CUSB_ECOLI
ID CUSB_ECOLI Reviewed; 407 AA.
AC P77239;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Cation efflux system protein CusB;
DE Flags: Precursor;
GN Name=cusB; Synonyms=ylcD; OrderedLocusNames=b0574, JW0563;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP GENE NAME.
RC STRAIN=K12 / DH5-alpha;
RX PubMed=11004187; DOI=10.1128/jb.182.20.5864-5871.2000;
RA Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.;
RT "Identification of a copper-responsive two-component system on the
RT chromosome of Escherichia coli K-12.";
RL J. Bacteriol. 182:5864-5871(2000).
RN [6]
RP FUNCTION IN COPPER HOMEOSTASIS.
RC STRAIN=K12;
RX PubMed=11399769; DOI=10.1074/jbc.m104122200;
RA Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.;
RT "The independent cue and cus systems confer copper tolerance during aerobic
RT and anaerobic growth in Escherichia coli.";
RL J. Biol. Chem. 276:30670-30677(2001).
RN [7]
RP INDUCTION.
RC STRAIN=K38;
RX PubMed=11283292; DOI=10.1099/00221287-147-4-965;
RA Franke S., Grass G., Nies D.H.;
RT "The product of the ybdE gene of the Escherichia coli chromosome is
RT involved in detoxification of silver ions.";
RL Microbiology 147:965-972(2001).
RN [8]
RP FUNCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=12813074; DOI=10.1128/jb.185.13.3804-3812.2003;
RA Franke S., Grass G., Rensing C., Nies D.H.;
RT "Molecular analysis of the copper-transporting efflux system CusCFBA of
RT Escherichia coli.";
RL J. Bacteriol. 185:3804-3812(2003).
CC -!- FUNCTION: Part of a cation efflux system that mediates resistance to
CC copper and silver. {ECO:0000269|PubMed:11399769,
CC ECO:0000269|PubMed:12813074}.
CC -!- SUBUNIT: The cus efflux system is composed of CusA, CusB, CusC and
CC CusF.
CC -!- INTERACTION:
CC P77239; P38054: cusA; NbExp=2; IntAct=EBI-1118842, EBI-1126317;
CC -!- INDUCTION: Transcriptionally regulated by CusR in response to copper
CC and silver ions. {ECO:0000269|PubMed:11283292}.
CC -!- MISCELLANEOUS: The cus system plays an important role in copper
CC tolerance under anaerobic growth and, under extreme copper stress, in
CC aerobic growth.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
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DR EMBL; U82598; AAB40772.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73675.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35208.1; -; Genomic_DNA.
DR PIR; D64790; D64790.
DR RefSeq; NP_415106.1; NC_000913.3.
DR RefSeq; WP_000717157.1; NZ_STEB01000055.1.
DR PDB; 3H94; X-ray; 3.84 A; A/B=1-407.
DR PDB; 3NE5; X-ray; 2.90 A; B/C=1-407.
DR PDB; 3OOC; X-ray; 3.40 A; A/B=1-407.
DR PDB; 3OPO; X-ray; 3.85 A; A/B=1-407.
DR PDB; 3OW7; X-ray; 3.78 A; A/B=1-407.
DR PDB; 3T51; X-ray; 3.90 A; B/C=78-407.
DR PDB; 3T53; X-ray; 3.37 A; B/C=78-407.
DR PDB; 3T56; X-ray; 3.42 A; B/C=78-407.
DR PDB; 4DNR; X-ray; 3.68 A; B/C=1-407.
DR PDB; 4DNT; X-ray; 3.10 A; B/C=1-407.
DR PDB; 4DOP; X-ray; 4.20 A; B/C=1-407.
DR PDBsum; 3H94; -.
DR PDBsum; 3NE5; -.
DR PDBsum; 3OOC; -.
DR PDBsum; 3OPO; -.
DR PDBsum; 3OW7; -.
DR PDBsum; 3T51; -.
DR PDBsum; 3T53; -.
DR PDBsum; 3T56; -.
DR PDBsum; 4DNR; -.
DR PDBsum; 4DNT; -.
DR PDBsum; 4DOP; -.
DR AlphaFoldDB; P77239; -.
DR SMR; P77239; -.
DR BioGRID; 4262993; 137.
DR BioGRID; 849575; 1.
DR ComplexPortal; CPX-2254; Cus cation efflux complex.
DR DIP; DIP-9346N; -.
DR IntAct; P77239; 5.
DR STRING; 511145.b0574; -.
DR TCDB; 2.A.6.1.4; the resistance-nodulation-cell division (rnd) superfamily.
DR PaxDb; P77239; -.
DR PRIDE; P77239; -.
DR EnsemblBacteria; AAC73675; AAC73675; b0574.
DR EnsemblBacteria; BAA35208; BAA35208; BAA35208.
DR GeneID; 945189; -.
DR KEGG; ecj:JW0563; -.
DR KEGG; eco:b0574; -.
DR PATRIC; fig|1411691.4.peg.1700; -.
DR EchoBASE; EB3986; -.
DR eggNOG; COG0845; Bacteria.
DR HOGENOM; CLU_018816_13_1_6; -.
DR InParanoid; P77239; -.
DR OMA; NTHQAIL; -.
DR PhylomeDB; P77239; -.
DR BioCyc; EcoCyc:G6322-MON; -.
DR BioCyc; MetaCyc:G6322-MON; -.
DR EvolutionaryTrace; P77239; -.
DR PRO; PR:P77239; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0030313; C:cell envelope; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0005507; F:copper ion binding; IDA:EcoCyc.
DR GO; GO:0046914; F:transition metal ion binding; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006878; P:cellular copper ion homeostasis; IMP:EcoCyc.
DR GO; GO:0060003; P:copper ion export; IBA:GO_Central.
DR GO; GO:0035434; P:copper ion transmembrane transport; IC:ComplexPortal.
DR GO; GO:0010273; P:detoxification of copper ion; IMP:EcoCyc.
DR GO; GO:0015679; P:plasma membrane copper ion transport; IBA:GO_Central.
DR GO; GO:0046688; P:response to copper ion; IMP:EcoliWiki.
DR GO; GO:0010272; P:response to silver ion; IMP:EcoCyc.
DR GO; GO:0009636; P:response to toxic substance; IC:ComplexPortal.
DR GO; GO:1902601; P:silver ion transmembrane transport; IC:ComplexPortal.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR043602; CusB_dom_1.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR045800; HMBD.
DR InterPro; IPR006143; RND_pump_MFP.
DR Pfam; PF00529; CusB_dom_1; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR Pfam; PF19335; HMBD; 1.
DR TIGRFAMs; TIGR01730; RND_mfp; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Copper; Copper transport; Ion transport; Reference proteome;
KW Signal; Transport.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..407
FT /note="Cation efflux system protein CusB"
FT /id="PRO_0000018690"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 105..124
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 130..135
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:3OOC"
FT STRAND 149..154
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 159..170
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 175..187
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 192..201
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 207..211
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 213..220
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:3T56"
FT STRAND 235..251
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 252..254
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 255..258
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 261..263
FT /evidence="ECO:0007829|PDB:3OOC"
FT STRAND 264..268
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 271..278
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 282..288
FT /evidence="ECO:0007829|PDB:4DNT"
FT TURN 289..292
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 293..301
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 303..305
FT /evidence="ECO:0007829|PDB:3OOC"
FT STRAND 312..320
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 324..328
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 329..331
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 332..334
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 335..337
FT /evidence="ECO:0007829|PDB:3OOC"
FT STRAND 339..344
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 346..348
FT /evidence="ECO:0007829|PDB:3T53"
FT STRAND 350..354
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 356..361
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 364..371
FT /evidence="ECO:0007829|PDB:3NE5"
FT STRAND 376..378
FT /evidence="ECO:0007829|PDB:3NE5"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:3NE5"
FT HELIX 391..396
FT /evidence="ECO:0007829|PDB:3NE5"
SQ SEQUENCE 407 AA; 44305 MW; E949390000C61FC6 CRC64;
MKKIALIIGS MIAGGIISAA GFTWVAKAEP PAEKTSTAER KILFWYDPMY PNTRFDKPGK
SPFMDMDLVP KYADEESSAS GVRIDPTQTQ NLGVKTATVT RGPLTFAQSF PANVSYNEYQ
YAIVQARAAG FIDKVYPLTV GDKVQKGTPL LDLTIPDWVE AQSEYLLLRE TGGTATQTEG
ILERLRLAGM PEADIRRLIA TQKIQTRFTL KAPIDGVITA FDLRAGMNIA KDNVVAKIQG
MDPVWVTAAI PESIAWLVKD ASQFTLTVPA RPDKTLTIRK WTLLPGVDAA TRTLQLRLEV
DNADEALKPG MNAWLQLNTA SEPMLLIPSQ ALIDTGSEQR VITVDADGRF VPKRVAVFQA
SQGVTALRSG LAEGEKVVSS GLFLIDSEAN ISGALERMRS ESATHAH
