ID   CWC22_CRYNB             Reviewed;         831 AA.
AC   P0CM97; Q55Q93; Q5KFX4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Pre-mRNA-splicing factor CWC22;
GN   Name=CWC22; OrderedLocusNames=CNBF4440;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
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DR   EMBL; AAEY01000032; EAL20118.1; -; Genomic_DNA.
DR   RefSeq; XP_774765.1; XM_769672.1.
DR   AlphaFoldDB; P0CM97; -.
DR   SMR; P0CM97; -.
DR   EnsemblFungi; AAW44142; AAW44142; CNF00260.
DR   EnsemblFungi; EAL20118; EAL20118; CNBF4440.
DR   GeneID; 4936996; -.
DR   KEGG; cnb:CNBF4440; -.
DR   VEuPathDB; FungiDB:CNBF4440; -.
DR   HOGENOM; CLU_006308_0_0_1; -.
DR   Proteomes; UP000001435; Chromosome 6.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR   InterPro; IPR003890; MIF4G-like_typ-3.
DR   Pfam; PF02847; MA3; 1.
DR   Pfam; PF02854; MIF4G; 1.
DR   SMART; SM00544; MA3; 1.
DR   SMART; SM00543; MIF4G; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51366; MI; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Spliceosome.
FT   CHAIN           1..831
FT                   /note="Pre-mRNA-splicing factor CWC22"
FT                   /id="PRO_0000410052"
FT   DOMAIN          174..357
FT                   /note="MIF4G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   DOMAIN          460..576
FT                   /note="MI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   REGION          1..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          414..450
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          670..831
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..60
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        422..445
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        670..694
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        695..721
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        735..764
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        780..820
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   831 AA;  94634 MW;  F191FFEFC7BCD5AA CRC64;
     MPRSLSRSVS PRPRSPSLSP PRPDSRSSRG RSLTPDDIPA YKRKRSPSPN PRDRPASPPT
     RRRRSQSPYR NSNRAEIDRP NVKDIDPNRR RARENALLEK QINMALADDG DADGTVATTK
     KSADEIARAE FAKLLGSRSG GAYIPPAKLR AMQAEAAKDK TSAEYQRISW DALKKSINGL
     INKVNISNIK HIVPELFGEN LIRGRGLFAR SVMRAQASSL PFTPVFAALV AIINTKLPQV
     GELVLIRLIS QFRRAYKRND KTVCHATSTF IAHLCNQYVA HEIVALQILL LCLDRPTDDS
     IEVAVGFMRE VGQFLSENSP KANNTVFERF RAVLHEGQIS KRCQYMIEVL FQVRKDKYKD
     NPAVPEGLDL VEEEEQITHR VTLDDELQVQ ESLNLFKADP NFVQNEERYN AIKREILGDS
     DDESGTESGS EYSESEDDED EDVAPEKAGI QDMTETNLIN LRRTIYLTIM NSLNFEEAVH
     KLMKVNIPEG REIELCNMVI ECCSQERTYS NFYGLIGERF CKLHRIWTDA FQEAFQKYYD
     TIHRYETNKL RNIGRFFGHL LASDGISWAV LHVVHMNEEE TTSSSRIFVK IVLQEMVEEM
     GINRVAERFR IPDLKPAFAG MFPMDNPKNA RFSINYFTSI GMGKVTEDMR EYLQNAPKLL
     AAQQAALAAA ESSDSDTDSS SDISSSSDSD SDTDSDASSY SRRSRRRRYS SDSRSPPPQR
     RRYSSDSRSP SPPPRRRQYS DEPRSPSPPP RRRRYSDDSR SPSPPPRRRY SGDSRSPSPP
     PRRRYADDSR SPSPPPHRRR YSDDSRSPSP PPRRRRYSDN SRSPSPPPRR R