ID   CUSA_ECOL6              Reviewed;        1047 AA.
AC   Q8FK36;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Cation efflux system protein CusA;
GN   Name=cusA; OrderedLocusNames=c0661;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Part of a cation efflux system that mediates resistance to
CC       copper and silver. {ECO:0000250}.
CC   -!- SUBUNIT: The cus efflux system is composed of CusA, CusB, CusC and
CC       CusF. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Transcriptionally regulated by CusR in response to copper
CC       and silver ions. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN79136.1; -; Genomic_DNA.
DR   RefSeq; WP_000573985.1; NC_004431.1.
DR   STRING; 199310.c0661; -.
DR   EnsemblBacteria; AAN79136; AAN79136; c0661.
DR   KEGG; ecc:c0661; -.
DR   eggNOG; COG3696; Bacteria.
DR   HOGENOM; CLU_002755_1_2_6; -.
DR   OMA; NSWTYPI; -.
DR   BioCyc; ECOL199310:C0661-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006825; P:copper ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004763; CzcA/CusA/SilA/NccA/HelA/CnrA.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00914; 2A0601; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Copper; Copper transport;
KW   Ion transport; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1047
FT                   /note="Cation efflux system protein CusA"
FT                   /id="PRO_0000161817"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..358
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        363..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        390..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        446..466
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        485..505
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        532..552
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        898..918
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        928..948
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        985..1005
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1012..1032
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1047 AA;  114723 MW;  53F21ECED055930C CRC64;
     MIEWIIRRSV ANRFLVLMGA LFLSIWGTWT IINTPVDALP DLSDVQVIIK TSYPGQAPQI
     VENQVTYPLT TTMLSVPGAK TVRGFSQFGD SYVYVIFEDG TDPYWARSRV LEYLNQVQGK
     LPAGVSAELG PDATGVGWIY EYALVDRSGK HDLADLRSLQ DWFLKYELKT IPDVAEVASV
     GGVVKEYQVV IDPQRLAQYG ISLAEVKSAL DASNQEAGGS SIELAEAEYM VRASGYLQTL
     DDFNHIVLKA SENGVPVYLR DVAKVQVGPE MRRGIAELNG EGEVAGGVVI LRSGKNAREV
     IAAVKDKLET LKSSLPEGVE IVTTYDRSQL IDRAIDNLSG KLLEEFIVVA VVCALFLWHV
     RSALVAIISL PLGLCIAFIV MHFQGLNANI MSLGGIAIAV GAMVDAAIVM IENAHKRLEE
     WQHQHPDATL DNKTRWQVIT DASVEVGPAL FISLLIITLS FIPIFTLEGQ EGRLFGPLAF
     TKTYAMAGAA LLAIVVIPIL MGYWIRGKIP PESSNPLNRF LIRVYHPLLL KVLHWPKTTL
     LVAALSVLTV LWPXNKVGGE FLPQINEGDL LYMPSTLPGI SAAEAASMLQ KTDKLIMSVP
     EVARVFGKTG KAETATDSAP LEMVETTIQL KPQDQWRPGM TMDKIIEELD NTVRLPGLAN
     LWVPPIRNRI DMLSTGIKSP IGIKVSGTVL ADIDTMAEQI EEVARTVPGV ASALAERLEG
     GRYINVEINR EKAARYGMTV ADVQLFVTSA VGGAMVGETV EGIARYPINL RYPQSWRDSP
     QALRQLPILT PMKQQITLAD VADVKVSTGP SMLKTENARP TSWIYIDARD RDMVSVVHDL
     QKAIAEKVQL KPGTSVAFSG QFELLERANH KLKLMVPMTL MIIFVLLYLA FRRVGEALLI
     ISSVPFALVG GIWLLWWMGF HLSVATGTGF IALAGVAAEF GVVMLMYLRH AIEAEPSLNN
     PQTFSEQKLD EALYHGAVLR VRPKAMTVAV IIAGLLPILW GTGAGSEVMS RIAAPMIGGM
     ITAPLLSLFI IPAAYKLMWL HRHRVRK