CWC23_YEAST
ID CWC23_YEAST Reviewed; 283 AA.
AC P52868; D6VU20; Q6B1S2;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Pre-mRNA-splicing factor CWC23;
DE AltName: Full=Complexed with CEF1 protein 23;
GN Name=CWC23; OrderedLocusNames=YGL128C; ORFNames=G2861;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8686378;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<145::aid-yea888>3.0.co;2-e;
RA Rodriguez-Belmonte E., Rodriguez Torres A.M., Tizon B., Cadahia J.L.,
RA Gonzalez-Siso I., Ramil E., Becerra M., Gonzalez-Dominguez M., Cerdan E.;
RT "Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII
RT reveals a novel member of the DnaJ family.";
RL Yeast 12:145-148(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=10029993;
RX DOI=10.1002/(sici)1097-0061(19990130)15:2<145::aid-yea346>3.0.co;2-j;
RA Tizon B., Rodriguez-Torres A.M., Cerdan M.E.;
RT "Disruption of six novel Saccharomyces cerevisiae genes reveals that
RT YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c
RT for growth at low or high temperatures and YGL125w is implicated in the
RT biosynthesis of methionine.";
RL Yeast 15:145-154(1999).
RN [6]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
RN [7]
RP FUNCTION.
RX PubMed=12847107; DOI=10.1074/jbc.m301080200;
RA Taxis C., Hitt R., Park S.-H., Deak P.M., Kostova Z., Wolf D.H.;
RT "Use of modular substrates demonstrates mechanistic diversity and reveals
RT differences in chaperone requirement of ERAD.";
RL J. Biol. Chem. 278:35903-35913(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [9]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [10]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [11]
RP FUNCTION.
RX PubMed=15170475; DOI=10.1038/sj.embor.7400172;
RA Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.;
RT "The J-protein family: modulating protein assembly, disassembly and
RT translocation.";
RL EMBO Rep. 5:567-571(2004).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in pre-mRNA splicing. May be involved in endoplasmic
CC reticulum-associated protein degradation (ERAD) and required for growth
CC at low and high temperatures. {ECO:0000269|PubMed:10029993,
CC ECO:0000269|PubMed:12847107, ECO:0000269|PubMed:15170475}.
CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2,
CC CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9,
CC PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
CC {ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC P52868; Q06411: SPP382; NbExp=6; IntAct=EBI-560, EBI-576;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT93027.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA60703.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA96837.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X87252; CAA60703.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z72650; CAA96837.1; ALT_INIT; Genomic_DNA.
DR EMBL; AY693008; AAT93027.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006941; DAA07981.1; -; Genomic_DNA.
DR PIR; S64139; S64139.
DR RefSeq; NP_011387.2; NM_001180993.1.
DR PDB; 5Y88; EM; 3.70 A; T=1-283.
DR PDBsum; 5Y88; -.
DR AlphaFoldDB; P52868; -.
DR SMR; P52868; -.
DR BioGRID; 33123; 32.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR DIP; DIP-6831N; -.
DR IntAct; P52868; 17.
DR MINT; P52868; -.
DR STRING; 4932.YGL128C; -.
DR MaxQB; P52868; -.
DR PaxDb; P52868; -.
DR PRIDE; P52868; -.
DR TopDownProteomics; P52868; -.
DR EnsemblFungi; YGL128C_mRNA; YGL128C; YGL128C.
DR GeneID; 852749; -.
DR KEGG; sce:YGL128C; -.
DR SGD; S000003096; CWC23.
DR VEuPathDB; FungiDB:YGL128C; -.
DR eggNOG; KOG0716; Eukaryota.
DR HOGENOM; CLU_063717_0_0_1; -.
DR InParanoid; P52868; -.
DR OMA; KHFKLPY; -.
DR BioCyc; YEAST:G3O-30624-MON; -.
DR PRO; PR:P52868; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P52868; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IDA:SGD.
DR GO; GO:0000390; P:spliceosomal complex disassembly; IGI:SGD.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..283
FT /note="Pre-mRNA-splicing factor CWC23"
FT /id="PRO_0000071129"
FT DOMAIN 15..87
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ SEQUENCE 283 AA; 33237 MW; AA1D0894328CAEA5 CRC64;
MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH PDKHPDNPSI
IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN KLIQKLEESE SSTIPTTTPH
PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN TQDQENASQH PYYDCSTLRI VLDNFLQSNN
KSNCLSHLRN QVFITLSANE IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG
NLIPTVKDIS PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD
