ID   CWC22_NEUCR             Reviewed;        1010 AA.
AC   Q7RX84;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Pre-mRNA-splicing factor cwc22;
DE   AltName: Full=mRNA-splicing protein-1;
GN   Name=msp-1; Synonyms=cwc22; ORFNames=NCU00066;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
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DR   EMBL; CM002238; EAA27140.3; -; Genomic_DNA.
DR   RefSeq; XP_956376.3; XM_951283.3.
DR   AlphaFoldDB; Q7RX84; -.
DR   SMR; Q7RX84; -.
DR   STRING; 5141.EFNCRP00000000380; -.
DR   EnsemblFungi; EAA27140; EAA27140; NCU00066.
DR   GeneID; 3872537; -.
DR   KEGG; ncr:NCU00066; -.
DR   VEuPathDB; FungiDB:NCU00066; -.
DR   HOGENOM; CLU_006308_0_2_1; -.
DR   InParanoid; Q7RX84; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR   InterPro; IPR003890; MIF4G-like_typ-3.
DR   Pfam; PF02847; MA3; 1.
DR   Pfam; PF02854; MIF4G; 1.
DR   SMART; SM00544; MA3; 1.
DR   SMART; SM00543; MIF4G; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51366; MI; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..1010
FT                   /note="Pre-mRNA-splicing factor cwc22"
FT                   /id="PRO_0000215674"
FT   DOMAIN          222..405
FT                   /note="MIF4G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   DOMAIN          507..623
FT                   /note="MI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   REGION          1..166
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          466..498
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          708..1010
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        13..28
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        43..59
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..82
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        83..112
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        142..156
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        467..487
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        715..735
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        736..750
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        751..767
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        768..786
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        963..980
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        981..1002
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1010 AA;  114000 MW;  24FB817AC1419F19 CRC64;
     MASADMSPSR SHPHDATRSP SPRTQSPSPR DEDGSRSPGE RTPSPPSRDP SPYRSPGERT
     PSPSPRRDRS LSPRDQPHSH PRSRSPTPRS QSPSRRSVRS PSPRQGSPAR RVDRSSSPRA
     RSPPPRRHSR SPPLRGQPPP PRHRDAGGDY RPVRKERTPT PPPVAVKTEE EKLADARAEY
     QKLLNLRSQG VYLPPHRLRA LQAAITDKKT REYQRMAWEA LKKSVNGLVN KVNTANIKFV
     VPELFGENLI RGRGLFCQSL LKAQHASLPF TPIYACLAAI CNTKLPQVGE LLVKRLVLRF
     RKAFKRNDKA VCLSSTMFIA HLVNNQVVHE MIAAQILLLL LAKPTDDSVE IAVGLMREVG
     LFLEEMSPAI AHAVFDQFRN ILHEADIDRR TQYMIEVLFQ VRKDKYKDNP VIKEELDLVE
     EEDQITHRIG LDDEIDPQDG LNVFKMDPNW EENEEEYKKL KAEILGEASD DDEDDDDDDE
     SESGSESEDE EQKALEIKDQ SNADLVNLRR TIYLSIQSSA DPEEAAHKLM KLRLPAGQEA
     ELVSMIVESC AQEKVYLKFM GLLGERFARL NRMWMDLFEE SFAKYYSTIH RYETNKLRNI
     ARFFGHLLAT DAIGWHVFSV IHLNEEETTS ASRIFIKILF EDLQENIGSA KLKARMSEET
     LQPSLQGIFP HDEPRNIRFS INYFTSIKMG YLTDEMRTFL ANMPKPALPA PPADSDSESV
     SSYSSYSSYS SRSRSRSLTP RKDTRGRSLS RTPPRRGRGR SYSRTPSRSR SRSRSYSRSV
     SKSVSRSPPR RRAVESRSPS PPPRGRGRSY DRYSRSPSRS RSRTRSPAAA PPIRRGRSGT
     RSRSRSYSRS PSPPPARGYP TRGRAPVSNN DRAAAASGKR RREGSYSASR SPHPPPQQRL
     RRGSYSRSRS RSPIPIRGNG PAGRDTGRAG PAPARGGRRN RSYSRSRTRS PPPLADAATG
     SRRVVSRSPS PVVGNNKRRR SYSSSRSRSR SSSRSRYRSR SPVAKRGRVD