CWF17_SCHPO
ID CWF17_SCHPO Reviewed; 340 AA.
AC O94620;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Pre-mRNA-splicing factor cwf17;
DE AltName: Full=Complexed with cdc5 protein 17;
GN Name=cwf17; ORFNames=SPBC1289.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Involved in mRNA splicing where it associates with cdc5 and
CC the other cwf proteins as part of the spliceosome.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC O94620; P39964: cdc5; NbExp=3; IntAct=EBI-539124, EBI-538771;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; CU329671; CAB38691.2; -; Genomic_DNA.
DR PIR; T39362; T39362.
DR RefSeq; NP_596835.1; NM_001023856.2.
DR PDB; 3JB9; EM; 3.60 A; L=1-340.
DR PDBsum; 3JB9; -.
DR AlphaFoldDB; O94620; -.
DR SMR; O94620; -.
DR BioGRID; 276528; 50.
DR IntAct; O94620; 8.
DR STRING; 4896.SPBC1289.11.1; -.
DR MaxQB; O94620; -.
DR PaxDb; O94620; -.
DR PRIDE; O94620; -.
DR EnsemblFungi; SPBC1289.11.1; SPBC1289.11.1:pep; SPBC1289.11.
DR GeneID; 2539984; -.
DR KEGG; spo:SPBC1289.11; -.
DR PomBase; SPBC1289.11; -.
DR VEuPathDB; FungiDB:SPBC1289.11; -.
DR eggNOG; KOG0265; Eukaryota.
DR HOGENOM; CLU_000288_57_2_1; -.
DR InParanoid; O94620; -.
DR OMA; WDLRKNA; -.
DR PhylomeDB; O94620; -.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:O94620; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR GO; GO:0005682; C:U5 snRNP; IDA:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IC:PomBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..340
FT /note="Pre-mRNA-splicing factor cwf17"
FT /id="PRO_0000050951"
FT REPEAT 48..87
FT /note="WD 1"
FT REPEAT 91..130
FT /note="WD 2"
FT REPEAT 133..173
FT /note="WD 3"
FT REPEAT 175..214
FT /note="WD 4"
FT REPEAT 217..256
FT /note="WD 5"
FT REPEAT 267..306
FT /note="WD 6"
FT REPEAT 308..339
FT /note="WD 7"
SQ SEQUENCE 340 AA; 37427 MW; BBAA5938CA4C24C2 CRC64;
MDKRKESESA TNGHLVKRIR IQDSSLITEG SVLQRTSDLN VPNLQMFGHT AEVLVARFDP
SGSYFASGGM DRQILLWNVF GDVKNYGVLN GCKGAITDLQ WSRDSRVVYC SSSDTHLMSW
DAVSGQKIRK HKGHAGVVNA LDVLKVGSEL LTSVSDDCTM KVWDSRSKDC IKTIEEKYPL
TAVAIAQQGT QVFIGGIDGA IKIWDLRNNH CSHVLKGHKD IITSLAISKD GSSLLSNSMD
NTVRIFDVKP FASAQRQLQI FEGAIHGQEH NLLGVAWSRN SRFVGAGSSD KNVYVWSATG
DLRYVLPGHE GSVNHVDFHP HQDIILSCSS DRTIFLGELN
