ID   CWC21_TRYB2             Reviewed;         143 AA.
AC   Q38FI2;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Pre-mRNA-splicing factor U5-Cwc21;
GN   ORFNames=Tb09.160.2110;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA   Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA   Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA   Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA   Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA   Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA   Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA   Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA   Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA   Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA   Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA   Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA   Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA   Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA   Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA   Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=19429779; DOI=10.1128/ec.00075-09;
RA   Luz Ambrosio D., Lee J.H., Panigrahi A.K., Nguyen T.N., Cicarelli R.M.,
RA   Gunzl A.;
RT   "Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing
RT   factors.";
RL   Eukaryot. Cell 8:990-1000(2009).
CC   -!- FUNCTION: Essential protein involved in pre-mRNA cis- and trans-
CC       splicing. May function at or prior to the first catalytic step of
CC       splicing at the catalytic center of the spliceosome. May do so by
CC       stabilizing the catalytic center or the position of the RNA substrate.
CC       {ECO:0000269|PubMed:19429779}.
CC   -!- SUBUNIT: Associates with the NTC complex (or PRP19-associated complex)
CC       (By similarity). The NTC complex associates with the spliceosome after
CC       the release of the U1 and U4 snRNAs and forms the CWC spliceosome
CC       subcomplex reminiscent of a late-stage spliceosome (By similarity).
CC       Associates specifically with U5-containing snRNPs. {ECO:0000250,
CC       ECO:0000269|PubMed:19429779}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19429779}. Nucleus
CC       {ECO:0000269|PubMed:19429779}. Note=Found predominantly in the nucleus.
CC   -!- SIMILARITY: Belongs to the CWC21 family. {ECO:0000305}.
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DR   EMBL; CM000207; EAN76438.1; -; Genomic_DNA.
DR   RefSeq; XP_803652.1; XM_798559.1.
DR   AlphaFoldDB; Q38FI2; -.
DR   SMR; Q38FI2; -.
DR   PaxDb; Q38FI2; -.
DR   GeneID; 3660114; -.
DR   KEGG; tbr:Tb09.160.2110; -.
DR   eggNOG; ENOG502S6AD; Eukaryota.
DR   InParanoid; Q38FI2; -.
DR   OMA; RECGELY; -.
DR   Proteomes; UP000008524; Chromosome 9.
DR   GO; GO:0005829; C:cytosol; EXP:GeneDB.
DR   GO; GO:0005634; C:nucleus; IDA:GeneDB.
DR   GO; GO:0097525; C:spliceosomal snRNP complex; IPI:GeneDB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IPI:GeneDB.
DR   GO; GO:0008380; P:RNA splicing; IMP:GeneDB.
DR   InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR   SMART; SM01115; cwf21; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..143
FT                   /note="Pre-mRNA-splicing factor U5-Cwc21"
FT                   /id="PRO_0000403784"
SQ   SEQUENCE   143 AA;  16196 MW;  76FF80DFBCE23028 CRC64;
     MLTTAKANPL EALRSAKENK ELSGQLEHHR SLRAIKLKVL LYREEREAAG VPPDVISREC
     ATLHGSLLRN YMEEAQEARR LGAAELAQKT AERFASAFQV RPGSLGDAFD RRHREVERQA
     AEGARKEAIE RRIVERVKRI KGE