CWC25_MOUSE
ID CWC25_MOUSE Reviewed; 416 AA.
AC Q9DBF7; A2A6F4; Q6AXH2; Q7TSF8;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Pre-mRNA-splicing factor CWC25 homolog;
DE AltName: Full=Coiled-coil domain-containing protein 49;
DE AltName: Full=Spliceosome-associated protein homolog CWC25;
GN Name=Cwc25; Synonyms=Ccdc49;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the
CC spliceosome. {ECO:0000250|UniProtKB:Q9NXE8}.
CC -!- SUBUNIT: Identified in the spliceosome C complex.
CC {ECO:0000250|UniProtKB:Q9NXE8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NXE8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9DBF7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DBF7-2; Sequence=VSP_025798;
CC Name=3;
CC IsoId=Q9DBF7-3; Sequence=VSP_025799;
CC -!- SIMILARITY: Belongs to the CWC25 family. {ECO:0000305}.
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DR EMBL; AK004983; BAB23718.1; -; mRNA.
DR EMBL; AL596446; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC053447; AAH53447.1; -; mRNA.
DR EMBL; BC079553; AAH79553.1; -; mRNA.
DR CCDS; CCDS25330.1; -. [Q9DBF7-1]
DR RefSeq; NP_080462.2; NM_026186.4. [Q9DBF7-1]
DR AlphaFoldDB; Q9DBF7; -.
DR BioGRID; 212219; 2.
DR IntAct; Q9DBF7; 1.
DR STRING; 10090.ENSMUSP00000018685; -.
DR iPTMnet; Q9DBF7; -.
DR PhosphoSitePlus; Q9DBF7; -.
DR EPD; Q9DBF7; -.
DR jPOST; Q9DBF7; -.
DR MaxQB; Q9DBF7; -.
DR PaxDb; Q9DBF7; -.
DR PeptideAtlas; Q9DBF7; -.
DR PRIDE; Q9DBF7; -.
DR ProteomicsDB; 279304; -. [Q9DBF7-1]
DR ProteomicsDB; 279305; -. [Q9DBF7-2]
DR ProteomicsDB; 279306; -. [Q9DBF7-3]
DR Antibodypedia; 74912; 27 antibodies from 12 providers.
DR DNASU; 67480; -.
DR Ensembl; ENSMUST00000018685; ENSMUSP00000018685; ENSMUSG00000018541. [Q9DBF7-1]
DR GeneID; 67480; -.
DR KEGG; mmu:67480; -.
DR UCSC; uc007ler.2; mouse. [Q9DBF7-1]
DR CTD; 54883; -.
DR MGI; MGI:1914730; Cwc25.
DR VEuPathDB; HostDB:ENSMUSG00000018541; -.
DR eggNOG; KOG3869; Eukaryota.
DR GeneTree; ENSGT00440000039055; -.
DR HOGENOM; CLU_025093_1_0_1; -.
DR InParanoid; Q9DBF7; -.
DR OMA; SWHPHTM; -.
DR OrthoDB; 1635950at2759; -.
DR PhylomeDB; Q9DBF7; -.
DR TreeFam; TF320801; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 67480; 10 hits in 70 CRISPR screens.
DR ChiTaRS; Cwc25; mouse.
DR PRO; PR:Q9DBF7; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9DBF7; protein.
DR Bgee; ENSMUSG00000018541; Expressed in manus and 212 other tissues.
DR ExpressionAtlas; Q9DBF7; baseline and differential.
DR Genevisible; Q9DBF7; MM.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR InterPro; IPR019339; CIR_N_dom.
DR InterPro; IPR022209; CWC25.
DR Pfam; PF10197; Cir_N; 1.
DR Pfam; PF12542; CWC25; 1.
DR SMART; SM01083; Cir_N; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Isopeptide bond; mRNA processing;
KW mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome;
KW Ubl conjugation.
FT CHAIN 1..416
FT /note="Pre-mRNA-splicing factor CWC25 homolog"
FT /id="PRO_0000288866"
FT REGION 164..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 20..70
FT /evidence="ECO:0000255"
FT COILED 324..413
FT /evidence="ECO:0000255"
FT COMPBIAS 176..194
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..280
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..296
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..343
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 170
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXE8"
FT MOD_RES 222
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXE8"
FT CROSSLNK 382
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NXE8"
FT VAR_SEQ 1..338
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025798"
FT VAR_SEQ 326..416
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025799"
FT CONFLICT 301
FT /note="N -> D (in Ref. 1; BAB23718)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 48811 MW; 67AC732FED303C72 CRC64;
MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV
GAVKKKEEKL DWMYQGPGGM VNRDEYLLGR PIDKYVFEKM EEREAGCSSE TGLLPGSIFA
PSGANSLLDM ASKIREDPLF IIRKKEEEKK REVLNNPVKM KKIKELLQMS LEKKEKKKKK
EKKKKHRKHK HRSSSSGGSS SEDEQSQARS QKKMANSFPV LSKVPGYGLQ VRDSDRNRGL
QGSLGEQRAI KNNSRSRSSS PPRHASKKST KEERPRDRRS RSPSRRSRSP RPSKPHTSKV
NRKERDSPSP KKEAYQRRHA SGYTRKLSAE ELERKRQEMM ENAKWREEER LNTLKRHARE
DEREHRLERL DSRSGKFLHR MKLESASTSS LEDRVKRNIH SLQRTSVALE KNFMRR
