ID   CWC21_CRYNJ             Reviewed;         205 AA.
AC   P0CM94; Q55PK5; Q5KDV1;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Pre-mRNA-splicing factor CWC21;
GN   Name=CWC21; OrderedLocusNames=CNG02690;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. May function at or prior to
CC       the first catalytic step of splicing at the catalytic center of the
CC       spliceosome. May do so by stabilizing the catalytic center or the
CC       position of the RNA substrate (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Associates with the NTC complex (or PRP19-associated complex).
CC       The NTC complex associates with the spliceosome after the release of
CC       the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex
CC       reminiscent of a late-stage spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC21 family. {ECO:0000305}.
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DR   EMBL; AE017347; AAW44625.1; -; Genomic_DNA.
DR   RefSeq; XP_571932.1; XM_571932.1.
DR   AlphaFoldDB; P0CM94; -.
DR   SMR; P0CM94; -.
DR   STRING; 5207.AAW44625; -.
DR   PaxDb; P0CM94; -.
DR   EnsemblFungi; AAW44625; AAW44625; CNG02690.
DR   GeneID; 3258528; -.
DR   KEGG; cne:CNG02690; -.
DR   VEuPathDB; FungiDB:CNG02690; -.
DR   eggNOG; KOG1869; Eukaryota.
DR   HOGENOM; CLU_067891_1_1_1; -.
DR   InParanoid; P0CM94; -.
DR   OMA; LAHMRPR; -.
DR   OrthoDB; 1614776at2759; -.
DR   Proteomes; UP000002149; Chromosome 7.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR   Pfam; PF08312; cwf21; 1.
DR   SMART; SM01115; cwf21; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome.
FT   CHAIN           1..205
FT                   /note="Pre-mRNA-splicing factor CWC21"
FT                   /id="PRO_0000123497"
FT   REGION          143..205
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          63..103
FT                   /evidence="ECO:0000255"
FT   COILED          148..190
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        143..198
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   205 AA;  23856 MW;  BFFA0A28B381E0FF CRC64;
     MYGNVGLATA RGSGTNGYVT RNTAHLRIRE GPPGGQPYGS GYDALLESVS KPPIHRAPDQ
     GILEHERKRR VEVKVMELRD ELEEKGMEED DIEEECSKLR QKLTAQPEQL GGRGLDTHSL
     AAAKEIEMSR LQRALGVSVN HEEGRAFKRE TEEEKAARLA KREERERERI EAAITREREN
     EKRKQEWEEK ERLRRREEYK RRRRD