CWC15_YEAST
ID CWC15_YEAST Reviewed; 175 AA.
AC Q03772; D6VSE3;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Pre-mRNA-splicing factor CWC15;
DE AltName: Full=Complexed with CEF1 protein 15;
GN Name=CWC15; OrderedLocusNames=YDR163W; ORFNames=YD8358.17;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Involved in pre-mRNA splicing.
CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least BUD13, BRR2, CDC40,
CC CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25,
CC CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11,
CC PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2,
CC SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
CC {ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC Q03772; Q03654: CEF1; NbExp=3; IntAct=EBI-36780, EBI-476;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CWC15 family. {ECO:0000305}.
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DR EMBL; Z50046; CAA90383.1; -; Genomic_DNA.
DR EMBL; AY557669; AAS55995.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12003.1; -; Genomic_DNA.
DR PIR; S57987; S57987.
DR RefSeq; NP_010447.3; NM_001180470.3.
DR PDB; 5GM6; EM; 3.50 A; S=1-175.
DR PDB; 5GMK; EM; 3.40 A; S=1-175.
DR PDB; 5LJ3; EM; 3.80 A; P=1-175.
DR PDB; 5MPS; EM; 3.85 A; P=1-175.
DR PDB; 5MQ0; EM; 4.17 A; P=1-175.
DR PDB; 5WSG; EM; 4.00 A; S=1-175.
DR PDB; 5Y88; EM; 3.70 A; P=1-175.
DR PDB; 5YLZ; EM; 3.60 A; P=1-175.
DR PDB; 6BK8; EM; 3.30 A; H=1-175.
DR PDB; 6EXN; EM; 3.70 A; P=1-175.
DR PDB; 6J6G; EM; 3.20 A; S=1-175.
DR PDB; 6J6H; EM; 3.60 A; S=1-175.
DR PDB; 6J6N; EM; 3.86 A; S=1-175.
DR PDB; 6J6Q; EM; 3.70 A; S=1-175.
DR PDB; 7DCO; EM; 2.50 A; S=1-175.
DR PDBsum; 5GM6; -.
DR PDBsum; 5GMK; -.
DR PDBsum; 5LJ3; -.
DR PDBsum; 5MPS; -.
DR PDBsum; 5MQ0; -.
DR PDBsum; 5WSG; -.
DR PDBsum; 5Y88; -.
DR PDBsum; 5YLZ; -.
DR PDBsum; 6BK8; -.
DR PDBsum; 6EXN; -.
DR PDBsum; 6J6G; -.
DR PDBsum; 6J6H; -.
DR PDBsum; 6J6N; -.
DR PDBsum; 6J6Q; -.
DR PDBsum; 7DCO; -.
DR AlphaFoldDB; Q03772; -.
DR SMR; Q03772; -.
DR BioGRID; 32214; 129.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR IntAct; Q03772; 7.
DR STRING; 4932.YDR163W; -.
DR iPTMnet; Q03772; -.
DR MaxQB; Q03772; -.
DR PaxDb; Q03772; -.
DR PRIDE; Q03772; -.
DR EnsemblFungi; YDR163W_mRNA; YDR163W; YDR163W.
DR GeneID; 851741; -.
DR KEGG; sce:YDR163W; -.
DR SGD; S000002570; CWC15.
DR VEuPathDB; FungiDB:YDR163W; -.
DR eggNOG; KOG3228; Eukaryota.
DR HOGENOM; CLU_100667_0_0_1; -.
DR InParanoid; Q03772; -.
DR OMA; NIKEHAT; -.
DR BioCyc; YEAST:G3O-29753-MON; -.
DR PRO; PR:Q03772; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03772; protein.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IGI:SGD.
DR InterPro; IPR006973; Cwf_Cwc_15.
DR PANTHER; PTHR12718; PTHR12718; 2.
DR Pfam; PF04889; Cwf_Cwc_15; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..175
FT /note="Pre-mRNA-splicing factor CWC15"
FT /id="PRO_0000218244"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 85..110
FT /evidence="ECO:0000255"
FT COMPBIAS 53..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 84..114
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..148
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 14..18
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 28..30
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:6BK8"
FT STRAND 131..133
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 160..162
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 164..172
FT /evidence="ECO:0007829|PDB:6J6G"
SQ SEQUENCE 175 AA; 19935 MW; 6F8D838C169F0FF1 CRC64;
MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND
KSLEEAVMNE EKQDVVGSGN LQETRSEKDQ KDSLQELLVT QKNKVEDKAE LEGNEQLKGG
NSSRRSWRKG TAFGRHKVTK ETNIKEHATK KSASGYINDM TKSEYHQEFL HKHVR
