ID   CWC24_ASHGO             Reviewed;         250 AA.
AC   Q752S4;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Pre-mRNA-splicing factor CWC24;
GN   Name=CWC24; OrderedLocusNames=AFR499C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC24 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53870.1; -; Genomic_DNA.
DR   RefSeq; NP_986046.1; NM_212182.1.
DR   AlphaFoldDB; Q752S4; -.
DR   SMR; Q752S4; -.
DR   STRING; 33169.AAS53870; -.
DR   PRIDE; Q752S4; -.
DR   EnsemblFungi; AAS53870; AAS53870; AGOS_AFR499C.
DR   GeneID; 4622325; -.
DR   KEGG; ago:AGOS_AFR499C; -.
DR   eggNOG; KOG1813; Eukaryota.
DR   HOGENOM; CLU_050460_3_0_1; -.
DR   InParanoid; Q752S4; -.
DR   OMA; LHIRDEM; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000349; P:generation of catalytic spliceosome for first transesterification step; IEA:EnsemblFungi.
DR   GO; GO:0034247; P:snoRNA splicing; IBA:GO_Central.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR039971; CWC24-like.
DR   InterPro; IPR000571; Znf_CCCH.
DR   InterPro; IPR036855; Znf_CCCH_sf.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR12930; PTHR12930; 1.
DR   Pfam; PF00642; zf-CCCH; 1.
DR   SMART; SM00184; RING; 1.
DR   SMART; SM00356; ZnF_C3H1; 1.
DR   SUPFAM; SSF90229; SSF90229; 1.
DR   PROSITE; PS50103; ZF_C3H1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome; Zinc; Zinc-finger.
FT   CHAIN           1..250
FT                   /note="Pre-mRNA-splicing factor CWC24"
FT                   /id="PRO_0000055883"
FT   ZN_FING         101..129
FT                   /note="C3H1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT   ZN_FING         158..196
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT   REGION          44..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          213..250
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        226..250
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   250 AA;  27355 MW;  6E7DC4303CD3F869 CRC64;
     MFRKRNVKGS QQKKAALRVV EDDVECLATP PVVPAKKRKI RELDDTSRSA VRASGFGRDH
     VSAGTQEAVA RATDDAPDRR AESSGARTAV GSNVRTRVVM DYQPDVCKDY RQTGFCGYGD
     SCKFLHSRDD FRAGWRLNEE WKVGQTEARD LDSIPFRCVL CRGHYRAPVR TRCGHYFCGG
     CFARRVRETR QCAVCGADTQ GVAQSAARLR ELLAAGAGAG DSPDDTPAEE EPRSPGEPAA
     GEERRGAESG