ID   CWC22_MUSDO             Reviewed;        1292 AA.
AC   A0A1I8M2I8;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   18-JAN-2017, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Pre-mRNA-splicing factor CWC22 homolog {ECO:0000305};
DE   AltName: Full=Nucampholin {ECO:0000303|PubMed:28495751};
GN   Name=ncm {ECO:0000303|PubMed:28495751};
OS   Musca domestica (House fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC   Muscidae; Musca.
OX   NCBI_TaxID=7370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=aabys;
RX   PubMed=25315136; DOI=10.1186/s13059-014-0466-3;
RA   Scott J.G., Warren W.C., Beukeboom L.W., Bopp D., Clark A.G., Giers S.D.,
RA   Hediger M., Jones A.K., Kasai S., Leichter C.A., Li M., Meisel R.P.,
RA   Minx P., Murphy T.D., Nelson D.R., Reid W.R., Rinkevich F.D.,
RA   Robertson H.M., Sackton T.B., Sattelle D.B., Thibaud-Nissen F.,
RA   Tomlinson C., van de Zande L., Walden K.K., Wilson R.K., Liu N.;
RT   "Genome of the house fly, Musca domestica L., a global vector of diseases
RT   with adaptations to a septic environment.";
RL   Genome Biol. 15:RESEARCH0466.1-RESEARCH0466.16(2014).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=28495751; DOI=10.1126/science.aam5498;
RA   Sharma A., Heinze S.D., Wu Y., Kohlbrenner T., Morilla I., Brunner C.,
RA   Wimmer E.A., van de Zande L., Robinson M.D., Beukeboom L.W., Bopp D.;
RT   "Male sex in houseflies is determined by Mdmd, a paralog of the generic
RT   splice factor gene CWC22.";
RL   Science 356:642-645(2017).
CC   -!- FUNCTION: Required for pre-mRNA splicing and for exon-junction complex
CC       (EJC) assembly. Hinders eIF4AIII from non-specifically binding RNA and
CC       escorts it to the splicing machinery to promote EJC assembly on mature
CC       mRNAs. {ECO:0000250|UniProtKB:Q9HCG8}.
CC   -!- SUBUNIT: Component of the spliceosome C complex.
CC       {ECO:0000250|UniProtKB:Q9HCG8}.
CC   -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q9HCG8}.
CC   -!- DISRUPTION PHENOTYPE: Early lethality in both males and females.
CC       {ECO:0000269|PubMed:28495751}.
CC   -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
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DR   AlphaFoldDB; A0A1I8M2I8; -.
DR   SMR; A0A1I8M2I8; -.
DR   STRING; 7370.XP_005185085.1; -.
DR   VEuPathDB; VectorBase:MDOA000598; -.
DR   eggNOG; KOG2140; Eukaryota.
DR   Proteomes; UP000095301; Unplaced.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR   InterPro; IPR003890; MIF4G-like_typ-3.
DR   Pfam; PF02847; MA3; 1.
DR   SMART; SM00544; MA3; 1.
DR   SMART; SM00543; MIF4G; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51366; MI; 1.
PE   3: Inferred from homology;
KW   mRNA processing; mRNA splicing; Nucleus.
FT   CHAIN           1..1292
FT                   /note="Pre-mRNA-splicing factor CWC22 homolog"
FT                   /id="PRO_0000441320"
FT   DOMAIN          483..666
FT                   /note="MIF4G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   DOMAIN          776..892
FT                   /note="MI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   REGION          1..425
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          726..767
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          989..1292
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..21
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        35..53
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        59..89
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..149
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        150..175
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        176..200
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        204..265
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        266..287
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        288..315
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        316..355
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        356..425
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        729..744
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        989..1031
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1042..1082
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1083..1097
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1133..1292
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1292 AA;  147084 MW;  158618C7CB06D797 CRC64;
     MTGSQSENNT STSSNSSEDT NNDSRNESET NADKNVVETK TTSDNNNKMM NAAETAANEE
     KNGRQKKEKS KTPSKEDKKS RKKKSESSSE SSSSSDSDSS ESSSSSSDGE VSTSSGSSSD
     SEKVKSKVKN KSKSPSAQRE VVQKETVTDT PDNISKETQE KLVEDIPKEN ELNVNLAKED
     SVNAKQNDTE ASNENVAEEI TKPPQKQPDT EEGEITENNE KNSSARSPSK EKQLSANRSR
     SRERRSHSGD SRGAHSGDKG SPSRLKRSPS RSKKSPSRSK RSVSRNRSSS RHGRDSSRNR
     RSVSGDKENQ LRRKSRSRSS RSRSRSRSRS RSRRPERKHR SRTPRRSRSR ERRHERRRSV
     SSDYDARRRS RRSESIERRR EERKRRHAER DEREKSKRSR RDEDDSFKTN KVSAEMEKDK
     ENDNATVTDP KAKITERQRK TVDILTSRTG GAYIPPAKLR MMQAEITDKA SAAYQRIAWE
     ALKKSIHGYI NKVNVDNIAI ITRELLKENI VRGRGLLCRS IIQAQAASPT FTHVYAALVA
     IINSKFPNIG ELLLKRLVIQ FKRAFRRNDK TVCLSSSRFI AHLVNQRVAH EILALEILTL
     LVESPTDDSV EVAIAFLKEC GMKLTEVSSK GIGAIFEMLK NILHEGKLDK RVQYMIEVVF
     QVRKDGFKDH QSVIESLELV EEDDQFTHLL MLDDATSPED VLNAFKFDEQ YEANEEKYKG
     LSKEILGSDA SDSDGSSGSG SDSDSESSDS DEDKNEGDEK PTAGDIIDNT ETNLIALRRT
     IYLTINSSLD YEECAHKLMK MQLKPGQEIE LCHMFLDCCA EQRTYEKFYG LLAQRFCNIN
     KSYIEPFEEI FKDTYQTTHR LDTNRLRNVS KFFAHLLFTD AISWDVLDCI KLNEDDTTSS
     SRIFIKILFQ ELAEYMGLGQ LNKKLKDEVL AESLAGLFPK DNPRNTRFSI NFFTSIGLGG
     LTDELRQFLK NAPKSVPAIN AEILANKPVD VSNSSSSSSS SSSSTSSSSS SSSSSSESSA
     SSSSSDSSSD SSSDSEVDKK KRKGKVKKTK KKKTSNKKEK TKKSKSKNKK DAKKKAKKRK
     SKKGSTSSED DSSKSESSSS ESKSSDSSDS EQSGNDKSKK KTKSKSKTKP NKKSKRSDSE
     DVENERDIKR KKREDFGNYI HEDRRRDEEY SKNGGRDRKK DNHGNNKEKR EIPQRRDSEI
     ERRREEREKR HRERERNFSR SRSRSRGGSG RKDRDRRETN GRSERKDRER DRDSDHRRYR
     KDYDRSRSRD RNEKREREYS RSKLRNTSKE RG