CVPA_ECOLI
ID CVPA_ECOLI Reviewed; 162 AA.
AC P08550;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Colicin V production protein;
DE AltName: Full=Protein DedE;
DE AltName: Full=Pur regulon 18 kDa protein;
GN Name=cvpA; Synonyms=dedE; OrderedLocusNames=b2313, JW2310;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3040734; DOI=10.1016/s0021-9258(18)45338-0;
RA Nonet M.L., Marvel C.C., Tolan D.R.;
RT "The hisT-purF region of the Escherichia coli K-12 chromosome.
RT Identification of additional genes of the hisT and purF operons.";
RL J. Biol. Chem. 262:12209-12217(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2991286; DOI=10.1016/s0021-9258(17)39258-x;
RA Makaroff C.A., Zalkin H.;
RT "Regulation of Escherichia coli purF. Analysis of the control region of a
RT pur regulon gene.";
RL J. Biol. Chem. 260:10378-10387(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP CHARACTERIZATION.
RX PubMed=2542219; DOI=10.1128/jb.171.6.3158-3161.1989;
RA Fath M.J., Mahanty H.K., Kolter R.;
RT "Characterization of a purF operon mutation which affects colicin V
RT production.";
RL J. Bacteriol. 171:3158-3161(1989).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Required for colicin V production from plasmid IncFI ColV3-
CC K30.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
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DR EMBL; J01666; AAA24451.1; -; Genomic_DNA.
DR EMBL; M68934; AAA23968.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75373.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16159.1; -; Genomic_DNA.
DR PIR; A29803; XMECED.
DR RefSeq; NP_416816.1; NC_000913.3.
DR RefSeq; WP_000262111.1; NZ_LN832404.1.
DR AlphaFoldDB; P08550; -.
DR BioGRID; 4260527; 13.
DR DIP; DIP-9354N; -.
DR IntAct; P08550; 1.
DR STRING; 511145.b2313; -.
DR TCDB; 9.B.160.1.16; the colicin v production (cvpa) family.
DR PaxDb; P08550; -.
DR PRIDE; P08550; -.
DR EnsemblBacteria; AAC75373; AAC75373; b2313.
DR EnsemblBacteria; BAA16159; BAA16159; BAA16159.
DR GeneID; 945055; -.
DR KEGG; ecj:JW2310; -.
DR KEGG; eco:b2313; -.
DR PATRIC; fig|1411691.4.peg.4421; -.
DR EchoBASE; EB0167; -.
DR eggNOG; COG1286; Bacteria.
DR HOGENOM; CLU_092720_2_1_6; -.
DR InParanoid; P08550; -.
DR OMA; FTWVDWA; -.
DR PhylomeDB; P08550; -.
DR BioCyc; EcoCyc:EG10169-MON; -.
DR PRO; PR:P08550; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009403; P:toxin biosynthetic process; IMP:EcoCyc.
DR InterPro; IPR003825; Colicin-V_CvpA.
DR Pfam; PF02674; Colicin_V; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..162
FT /note="Colicin V production protein"
FT /id="PRO_0000079581"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..63
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..162
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 162 AA; 17907 MW; 856B4216782A837E CRC64;
MVWIDYAIIA VIAFSSLVSL IRGFVREALS LVTWGCAFFV ASHYYTYLSV WFTGFEDELV
RNGIAIAVLF IATLIVGAIV NFVIGQLVEK TGLSGTDRVL GVCFGALRGV LIVAAILFFL
DSFTGVSKSE DWSKSQLIPQ FSFIIRCFFD YLQSSSSFLP RA
