CUT1B_ARATH
ID CUT1B_ARATH Reviewed; 174 AA.
AC Q8LCA1; O81038;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Protein CURVATURE THYLAKOID 1B, chloroplastic;
DE AltName: Full=Photosystem I protein P;
DE AltName: Full=Thylakoid membrane phosphoprotein 14 kDa;
DE Flags: Precursor;
GN Name=CURT1B; Synonyms=PSAP, PSI-P, TMP14; OrderedLocusNames=At2g46820;
GN ORFNames=F19D11.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 64-88, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP PHOSPHORYLATION.
RC STRAIN=cv. Wassilewskija; TISSUE=Leaf;
RX PubMed=12883043; DOI=10.1074/mcp.m300050-mcp200;
RA Hansson M., Vener A.V.;
RT "Identification of three previously unknown in vivo protein phosphorylation
RT sites in thylakoid membranes of Arabidopsis thaliana.";
RL Mol. Cell. Proteomics 2:550-559(2003).
RN [6]
RP INTERACTION WITH PSAL, AND PHOSPHORYLATION.
RX PubMed=16109415; DOI=10.1016/j.febslet.2005.07.061;
RA Khrouchtchova A., Hansson M., Paakkarinen V., Vainonen J.P., Zhang S.,
RA Jensen P.E., Scheller H.V., Vener A.V., Aro E.M., Haldrup A.;
RT "A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel
RT subunit of plant photosystem I and is designated PSI-P.";
RL FEBS Lett. 579:4808-4812(2005).
RN [7]
RP INTERACTION WITH PSAL.
RX PubMed=18813226; DOI=10.1038/cr.2008.286;
RA Yu Q.B., Li G., Wang G., Sun J.C., Wang P.C., Wang C., Mi H.L., Ma W.M.,
RA Cui J., Cui Y.L., Chong K., Li Y.X., Li Y.H., Zhao Z., Shi T.L., Yang Z.N.;
RT "Construction of a chloroplast protein interaction network and functional
RT mining of photosynthetic proteins in Arabidopsis thaliana.";
RL Cell Res. 18:1007-1019(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-64, CLEAVAGE OF TRANSIT PEPTIDE
RP [LARGE SCALE ANALYSIS] AFTER ARG-63, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [11]
RP FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION, SUBUNIT, NOMENCLATURE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23839788; DOI=10.1105/tpc.113.113118;
RA Armbruster U., Labs M., Pribil M., Viola S., Xu W., Scharfenberg M.,
RA Hertle A.P., Rojahn U., Jensen P.E., Rappaport F., Joliot P., Doermann P.,
RA Wanner G., Leister D.;
RT "Arabidopsis CURVATURE THYLAKOID1 proteins modify thylakoid architecture by
RT inducing membrane curvature.";
RL Plant Cell 25:2661-2678(2013).
CC -!- FUNCTION: Determines thylakoid architecture by inducing membrane
CC curvature. {ECO:0000269|PubMed:23839788}.
CC -!- SUBUNIT: Homo- and heterodimers and trimers. Interacts with PSAL.
CC {ECO:0000269|PubMed:16109415, ECO:0000269|PubMed:18813226,
CC ECO:0000269|PubMed:23839788}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:23839788}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:23839788}. Note=Located almost exclusively at grana
CC margins. {ECO:0000269|PubMed:23839788}.
CC -!- PTM: Phosphorylated on either Thr-65 or Thr-66 by a threonine specific
CC thylakoid kinase. {ECO:0000269|PubMed:12883043,
CC ECO:0000269|PubMed:16109415}.
CC -!- DISRUPTION PHENOTYPE: No effect on growth behavior, leaf coloration,
CC grana stacks or photochemical efficiency of photosystem II. Curt1a,
CC curt1b, curt1c and curt1d quadruple mutant shows disorganized
CC thylakoids with extended stretches of unstacked membranes and broader
CC stacks made up of fewer layers. {ECO:0000269|PubMed:23839788}.
CC -!- SIMILARITY: Belongs to the CURT family. {ECO:0000305}.
CC -!- CAUTION: Was previously thought to be part of the photosystem I
CC complex. {ECO:0000305|PubMed:16109415}.
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DR EMBL; AC005310; AAC33500.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10758.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10759.1; -; Genomic_DNA.
DR EMBL; AY065156; AAL38332.1; -; mRNA.
DR EMBL; AY114583; AAM47902.1; -; mRNA.
DR EMBL; AY086711; AAM63765.1; -; mRNA.
DR PIR; T02683; T02683.
DR RefSeq; NP_001031551.1; NM_001036474.4.
DR RefSeq; NP_566086.1; NM_130248.5.
DR AlphaFoldDB; Q8LCA1; -.
DR BioGRID; 4630; 2.
DR IntAct; Q8LCA1; 2.
DR MINT; Q8LCA1; -.
DR STRING; 3702.AT2G46820.1; -.
DR TCDB; 8.A.155.1.2; the curt protein (curtp) family.
DR iPTMnet; Q8LCA1; -.
DR PaxDb; Q8LCA1; -.
DR PRIDE; Q8LCA1; -.
DR ProteomicsDB; 220322; -.
DR EnsemblPlants; AT2G46820.1; AT2G46820.1; AT2G46820.
DR EnsemblPlants; AT2G46820.2; AT2G46820.2; AT2G46820.
DR GeneID; 819295; -.
DR Gramene; AT2G46820.1; AT2G46820.1; AT2G46820.
DR Gramene; AT2G46820.2; AT2G46820.2; AT2G46820.
DR KEGG; ath:AT2G46820; -.
DR Araport; AT2G46820; -.
DR TAIR; locus:2044335; AT2G46820.
DR eggNOG; ENOG502QWJR; Eukaryota.
DR HOGENOM; CLU_095488_0_0_1; -.
DR InParanoid; Q8LCA1; -.
DR OMA; NVMGMAT; -.
DR OrthoDB; 1606140at2759; -.
DR PhylomeDB; Q8LCA1; -.
DR PRO; PR:Q8LCA1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LCA1; baseline and differential.
DR Genevisible; Q8LCA1; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0042644; C:chloroplast nucleoid; HDA:TAIR.
DR GO; GO:0030093; C:chloroplast photosystem I; IDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0009515; C:granal stacked thylakoid; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR GO; GO:0090391; P:granum assembly; IMP:TAIR.
DR GO; GO:0097753; P:membrane bending; IDA:TAIR.
DR GO; GO:0009773; P:photosynthetic electron transport in photosystem I; IDA:TAIR.
DR InterPro; IPR025564; CAAD_dom.
DR InterPro; IPR033344; CURT1.
DR PANTHER; PTHR33222; PTHR33222; 1.
DR Pfam; PF14159; CAAD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chloroplast; Direct protein sequencing; Membrane;
KW Phosphoprotein; Plastid; Reference proteome; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..63
FT /note="Chloroplast"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 64..174
FT /note="Protein CURVATURE THYLAKOID 1B, chloroplastic"
FT /id="PRO_0000022558"
FT TOPO_DOM 64..100
FT /note="Stromal"
FT /evidence="ECO:0000269|PubMed:23839788"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..126
FT /note="Lumenal"
FT /evidence="ECO:0000269|PubMed:23839788"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..174
FT /note="Stromal"
FT /evidence="ECO:0000269|PubMed:23839788"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 64
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 110
FT /note="G -> S (in Ref. 4; AAM63765)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="A -> P (in Ref. 4; AAM63765)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 18482 MW; E40E3481BDEC034E CRC64;
MASLSVSSSS TIIDSRAPPS RLASASASSP SCISLPTLPI QSHTRAAKAT AYCRKIVRNV
VTRATTEVGE APATTTEAET TELPEIVKTA QEAWEKVDDK YAIGSLAFAG VVALWGSAGM
ISAIDRLPLV PGVLELVGIG YTGWFTYKNL VFKPDREALF EKVKSTYKDI LGSS
