CUTC_ECOLI
ID CUTC_ECOLI Reviewed; 248 AA.
AC P67826; P46719; P76294;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=PF03932 family protein CutC {ECO:0000255|HAMAP-Rule:MF_00795};
DE AltName: Full=Putative copper homeostasis protein CutC {ECO:0000303|PubMed:7635807};
GN Name=cutC {ECO:0000255|HAMAP-Rule:MF_00795, ECO:0000303|PubMed:7635807};
GN OrderedLocusNames=b1874, JW1863;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RA Gupta S.D., Wu H.C.;
RT "Complete sequence of the E. coli cutC gene.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 44-248, PROBABLE SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=7635807; DOI=10.1128/jb.177.15.4207-4215.1995;
RA Gupta S.D., Lee B.T.O., Camakaris J., Wu H.C.;
RT "Identification of cutC and cutF (nlpE) genes involved in copper tolerance
RT in Escherichia coli.";
RL J. Bacteriol. 177:4207-4215(1995).
RN [6]
RP NOT INVOLVED IN COPPER HOMEOSTASIS, AND DISRUPTION PHENOTYPE.
RX PubMed=25030700; DOI=10.1101/gad.243485.114;
RA Guo M.S., Updegrove T.B., Gogol E.B., Shabalina S.A., Gross C.A., Storz G.;
RT "MicL, a new sigmaE-dependent sRNA, combats envelope stress by repressing
RT synthesis of Lpp, the major outer membrane lipoprotein.";
RL Genes Dev. 28:1620-1634(2014).
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P67825, ECO:0000255|HAMAP-
CC Rule:MF_00795}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00795,
CC ECO:0000305|PubMed:7635807}.
CC -!- DISRUPTION PHENOTYPE: CutC-nlpE double mutants show increased copper
CC sensitivity (PubMed:7635807). However, the copper sensitivity phenotype
CC of the mutant was later shown to be due to the loss of MicL sRNA and
CC elevated Lpp levels (PubMed:25030700). {ECO:0000269|PubMed:25030700,
CC ECO:0000269|PubMed:7635807}.
CC -!- SIMILARITY: Belongs to the CutC family. {ECO:0000255|HAMAP-
CC Rule:MF_00795, ECO:0000305}.
CC -!- CAUTION: Was originally thought to be involved in copper homeostasis
CC (PubMed:7635807), however, the copper sensitivity phenotype of the
CC mutant was later shown to be due to the loss of MicL sRNA which leads
CC to elevated Lpp levels (PubMed:25030700). Silent mutations in this
CC protein that disrupt the promoter of micL are copper sensitive.
CC {ECO:0000269|PubMed:25030700, ECO:0000269|PubMed:7635807}.
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DR EMBL; L38618; AAA89202.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74944.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15684.1; -; Genomic_DNA.
DR PIR; B64950; B64950.
DR RefSeq; WP_001185741.1; NZ_STEB01000026.1.
DR RefSeq; YP_025309.2; NC_000913.3.
DR AlphaFoldDB; P67826; -.
DR SMR; P67826; -.
DR BioGRID; 4262958; 15.
DR IntAct; P67826; 6.
DR STRING; 511145.b1874; -.
DR TCDB; 9.B.158.1.2; the cut copper homeostasis (cut) family.
DR jPOST; P67826; -.
DR PaxDb; P67826; -.
DR PRIDE; P67826; -.
DR EnsemblBacteria; AAC74944; AAC74944; b1874.
DR EnsemblBacteria; BAA15684; BAA15684; BAA15684.
DR GeneID; 2847756; -.
DR GeneID; 58391884; -.
DR KEGG; ecj:JW1863; -.
DR KEGG; eco:b1874; -.
DR PATRIC; fig|1411691.4.peg.374; -.
DR EchoBASE; EB2790; -.
DR eggNOG; COG3142; Bacteria.
DR HOGENOM; CLU_050555_3_1_6; -.
DR InParanoid; P67826; -.
DR OMA; FHRAFDQ; -.
DR PhylomeDB; P67826; -.
DR BioCyc; EcoCyc:G7024-MON; -.
DR PRO; PR:P67826; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005507; F:copper ion binding; IBA:GO_Central.
DR Gene3D; 3.20.20.380; -; 1.
DR HAMAP; MF_00795; CutC; 1.
DR InterPro; IPR005627; Cu_homeostasis_CutC.
DR InterPro; IPR036822; CutC_dom_sf.
DR PANTHER; PTHR12598; PTHR12598; 1.
DR Pfam; PF03932; CutC; 1.
DR SUPFAM; SSF110395; SSF110395; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..248
FT /note="PF03932 family protein CutC"
FT /id="PRO_0000215064"
FT CONFLICT 18
FT /note="Q -> H (in Ref. 1; AAA89202)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 248 AA; 26762 MW; 998A55A417A010B2 CRC64;
MALLEICCYS MECALTAQQN GADRVELCAA PKEGGLTPSL GVLKSVRQRV TIPVHPIIRP
RGGDFCYSDG EFAAILEDVR TVRELGFPGL VTGVLDVDGN VDMPRMEKIM AAAGPLAVTF
HRAFDMCANP LYTLNNLAEL GIARVLTSGQ KSDALQGLSK IMELIAHRDA PIIMAGAGVR
AENLHHFLDA GVLEVHSSAG AWQASPMRYR NQGLSMSSDE HADEYSRYIV DGAAVAEMKG
IIERHQAK
