ID   CUSB_ECO57              Reviewed;         407 AA.
AC   Q8XEH9;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Cation efflux system protein CusB;
DE   Flags: Precursor;
GN   Name=cusB; OrderedLocusNames=Z0713, ECs0612;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Part of a cation efflux system that mediates resistance to
CC       copper and silver. {ECO:0000250}.
CC   -!- SUBUNIT: The cus efflux system is composed of CusA, CusB, CusC and
CC       CusF. {ECO:0000250}.
CC   -!- INDUCTION: Transcriptionally regulated by CusR in response to copper
CC       and silver ions. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG54907.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34035.1; -; Genomic_DNA.
DR   PIR; D90705; D90705.
DR   PIR; G85555; G85555.
DR   RefSeq; NP_308639.1; NC_002695.1.
DR   RefSeq; WP_000717112.1; NZ_LPWC02000002.1.
DR   AlphaFoldDB; Q8XEH9; -.
DR   SMR; Q8XEH9; -.
DR   STRING; 155864.EDL933_0638; -.
DR   PRIDE; Q8XEH9; -.
DR   EnsemblBacteria; AAG54907; AAG54907; Z0713.
DR   EnsemblBacteria; BAB34035; BAB34035; ECs_0612.
DR   GeneID; 916970; -.
DR   KEGG; ece:Z0713; -.
DR   KEGG; ecs:ECs_0612; -.
DR   PATRIC; fig|386585.9.peg.720; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_018816_13_1_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006825; P:copper ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR045800; HMBD.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   Pfam; PF19335; HMBD; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   3: Inferred from homology;
KW   Copper; Copper transport; Ion transport; Reference proteome; Signal;
KW   Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..407
FT                   /note="Cation efflux system protein CusB"
FT                   /id="PRO_0000018691"
FT   CONFLICT        316
FT                   /note="K -> Q (in Ref. 2; BAB34035)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   407 AA;  44323 MW;  B202DFABC28ABC97 CRC64;
     MKKIALIIGS MIAGGIISAA GFTWFAKAEP PAEKTSTAER KILFWYDPMY PNTRFDKPGK
     SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT RGPLTFAQSF PANVSYNEYQ
     YAIVQARAAG FIDKVYPLTV GDKVQKGAPL LDLTIPDWVE AQSEYLLLRE TGGTATQTEG
     ILERLRLAGM PEADIRRLIA TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG
     MDPVWVTAAI PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV
     DNADEALKPG MNAWLKLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF VPKRVAVFQA
     SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS ESATHAH