ID   CVAA_ECOLX              Reviewed;         413 AA.
AC   P22519;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 125.
DE   RecName: Full=Colicin V secretion protein CvaA;
GN   Name=cvaA;
OS   Escherichia coli.
OG   Plasmid IncFI ColV3-K30.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFI ColV3-K30;
RX   PubMed=2249654; DOI=10.1002/j.1460-2075.1990.tb07606.x;
RA   Gilson L., Mahanty H.K., Kolter R.;
RT   "Genetic analysis of an MDR-like export system: the secretion of colicin
RT   V.";
RL   EMBO J. 9:3875-3884(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KB101;
RA   Kim Y.N., van Belkum M.J., Stiles M.E.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   TOPOLOGY.
RX   PubMed=7592380; DOI=10.1128/jb.177.21.6153-6159.1995;
RA   Skvirsky R.C., Reginald S., Shen X.;
RT   "Topology analysis of the colicin V export protein CvaA in Escherichia
RT   coli.";
RL   J. Bacteriol. 177:6153-6159(1995).
CC   -!- FUNCTION: Involved, in conjunction with CvaB, in the secretion of
CC       colicin V.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=CvaA;
CC         IsoId=P22519-1; Sequence=Displayed;
CC       Name=CvaA*;
CC         IsoId=P22519-2; Sequence=VSP_018810;
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; X57524; CAA40743.1; -; Genomic_DNA.
DR   EMBL; AY536378; AAS50164.1; -; Genomic_DNA.
DR   PIR; S12271; IKEC5A.
DR   RefSeq; WP_001332356.1; NZ_MW390546.1. [P22519-1]
DR   AlphaFoldDB; P22519; -.
DR   SMR; P22519; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Bacteriocin transport; Cell inner membrane;
KW   Cell membrane; Membrane; Plasmid; Protein transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..413
FT                   /note="Colicin V secretion protein CvaA"
FT                   /id="PRO_0000018693"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:7592380"
FT   TRANSMEM        21..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        37..413
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:7592380"
FT   VAR_SEQ         1..160
FT                   /note="Missing (in isoform CvaA*)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018810"
SQ   SEQUENCE   413 AA;  47344 MW;  E22E85550C84D951 CRC64;
     MKWQGRAILL PGIPLWLIML GSIVFITAFL MFIIVGTYSR RVNVSGEVTT WPRAVNIYSG
     VQGFVVRQFV HEGQLIKKGD PVYLIDISKS TRNGIVTDNH RRDIENQLVR VDNIISRLEE
     SKKITLDTLE KQRLQYTDAF RRSSDIIQRA EEGIKIMKNN MENYRYYQSK GLINKDQLTN
     QVALYYQQQN NLLSLSGQNE QNALQITTLE SQIQTQAADF DNRIYQMELQ RLELQKELVN
     TDVEGEIIIR ALSDGKVDSL SVTVGQMVNT GDSLLQVIPE NIENYYLILW VPNDAVPYIS
     AGDKVNIRYE AFPSEKFGQF SATVKTISRT PASTQEMLTY KGAPQNTPGA SVPWYKVIAT
     PEKQIIRYDE KYLPLENGMK AESTLFLEKR RIYQWMLSPF YDMKHSATGP IND