ID   CWF11_SCHPO             Reviewed;        1284 AA.
AC   O94508;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=Pre-mRNA-splicing factor cwf11;
DE   AltName: Full=Complexed with cdc5 protein 11;
GN   Name=cwf11; ORFNames=SPBC646.02;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA   Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT   "Proteomics analysis reveals stable multiprotein complexes in both fission
RT   and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT   splicing factors, and snRNAs.";
RL   Mol. Cell. Biol. 22:2011-2024(2002).
CC   -!- FUNCTION: Involved in mRNA splicing where it associates with cdc5 and
CC       the other cwf proteins as part of the spliceosome.
CC   -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC       spliceosome sub-complex reminiscent of a late-stage spliceosome
CC       composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC       cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC       cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC       cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC       cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC       prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC       smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC   -!- INTERACTION:
CC       O94508; P39964: cdc5; NbExp=3; IntAct=EBI-539118, EBI-538771;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CWF11 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA22806.1; -; Genomic_DNA.
DR   PIR; T40578; T40578.
DR   RefSeq; NP_595360.1; NM_001021268.2.
DR   PDB; 3JB9; EM; 3.60 A; X=1-1284.
DR   PDBsum; 3JB9; -.
DR   AlphaFoldDB; O94508; -.
DR   SMR; O94508; -.
DR   BioGRID; 277639; 47.
DR   IntAct; O94508; 7.
DR   STRING; 4896.SPBC646.02.1; -.
DR   MaxQB; O94508; -.
DR   PaxDb; O94508; -.
DR   PRIDE; O94508; -.
DR   EnsemblFungi; SPBC646.02.1; SPBC646.02.1:pep; SPBC646.02.
DR   GeneID; 2541124; -.
DR   KEGG; spo:SPBC646.02; -.
DR   PomBase; SPBC646.02; cwf11.
DR   VEuPathDB; FungiDB:SPBC646.02; -.
DR   eggNOG; KOG1806; Eukaryota.
DR   HOGENOM; CLU_001195_0_0_1; -.
DR   InParanoid; O94508; -.
DR   OMA; YQFIDVP; -.
DR   PhylomeDB; O94508; -.
DR   Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR   Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   PRO; PR:O94508; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IDA:PomBase.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IC:PomBase.
DR   CDD; cd18808; SF1_C_Upf1; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR032174; Aquarius_N.
DR   InterPro; IPR026300; CWF11_fam.
DR   InterPro; IPR045055; DNA2/NAM7-like.
DR   InterPro; IPR041679; DNA2/NAM7-like_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10887; PTHR10887; 2.
DR   PANTHER; PTHR10887:SF5; PTHR10887:SF5; 2.
DR   Pfam; PF13087; AAA_12; 1.
DR   Pfam; PF16399; Aquarius_N; 1.
DR   PIRSF; PIRSF038901; AQR_cwf11; 1.
PE   1: Evidence at protein level;
KW   3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome.
FT   CHAIN           1..1284
FT                   /note="Pre-mRNA-splicing factor cwf11"
FT                   /id="PRO_0000079611"
SQ   SEQUENCE   1284 AA;  148372 MW;  F751A9F4B36534D5 CRC64;
     MVLYKNKKKQ IINYANSNWG QNEGVEFQET MLQCILEAVI VSKDAKQVLS LLHELKLFEN
     FLWQRVNTEM SLNHINLTCM LLLYKSKYEY ITWDLIDENR FQLFFEKVIE VSLSLNLSEV
     VYMIQFITLC FQFSNIEKLR KLVYQLTNIS ILNSLDNLDK VKYLLHDSSS LTKAFDSYKE
     KRPSIVEKFP LHNLLSRWIH SLLIKSISYA QTEKQEAKVT PLLAIINMSL VLLSAFPTRR
     FAHPVIEDSC FYTALRMSLY YDSNELFKKM TDDLNYVLKF PFDNTRGNEY EKEQKIRNDE
     LVYYHLQLTL FSDFQKELGD LVFCTQTSLQ QRQKLEEITS FLSFNSLKSL CSKCYLRTSF
     PEKYAIKVDF EFLKNVFINT YDRTRLVNDY DEIINFTLKD VLGERSVMDQ ENSLTNYFLL
     QNTAIQYLSI SFFMRQQSKA YKKLLLRSLY AELLNFSEQY RRLSIKNATK NLTKDNFFSL
     NNFKVTSVAP PQIGQVLPQF VKCQMGLSRP GPFHSALRDL KNSIKSPFLC LIYISKDMEY
     KLLHGNALDP LEGVTDFTIA TICNDDVGMF QSDMQSDSDN KSINVYLSPF YYHSLAGLGE
     YRPKQLKFNF ALVLSPEANK YWLDLNILVS LLNRAKEFPK WFEDLFLGFG TPDICAFPNA
     GLNSIYARNL FNTVEQLQSV LPNCHVPSNL STESLLIKFY TNQNKISADV TASDRHFLLP
     SNRLYTYNDK QLESILRGSQ PGLTMVNGPT RCGKHVLVCK LLEVLQDTSP NDRTVVLSDS
     NFSMNTLFTL LEKARCFHQG HLLYLSDEGK DETLERYGTL SSWISKLPGL LREIGRLAAS
     IQAPGSHDAS PDTALYFRDA YIKRLWEKYL NTVDDKDSVD AYNRFPFHSY FGDKSKRPIE
     TYNKDNFFDY ATKLYGELEY MFQQLEEIRP FGLLRYYEDQ ELYALCQQSR IIGCTWTSLS
     TRLGTLKEKG FCFNNLIVMN SQNISESSIT SILLSNCEPT GFDRLVLLGN QYLTSGNQDI
     NNTSNGSLFK RLRYLKSRII DLNTQYNVRE SISSLCSSIY PLDIKTVDSS PNKRLDYGNS
     GFAHEVQFIN VGAFKGSQET EPVSGYKQNL GEAEYAVALF QYMRMLGYPT NEIVICTLYE
     SQVSLLNEII SVRCSHNSFF GQPAFVGTVE KLPSDKRVNF VIFTTVESKE ASDHWNPKTF
     YKAFSACSYG LYVLCNRDLF RSTRGLEKLW NEIEKTPDKL LLTTGEIYPS SHKIGSSVET
     FEIENLLHLS NYVVEMTKKR LNTN