CWC22_CHICK
ID CWC22_CHICK Reviewed; 926 AA.
AC Q5ZKA3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Pre-mRNA-splicing factor CWC22 homolog;
DE AltName: Full=Nucampholin homolog;
GN Name=CWC22; Synonyms=NCM; ORFNames=RCJMB04_12b15;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome. Promotes exon-junction complex (EJC) assembly.
CC {ECO:0000250|UniProtKB:Q9HCG8}.
CC -!- SUBUNIT: Component of the pre-catalytic spliceosome B and the catalytic
CC spliceosome C complexes. {ECO:0000250|UniProtKB:Q9HCG8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9HCG8}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q9HCG8}. Note=Concentrates around
CC speckles, which are sites of pre-mRNA synthesis and processing, where
CC it colocalizes with EJC core proteins. {ECO:0000250|UniProtKB:Q9HCG8}.
CC -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAG31840.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ720181; CAG31840.1; ALT_INIT; mRNA.
DR AlphaFoldDB; Q5ZKA3; -.
DR SMR; Q5ZKA3; -.
DR STRING; 9031.ENSGALP00000043058; -.
DR PaxDb; Q5ZKA3; -.
DR PRIDE; Q5ZKA3; -.
DR VEuPathDB; HostDB:geneid_424123; -.
DR eggNOG; KOG2140; Eukaryota.
DR InParanoid; Q5ZKA3; -.
DR PhylomeDB; Q5ZKA3; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR Pfam; PF02847; MA3; 1.
DR SMART; SM00544; MA3; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51366; MI; 1.
PE 2: Evidence at transcript level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..926
FT /note="Pre-mRNA-splicing factor CWC22 homolog"
FT /id="PRO_0000302008"
FT DOMAIN 169..352
FT /note="MIF4G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 457..573
FT /note="MI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 658..926
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..442
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..707
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..856
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 864..917
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 926 AA; 108675 MW; F2C2D28827C2C2B7 CRC64;
MKSRVTQINH GSNHERKERY SPRHRSLTPE DRDVERDRSR SPRRRRYSDD SRYDQEYSRR
EYYDDRSSEG RRMERGRDRY YEKWEERDYD RRRKRRLSSP DHRSPERSTV QGSNTHEEAT
SKKKKEEVDP ILTRTGGAYI PPAKLRMMQE QITDKNSLAY QRMSWEALKK SINGLVNKVN
VSNIENIIHE LLQENIVRGR GLLSRSILQA QGASPIFTHV YAALVAIINS KFPNIGELIL
KRLILNFRKG YRRNDKQLCL TSSKFVAHLM NQNVAHEVLC LEMLTLLLER PTDDSIEVAI
GFLKESGLKL TEVSPRGINA IFDRLRHILH ESKIDMRVQY MIEVMFAVRK DGFKDHPIIP
EGLDLVEEED QFTHMLPLED EYNPEDVLNV FKMDPNFMEN EEKYKALKKE ILDEGDSESE
PDQEAGSSDE EEEEDEEEDE DGQKVTVHDK TEINLVPSVG TIYLAIQSSL DFEECAHKLL
KMDFPESQTK ELCNMILDCC AQQRTYEKFF GLLAGRFCML KKEYMESFEA IFKEQYDTIH
RLETNKLRNV AKMFAHLLYT DSIPWSVLEC IILSEETTTS SSRIFVKIFF QELSEYMGLP
NLNARLKDIT LQPFFEGLLP RDNPRNTRFA INFFTSIGLG GLTDELREHL KNAPKMIMTQ
KQDVESSDSS SSSETDSSSD SDSDSSSSSS ESSSSSDSSS SSSDSSDSDA SKAKKRRTQK
KNRESDKASR KKQEKKRKSL EKKTGRRRQE DRSDSESKSE RNHRNLREAH RRDDTSKYHH
RDESNGRDDY EAYRRDDVSK YHQRDESNGR DNYHSGRDRD HERSKDLENK HSNSKLKKAE
RRASFSDDES YRHGSRDNGH RSRKRERSKS GERSYNKSSP REEEDDHRYR NGSERLREKY
NHYSDQYRES RKHEDRRREN SPHRRK
