ID   CWC23_KLULA             Reviewed;         270 AA.
AC   Q6CUS9;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Pre-mRNA-splicing factor CWC23;
GN   Name=CWC23; OrderedLocusNames=KLLA0C02541g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. May be involved in endoplasmic
CC       reticulum-associated protein degradation (ERAD) and required for growth
CC       at low and high temperatures (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
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DR   EMBL; CR382123; CAH01161.1; -; Genomic_DNA.
DR   RefSeq; XP_452310.1; XM_452310.1.
DR   AlphaFoldDB; Q6CUS9; -.
DR   SMR; Q6CUS9; -.
DR   STRING; 28985.XP_452310.1; -.
DR   EnsemblFungi; CAH01161; CAH01161; KLLA0_C02541g.
DR   GeneID; 2892427; -.
DR   KEGG; kla:KLLA0_C02541g; -.
DR   eggNOG; KOG0716; Eukaryota.
DR   HOGENOM; CLU_063717_0_0_1; -.
DR   InParanoid; Q6CUS9; -.
DR   OMA; KHFKLPY; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   Pfam; PF00226; DnaJ; 1.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome.
FT   CHAIN           1..270
FT                   /note="Pre-mRNA-splicing factor CWC23"
FT                   /id="PRO_0000071127"
FT   DOMAIN          12..84
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ   SEQUENCE   270 AA;  32374 MW;  492BDA42F18A06E4 CRC64;
     MDLAHVKDNH VDLYRILHIH VNDNEHLNSV TPTLINKQFR KLSLTLHPDK SNESDLNITR
     DRWDNLQSAY RILSEHKKEY DIWYQRTFLH SNRELLEKLE ISEKAKKEKL ISYDEIEKIQ
     RYGQTLRKLV HFEIPISDWR NPSFTENTDD LNKLTETCLF RIKLVKRKEY NNEQKLNEWF
     RKIDIPITVQ YYSENNDQRE DDLVVYASAR NVQTTIEILR TIENEKELHP DILEFTPAVE
     FEHFSFKEKV ELDPSLGSII YNSSDNPIIM