CVIF1_ARATH
ID CVIF1_ARATH Reviewed; 205 AA.
AC F4HWQ8; Q9C7Y8;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Cell wall / vacuolar inhibitor of fructosidase 1;
DE Short=AtC/VIF1;
DE Flags: Precursor;
GN Name=C/VIF1; Synonyms=CIF1, VIF1; OrderedLocusNames=At1g47960;
GN ORFNames=T2J15.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=14871666; DOI=10.1016/j.bbapap.2003.09.017;
RA Rausch T., Greiner S.;
RT "Plant protein inhibitors of invertases.";
RL Biochim. Biophys. Acta 1696:253-261(2004).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DISULFIDE BOND, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=15327983; DOI=10.1016/j.febslet.2004.07.062;
RA Link M., Rausch T., Greiner S.;
RT "In Arabidopsis thaliana, the invertase inhibitors AtC/VIF1 and 2 exhibit
RT distinct target enzyme specificities and expression profiles.";
RL FEBS Lett. 573:105-109(2004).
CC -!- FUNCTION: Inhibits fructosidases from vacuoles (vacuolar invertase VI).
CC {ECO:0000269|PubMed:14871666, ECO:0000269|PubMed:15327983}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4HWQ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HWQ8-2; Sequence=VSP_043174;
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots, senescent leaves and
CC flowers (in sepals), and, to a lower extent, in stems, specifically in
CC the vascular tissues (e.g. in the phloem).
CC {ECO:0000269|PubMed:15327983}.
CC -!- DISRUPTION PHENOTYPE: Increased vacuolar invertase activity leading to
CC accumulation of hexose. {ECO:0000269|PubMed:15327983}.
CC -!- SIMILARITY: Belongs to the PMEI family. {ECO:0000305}.
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DR EMBL; AC051631; AAG51534.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32232.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58162.1; -; Genomic_DNA.
DR EMBL; AF412119; AAL06571.1; -; mRNA.
DR EMBL; AY056119; AAL07005.1; -; mRNA.
DR EMBL; AY090265; AAL90926.1; -; mRNA.
DR EMBL; BX816333; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY084290; AAM60881.1; -; mRNA.
DR PIR; H96519; H96519.
DR RefSeq; NP_001320618.1; NM_001333288.1. [F4HWQ8-2]
DR RefSeq; NP_564516.2; NM_103692.3. [F4HWQ8-1]
DR AlphaFoldDB; F4HWQ8; -.
DR SMR; F4HWQ8; -.
DR STRING; 3702.AT1G47960.1; -.
DR PaxDb; F4HWQ8; -.
DR PRIDE; F4HWQ8; -.
DR ProteomicsDB; 220323; -. [F4HWQ8-1]
DR EnsemblPlants; AT1G47960.1; AT1G47960.1; AT1G47960. [F4HWQ8-1]
DR EnsemblPlants; AT1G47960.2; AT1G47960.2; AT1G47960. [F4HWQ8-2]
DR GeneID; 841214; -.
DR Gramene; AT1G47960.1; AT1G47960.1; AT1G47960. [F4HWQ8-1]
DR Gramene; AT1G47960.2; AT1G47960.2; AT1G47960. [F4HWQ8-2]
DR KEGG; ath:AT1G47960; -.
DR Araport; AT1G47960; -.
DR TAIR; locus:2202605; AT1G47960.
DR eggNOG; ENOG502S67R; Eukaryota.
DR HOGENOM; CLU_033761_5_0_1; -.
DR InParanoid; F4HWQ8; -.
DR OMA; WIHPLKE; -.
DR OrthoDB; 1302509at2759; -.
DR PRO; PR:F4HWQ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HWQ8; baseline and differential.
DR Genevisible; F4HWQ8; AT.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0004857; F:enzyme inhibitor activity; IDA:UniProtKB.
DR GO; GO:0043086; P:negative regulation of catalytic activity; IDA:UniProtKB.
DR CDD; cd15796; CIF_like; 1.
DR Gene3D; 1.20.140.40; -; 1.
DR InterPro; IPR034087; C/VIF1.
DR InterPro; IPR035513; Invertase/methylesterase_inhib.
DR InterPro; IPR006501; Pectinesterase_inhib_dom.
DR Pfam; PF04043; PMEI; 1.
DR SMART; SM00856; PMEI; 1.
DR SUPFAM; SSF101148; SSF101148; 1.
DR TIGRFAMs; TIGR01614; PME_inhib; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Reference proteome;
KW Signal; Vacuole.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..205
FT /note="Cell wall / vacuolar inhibitor of fructosidase 1"
FT /id="PRO_0000417021"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 30..39
FT /evidence="ECO:0000250"
FT DISULFID 93..134
FT /evidence="ECO:0000250"
FT VAR_SEQ 162..205
FT /note="FYSNSIVKEEACGSSWPSLALNIDSKACVVSLQNIQFNRGRTCW -> VRML
FT L (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.5"
FT /id="VSP_043174"
SQ SEQUENCE 205 AA; 22326 MW; 4BE23211526B24DC CRC64;
MKMMKVMMLI VMMMMVMVMV SEGSIIEPTC KETPDFNLCV SLLNSDPRGS SADTSGLALI
LIDKIKGLAT KTLNEINGLY KKRPELKRAL DECSRRYKTI LNADVPEAIE AISKGVPKFG
EDGVIDAGVE ASVCQGGFNG SSPLTSLTKS MQKISNVTRA IFYSNSIVKE EACGSSWPSL
ALNIDSKACV VSLQNIQFNR GRTCW
