CV_ARATH
ID CV_ARATH Reviewed; 152 AA.
AC Q8S8K8; Q94CB8;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein CHLOROPLAST VESICULATION {ECO:0000303|PubMed:25538186};
DE Flags: Precursor;
GN Name=CV {ECO:0000303|PubMed:25538186};
GN OrderedLocusNames=At2g25625 {ECO:0000312|Araport:AT2G25625};
GN ORFNames=F3N11 {ECO:0000312|EMBL:AAM15100.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INTERACTION WITH
RP PSBO1, INDUCTION BY SALT AND OXIDATIVE STRESS, REPRESSION BY CYTOKININ,
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=25538186; DOI=10.1105/tpc.114.133116;
RA Wang S., Blumwald E.;
RT "Stress-induced chloroplast degradation in Arabidopsis is regulated via a
RT process independent of autophagy and senescence-associated vacuoles.";
RL Plant Cell 26:4875-4888(2014).
RN [7]
RP INDUCTION BY NAC072/RD26.
RC STRAIN=cv. Columbia;
RX PubMed=29659022; DOI=10.1111/nph.15127;
RA Kamranfar I., Xue G.-P., Tohge T., Sedaghatmehr M., Fernie A.R.,
RA Balazadeh S., Mueller-Roeber B.;
RT "Transcription factor RD26 is a key regulator of metabolic reprogramming
RT during dark-induced senescence.";
RL New Phytol. 218:1543-1557(2018).
CC -!- FUNCTION: Triggers stress-induced chloroplast degradation,
CC independently of autophagy and senescence-associated vacuoles
CC (PubMed:25538186). After targeting to the chloroplast, triggers its
CC destabilization and subsequent disassembly, inducing the formation of
CC CV-containing vesicles (CCVs) carrying stromal proteins, envelope
CC membrane proteins, and thylakoid membrane proteins which are released
CC from the chloroplasts and mobilized to the vacuole for proteolysis
CC (PubMed:25538186). {ECO:0000269|PubMed:25538186}.
CC -!- SUBUNIT: Interacts with the photosystem II subunit PsbO1 via its C-
CC terminal region in the chloroplast thylakoid membrane and in CV-
CC containing vesicles (CCVs). {ECO:0000269|PubMed:25538186}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:25538186}; Single-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:25538186}; Single-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast envelope
CC {ECO:0000269|PubMed:25538186}. Vacuole {ECO:0000269|PubMed:25538186}.
CC Vesicle {ECO:0000269|PubMed:25538186}. Note=Present in vesicle-like
CC spots observed in destabilized chloroplasts; these CV-containing
CC vesicles (CCVs) carrying stromal proteins, envelope membrane proteins,
CC and thylakoid membrane proteins translocate later to the cytosol before
CC being mobilized to the vacuole for proteolysis.
CC {ECO:0000269|PubMed:25538186}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8S8K8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8S8K8-2; Sequence=VSP_061274;
CC -!- TISSUE SPECIFICITY: Mostly expressed in senescent and mature leaves but
CC not in young leaves. {ECO:0000269|PubMed:25538186}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during senescence.
CC {ECO:0000269|PubMed:25538186}.
CC -!- INDUCTION: Induced by abiotic stresses such as salt stress and methyl
CC viologen (MV)-induced oxidative stress (PubMed:25538186). Triggered by
CC NAC072/RD26 during senescence (PubMed:29659022). Down-regulated by
CC cytokinin (PubMed:25538186). {ECO:0000269|PubMed:25538186,
CC ECO:0000269|PubMed:29659022}.
CC -!- DISRUPTION PHENOTYPE: Delayed chloroplast turnover and senescence
CC induced by abiotic stress and associated with an enhanced tolerance to
CC drought, salinity, and oxidative stress. {ECO:0000269|PubMed:25538186}.
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DR EMBL; AC006053; AAM15100.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07726.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07727.1; -; Genomic_DNA.
DR EMBL; AY034981; AAK59486.1; -; mRNA.
DR EMBL; AY063071; AAL34245.1; -; mRNA.
DR EMBL; AK227210; BAE99248.1; -; mRNA.
DR EMBL; AY088242; AAM65783.1; -; mRNA.
DR RefSeq; NP_850063.1; NM_179732.4.
DR AlphaFoldDB; Q8S8K8; -.
DR STRING; 3702.AT2G25625.1; -.
DR PaxDb; Q8S8K8; -.
DR PRIDE; Q8S8K8; -.
DR EnsemblPlants; AT2G25625.1; AT2G25625.1; AT2G25625. [Q8S8K8-1]
DR EnsemblPlants; AT2G25625.2; AT2G25625.2; AT2G25625. [Q8S8K8-2]
DR GeneID; 817103; -.
DR Gramene; AT2G25625.1; AT2G25625.1; AT2G25625. [Q8S8K8-1]
DR Gramene; AT2G25625.2; AT2G25625.2; AT2G25625. [Q8S8K8-2]
DR KEGG; ath:AT2G25625; -.
DR Araport; AT2G25625; -.
DR TAIR; locus:505006272; AT2G25625.
DR eggNOG; ENOG502S9UX; Eukaryota.
DR HOGENOM; CLU_118309_0_0_1; -.
DR InParanoid; Q8S8K8; -.
DR OMA; WRRTEDE; -.
DR OrthoDB; 1501485at2759; -.
DR PhylomeDB; Q8S8K8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8K8; baseline and differential.
DR GO; GO:0009941; C:chloroplast envelope; IDA:UniProtKB.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0097708; C:intracellular vesicle; IDA:TAIR.
DR GO; GO:0005773; C:vacuole; IDA:UniProtKB.
DR GO; GO:1904821; P:chloroplast disassembly; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IEP:UniProtKB.
DR GO; GO:0009735; P:response to cytokinin; IEP:UniProtKB.
DR GO; GO:0006979; P:response to oxidative stress; IMP:UniProtKB.
DR GO; GO:1902074; P:response to salt; IMP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Stress response; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix; Vacuole.
FT TRANSIT 1..22
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 23..152
FT /note="Protein CHLOROPLAST VESICULATION"
FT /id="PRO_0000454278"
FT TRANSMEM 48..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 92..152
FT /note="Important for chloroplast destabilization and the
FT formation of CV-containing vesicles"
FT /evidence="ECO:0000269|PubMed:25538186"
FT VAR_SEQ 34..35
FT /note="CR -> W (in isoform 2)"
FT /id="VSP_061274"
SQ SEQUENCE 152 AA; 16218 MW; 8418841F044BBC38 CRC64;
MAGRISCCLN LPPLDSNSAQ SLASLLKTTS KISCRRTENE TEPRKNKCSF VLGVAATVVI
GGIQINDVAS VEAAVVKSPV EEMAAGVVPP RRWSDKRTCP PWLENSLETI VPENLPRPSA
HRRLELAGLA KGDAPPVGVV MTRVNRGGCF SV
