNIT: The cus efflux system is composed of CusA, CusB, CusC and
CC CusF. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: Transcriptionally regulated by CusR in response to copper
CC and silver ions. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
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DR EMBL; AE005174; AAG54908.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB34036.1; -; Genomic_DNA.
DR PIR; E90705; E90705.
DR PIR; H85555; H85555.
DR RefSeq; NP_308640.1; NC_002695.1.
DR RefSeq; WP_000573920.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8XBY1; -.
DR SMR; Q8XBY1; -.
DR STRING; 155864.EDL933_0639; -.
DR EnsemblBacteria; AAG54908; AAG54908; Z0714.
DR EnsemblBacteria; BAB34036; BAB34036; ECs_0613.
DR GeneID; 916972; -.
DR KEGG; ece:Z0714; -.
DR KEGG; ecs:ECs_0613; -.
DR PATRIC; fig|386585.9.peg.721; -.
DR eggNOG; COG3696; Bacteria.
DR HOGENOM; CLU_002755_1_2_6; -.
DR OMA; NSWTYPI; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006825; P:copper ion transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004763; CzcA/CusA/SilA/NccA/HelA/CnrA.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00914; 2A0601; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Copper; Copper transport;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..1045
FT /note="Cation efflux system protein CusA"
FT /id="PRO_0000161816"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 869..889
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 896..916
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 926..946
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 983..1003
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1010..1030
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1045 AA; 114538 MW; 3DC0AAE0F8C5278D CRC64;
MIEWIIRRSV ANRFLVLMGA LFLSIWGTWT IINTPVDALP DLSDVQVIIK TSYPGQAPQI
VENQVTYPLT TTMLSVPGAK TVRGFSQFGD SYVYVIFEDG TDPYWARSRV LEYLNQVQGK
LPAGVSAELG PDATGVGWIY EYALVDRSGK HDLADLRSLQ DWFLKYELKT IPDVAEVASV
GGVVKEYQVV IDPQRLAQYG ISLAEVKSAL DASNQEAGGS SIELAEAEYM VRASGYLQTL
DDFNHIVLKA SENGVPVYLR DVAKIQVGPE MRRGIAELNG EVVGGVVILR SGKNAREVIA
AVKDKLETLK SSLPEGVEIV TTYDRSQLID RAIDNLSGKL LEEFIVVAVV CALFLWHVRS
ALVAIISLPL GLCIAFIVMH FQGLNANIMS LGGIAIAVGA MVDAAIVMIE NAHKRLEEWQ
HQHPDATLDN KTRWQVITNA SVEVGPALFI SLLIITLSFI PIFTLEGQEG RLFGPLAFTK
TYAMAGAALL AIVVIPILMG YWIRGKIPPE SSNPLNRFLI RVYHPLLLKV LHWPKTTLLV
AALSVLTVLW PLNKVGGEFL PQINEGDLLY MPSTLPGISA AEAASMLQKT DKLIMSVPEV
ARVFGKTGKA ETATDSAPLE MVETTIQLKP QEQWRPGMTM DKIIEELDNT VRLPGLANLW
VPPIRNRIDM LSTGIKSPIG IKVSGTVLAD IDAMAEQIEE VARTVPGVAS ALAERLEGGR
YINVEINREK AARYGMTVAD VQLFVTSAVG GAMVGETVEG IARYPINLRY PQSWRDSPQA
LRQLPILTPM KQQITLADVA DVKVSTGPSM LKTENARPTS WIYIDARDRD MVSVVHDLQK
AIAEKVQLKP GTSVAFSGQF ELLERANHKL KLMVPMTLMI IFVLLYLAFR RVGEALLIIS
SVPFALVGGI WLLWWMGFHL SVATGTGFIA LAGVAAEFGV VMLMYLRHAI EAEPSLNNPQ
TFSEQKLDEA LHHGAVLRVR PKAMTVAVII AGLLPILWGT GAGSEVMSRI AAPMIGGMIT
APLLSLFIIP AAYKLMWLHR HRVRK
