CUT_DROME
ID CUT_DROME Reviewed; 2175 AA.
AC P10180; Q9W3Q6;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 204.
DE RecName: Full=Homeobox protein cut;
GN Name=ct; ORFNames=CG11387;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2897632; DOI=10.1038/333629a0;
RA Blochlinger K., Bodmer R., Jack J., Jan L.Y., Jan Y.N.;
RT "Primary structure and expression of a product from cut, a locus involved
RT in specifying sensory organ identity in Drosophila.";
RL Nature 333:629-635(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1940 AND SER-1944, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Regulator of cell fate decisions in multiple lineages.
CC Specifically, functions as a determination factor that specifies
CC sensory organ identity in precursor cells. Probably also involved in
CC cell type specification of Malpighian tubules. In absence of cut gene
CC external sensory organs are transformed into chordotonal organs.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Detected in many cells in the central nervous
CC system, all external sensory organs, some peripheral neurons, and in
CC the non-neural cells of the spiracles and the Malpighian tubules.
CC -!- DEVELOPMENTAL STAGE: Cell-specific pattern of expression. Broadly
CC expressed during embryonic development.
CC -!- MISCELLANEOUS: Asn-1791 may participate in regulating DNA-binding
CC activity by promoting homo- and heterodimerization.
CC -!- SIMILARITY: Belongs to the CUT homeobox family. {ECO:0000305}.
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DR EMBL; X07985; CAA30794.1; -; mRNA.
DR EMBL; AE014298; AAF46264.2; -; Genomic_DNA.
DR PIR; S03170; S03170.
DR RefSeq; NP_524764.1; NM_080025.2.
DR AlphaFoldDB; P10180; -.
DR SMR; P10180; -.
DR BioGRID; 69119; 104.
DR DIP; DIP-19788N; -.
DR IntAct; P10180; 10.
DR STRING; 7227.FBpp0113105; -.
DR iPTMnet; P10180; -.
DR PaxDb; P10180; -.
DR DNASU; 44540; -.
DR EnsemblMetazoa; FBtr0071068; FBpp0071026; FBgn0004198.
DR GeneID; 44540; -.
DR KEGG; dme:Dmel_CG11387; -.
DR UCSC; CG11387-RA; d. melanogaster.
DR CTD; 44540; -.
DR FlyBase; FBgn0004198; ct.
DR VEuPathDB; VectorBase:FBgn0004198; -.
DR eggNOG; KOG2252; Eukaryota.
DR GeneTree; ENSGT00940000172657; -.
DR HOGENOM; CLU_001394_1_0_1; -.
DR InParanoid; P10180; -.
DR SignaLink; P10180; -.
DR BioGRID-ORCS; 44540; 1 hit in 3 CRISPR screens.
DR ChiTaRS; ct; fly.
DR GenomeRNAi; 44540; -.
DR PRO; PR:P10180; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004198; Expressed in embryonic/larval spiracle (Drosophila) and 43 other tissues.
DR ExpressionAtlas; P10180; baseline and differential.
DR Genevisible; P10180; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; TAS:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:FlyBase.
DR GO; GO:0007469; P:antennal development; IMP:FlyBase.
DR GO; GO:0048098; P:antennal joint development; IMP:FlyBase.
DR GO; GO:0007417; P:central nervous system development; TAS:FlyBase.
DR GO; GO:0070983; P:dendrite guidance; IMP:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0008585; P:female gonad development; TAS:FlyBase.
DR GO; GO:0060288; P:formation of a compartment boundary; IMP:FlyBase.
DR GO; GO:0008587; P:imaginal disc-derived wing margin morphogenesis; IMP:FlyBase.
DR GO; GO:0061332; P:Malpighian tubule bud morphogenesis; IGI:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; TAS:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; TAS:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; TAS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:FlyBase.
DR GO; GO:0007605; P:sensory perception of sound; IMP:FlyBase.
DR GO; GO:0035277; P:spiracle morphogenesis, open tracheal system; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 3.
DR InterPro; IPR003350; CUT_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR Pfam; PF02376; CUT; 3.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM01109; CUT; 3.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 3.
DR PROSITE; PS51042; CUT; 3.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..2175
FT /note="Homeobox protein cut"
FT /id="PRO_0000202390"
FT DNA_BIND 877..964
FT /note="CUT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00374"
FT DNA_BIND 1330..1417
FT /note="CUT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00374"
FT DNA_BIND 1608..1695
FT /note="CUT 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00374"
FT DNA_BIND 1745..1804
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 139..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 656..765
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1001..1083
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1197..1289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1507..1588
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1695..1747
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1803..1826
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1922..1955
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2069..2097
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2113..2175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 265..343
FT /evidence="ECO:0000255"
FT COILED 433..499
FT /evidence="ECO:0000255"
FT COILED 1056..1161
FT /evidence="ECO:0000255"
FT COILED 1463..1522
FT /evidence="ECO:0000255"
FT COMPBIAS 249..266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 271..298
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..414
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 503..521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 545..560
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..585
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 676..694
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 695..710
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 715..729
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..765
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1008..1028
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1029..1058
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1059..1083
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1209..1224
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1234..1251
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1257..1289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1507..1527
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1569..1583
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1706..1734
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1922..1936
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2138..2165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1940
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1944
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 2175 AA; 233629 MW; 08BF80C4861BD0AB CRC64;
MQPTLPQAAG TADMDLTAVQ SINDWFFKKE QIYLLAQFWQ QRATLAEKEV NTLKEQLSTG
NPDSNLNSEN SDTAAAAATA AAVAAVVAGA TATNDIEDEQ QQQLQQTASG GILESDSDKL
LNSSIVAAAI TLQQQNGSNL LANTNTPSPS PPLLSAEQQQ QLQSSLQQSG GVGGACLNPK
LFFNHAQQMM MMEAAAAAAA AALQQQQQQQ SPLHSPANEV AIPTEQPAAT VATGAAAAAA
AAATPIATGN VKSGSTTSNA NHTNSNNSHQ DEEELDDEEE DEEEDEDEDD EEENASMQSN
ADDMELDAQQ ETRTEPSATT QQQHQQQDTE DLEENKDAGE ASLNVSNNHN TTDSNNSCSR
KNNNGGNESE QHVASSAEDD DCANNNTNTS NNNNTSNTAT SNTNNNNNNN SSSGNSEKRK
KKNNNNNNGQ PAVLLAAKDK EIKALLDELQ RLRAQEQTHL VQIQRLEEHL EVKRQHIIRL
EARLDKQQIN EALAEATALS AAASTNNNNN SQSSDNNKKL NTAAERPMDA SSNADLPEST
KAPVPAEDDE EDEDQAMLVD SEEAEDKPED SHHDDDEDED EDREAVNATT TDSNELKIKK
EQHSPLDLNV LSPNSAIAAA AAAAAAAACA NDPNKFQALL IERTKALAAE ALKNGASDAL
SEDAHHQQQQ HHQQQHQHQQ QHHQQQHLHQ QHHHHLQQQP NSGSNSNPAS NDHHHGHHLH
GHGLLHPSSA HHLHHQTTES NSNSSTPTAA GNNNGSNNSS SNTNANSTAQ LAASLASTLN
GTKSLMQEDS NGLAAVAMAA HAQHAAALGP GFLPGLPAFQ FAAAQVAAGG DGRGHYRFAD
SELQLPPGAS MAGRLGESLI PKGDPMEAKL QEMLRYNMDK YANQALDTLH ISRRVRELLS
VHNIGQRLFA KYILGLSQGT VSELLSKPKP WDKLTEKGRD SYRKMHAWAC DDNAVMLLKS
LIPKKDSGLP QYAGRGAGGA GGDDSMSEDR IAHILSEASS LMKQSSVAQH REQERRSHGG
EDSHSNEDSK SPPQSCTSPF FKVENQLKQH QHLNPEQAAA QQREREREQR EREQQQRLRH
DDQDKMARLY QELIARTPRE TAFPSFLFSP SLFGGAAGMP GAASNAFPAM ADENMRHVFE
REIAKLQQHQ QQQQAAQAQA QFPNFSSLMA LQQQVLNGAQ DLSLAAAAAK DIKLNGQRSS
LEHSAGSSSC SKDGERDDAY PSSLHGRKSE GGGTPAPPAP PSGPGTGAGA PPTAAPPTGG
ASSNSAAPSP LSNSILPPAL SSQGEEFAAT ASPLQRMASI TNSLITQPPV TPHHSTPQRP
TKAVLPPITQ QQFDMFNNLN TEDIVRRVKE ALSQYSISQR LFGESVLGLS QGSVSDLLAR
PKPWHMLTQK GREPFIRMKM FLEDENAVHK LVASQYKIAP EKLMRTGSYS GSPQMPQGLA
SKMQAASLPM QKMMSELKLQ EPAQAQHLMQ QMQAAAMSAA MQQQQVAQAQ QQAQQAQQAQ
QHLQQQAQQH LQQQQHLAQQ QHPHQQHHQA AAAAAALHHQ SMLLTSPGLP PQHAISLPPS
AGGAQPGGPG GNQGSSNPSN SEKKPMLMPV HGTNAMRSLH QHMSPTVYEM AALTQDLDTH
DITTKIKEAL LANNIGQKIF GEAVLGLSQG SVSELLSKPK PWHMLSIKGR EPFIRMQLWL
SDANNVERLQ LLKNERREAS KRRRSTGPNQ QDNSSDTSSN DTNDFYTSSP GPGSVGSGVG
GAPPSKKQRV LFSEEQKEAL RLAFALDPYP NVGTIEFLAN ELGLATRTIT NWFHNHRMRL
KQQVPHGPAG QDNPIPSRES TSATPFDPVQ FRILLQQRLL ELHKERMGMS GAPIPYPPYF
AAAAILGRSL AGIPGAAAAA GAAAAAAAVG ASGGDELQAL NQAFKEQMSG LDLSMPTLKR
ERSDDYQDDL ELEGGGHNLS DNESLEGQEP EDKTTDYEKV LHKSALAAAA AYMSNAVRSS
RRKPAAPQWV NPAGAVTNPS AVVAAVAAAA AAAADNERII NGVCVMQASE YGRDDTDSNK
PTDGGNDSDH EHAQLEIDQR FMEPEVHIKQ EEDDDEEQSG SVNLDNEDNA TSEQKLKVIN
EEKLRMVRVR RLSSTGGGSS EEMPAPLAPP PPPPAASSSI VSGESTTSSS SSSNTSSSTP
AVTTAAATAA AGWNY
