ID   CWC21_NEUCR             Reviewed;         344 AA.
AC   Q7RYH7;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Pre-mRNA-splicing factor cwc-21;
GN   Name=cwc-21; ORFNames=NCU06498;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. May function at or prior to
CC       the first catalytic step of splicing at the catalytic center of the
CC       spliceosome. May do so by stabilizing the catalytic center or the
CC       position of the RNA substrate (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Associates with the NTC complex (or PRP19-associated complex).
CC       The NTC complex associates with the spliceosome after the release of
CC       the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex
CC       reminiscent of a late-stage spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC21 family. {ECO:0000305}.
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DR   EMBL; CM002238; EAA27902.1; -; Genomic_DNA.
DR   RefSeq; XP_957138.1; XM_952045.2.
DR   AlphaFoldDB; Q7RYH7; -.
DR   SMR; Q7RYH7; -.
DR   STRING; 5141.EFNCRP00000006189; -.
DR   PRIDE; Q7RYH7; -.
DR   EnsemblFungi; EAA27902; EAA27902; NCU06498.
DR   GeneID; 3873276; -.
DR   KEGG; ncr:NCU06498; -.
DR   VEuPathDB; FungiDB:NCU06498; -.
DR   HOGENOM; CLU_067891_0_1_1; -.
DR   InParanoid; Q7RYH7; -.
DR   OMA; RDYQEGS; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR   Pfam; PF08312; cwf21; 1.
DR   SMART; SM01115; cwf21; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome.
FT   CHAIN           1..344
FT                   /note="Pre-mRNA-splicing factor cwc-21"
FT                   /id="PRO_0000123501"
FT   REGION          1..58
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          98..344
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          69..105
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..20
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..58
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        116..164
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..291
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        303..344
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   344 AA;  39836 MW;  CBCFA05DE2184372 CRC64;
     MSDNVGLSTP RGSGTSGYVQ RNLAHFRPRD NYQSYPPKDF DSLKHQPRQP DKGLLEHDRK
     REVEVKVFEL RDKLEEEGVE EDEIETRCDE LRRKLLAEME RNQNSRGAPT GPRKNLKMHQ
     VHELADAKIK ESERLRQALK ISRDYQEGSH WKKQEERLKG ALEREANGDS SSMPPPPAPS
     GPSGGNDRGG DRDRGRGRGF GRRDRDEGRL NSRERRAPPR DWDRPPTPRG RGGRGGRGGR
     DREVDSYRGA AGRDRSRSRS PIRERSRTRS PVRDTGRSRS PVSERSLSRS RSRSRSYSRS
     RSPPRRRAAD SQDRSLSRSR SRSYSRSPDR DRYREKYRDR DNRD