ID   CWC21_USTMA             Reviewed;         348 AA.
AC   Q4P0G6; A0A0D1DNR6;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Pre-mRNA-splicing factor CWC21;
GN   Name=CWC21; ORFNames=UMAG_06397;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in pre-mRNA splicing. May function at or prior to
CC       the first catalytic step of splicing at the catalytic center of the
CC       spliceosome. May do so by stabilizing the catalytic center or the
CC       position of the RNA substrate (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Associates with the NTC complex (or PRP19-associated complex).
CC       The NTC complex associates with the spliceosome after the release of
CC       the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex
CC       reminiscent of a late-stage spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC21 family. {ECO:0000305}.
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DR   EMBL; CM003162; KIS65696.1; -; Genomic_DNA.
DR   RefSeq; XP_011392683.1; XM_011394381.1.
DR   AlphaFoldDB; Q4P0G6; -.
DR   SMR; Q4P0G6; -.
DR   STRING; 5270.UM06397P0; -.
DR   EnsemblFungi; KIS65696; KIS65696; UMAG_06397.
DR   GeneID; 23565998; -.
DR   KEGG; uma:UMAG_06397; -.
DR   VEuPathDB; FungiDB:UMAG_06397; -.
DR   eggNOG; KOG1869; Eukaryota.
DR   HOGENOM; CLU_042840_0_0_1; -.
DR   InParanoid; Q4P0G6; -.
DR   OMA; RDYQEGS; -.
DR   OrthoDB; 1614776at2759; -.
DR   Proteomes; UP000000561; Chromosome 23.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR   Pfam; PF08312; cwf21; 1.
DR   SMART; SM01115; cwf21; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome.
FT   CHAIN           1..348
FT                   /note="Pre-mRNA-splicing factor CWC21"
FT                   /id="PRO_0000123503"
FT   REGION          101..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          168..348
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          81..106
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        173..187
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        199..265
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        266..297
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        298..320
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        334..348
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   348 AA;  38890 MW;  86BC5FFDC65F419B CRC64;
     MYNGIGLKTA RGTGTNGYVQ RNLSNFEAGK KRWNKRDATD EVGSDTRLGP DAAILEHERK
     RAVEVKCMEL RVELEDDDVA DDEIEERVAQ LRETLNAQLA EQTNESAASS YESGRAKETR
     LRHMGTHQQI QTKQHLDRRF AIAVGVESGY RQADAFNRDL QELKKSERIH QQRQAYQQRQ
     LARASHASTQ PPPHPPARCS STHSQSTSSS SPSSPPRKRA RSRSASQDSS ARSSSSHASP
     RSPTPRTTSH VASYSSYSSR SRSRSRSRSP LSHSRSSRSR SRSRSRSRSR SRSPLSHSRS
     SRSRSPSRSR SPSRCASSRS RSPAHRPRRH SSSRVNPPGP PPRRPAGL