CWF19_SCHPO
ID CWF19_SCHPO Reviewed; 639 AA.
AC Q09909;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Pre-mRNA-splicing factor cwf19;
DE AltName: Full=Complexed with cdc5 protein 19;
GN Name=cwf19; ORFNames=SPAC30D11.09;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Involved in mRNA splicing where it associates with cdc5 and
CC the other cwf proteins as part of the spliceosome.
CC -!- FUNCTION: Involved in mRNA splicing.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CWF19 family. {ECO:0000305}.
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DR EMBL; CU329670; CAA91895.1; -; Genomic_DNA.
DR PIR; T38590; S62567.
DR RefSeq; NP_593208.1; NM_001018604.2.
DR PDB; 3JB9; EM; 3.60 A; c=1-639.
DR PDBsum; 3JB9; -.
DR AlphaFoldDB; Q09909; -.
DR SMR; Q09909; -.
DR BioGRID; 279618; 101.
DR IntAct; Q09909; 4.
DR STRING; 4896.SPAC30D11.09.1; -.
DR iPTMnet; Q09909; -.
DR MaxQB; Q09909; -.
DR PaxDb; Q09909; -.
DR PRIDE; Q09909; -.
DR EnsemblFungi; SPAC30D11.09.1; SPAC30D11.09.1:pep; SPAC30D11.09.
DR GeneID; 2543189; -.
DR KEGG; spo:SPAC30D11.09; -.
DR PomBase; SPAC30D11.09; cwf19.
DR VEuPathDB; FungiDB:SPAC30D11.09; -.
DR eggNOG; KOG2477; Eukaryota.
DR HOGENOM; CLU_015540_2_1_1; -.
DR InParanoid; Q09909; -.
DR OMA; VPGHCRI; -.
DR PhylomeDB; Q09909; -.
DR PRO; PR:Q09909; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IDA:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IC:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.30.428.10; -; 1.
DR InterPro; IPR040194; Cwf19-like.
DR InterPro; IPR006768; Cwf19-like_C_dom-1.
DR InterPro; IPR006767; Cwf19-like_C_dom-2.
DR InterPro; IPR036265; HIT-like_sf.
DR PANTHER; PTHR12072; PTHR12072; 2.
DR Pfam; PF04677; CwfJ_C_1; 1.
DR Pfam; PF04676; CwfJ_C_2; 1.
DR SUPFAM; SSF54197; SSF54197; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Spliceosome.
FT CHAIN 1..639
FT /note="Pre-mRNA-splicing factor cwf19"
FT /id="PRO_0000079614"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 86..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..39
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 639 AA; 74420 MW; 95F79E570802DA1B CRC64;
MEKEKLTNRT STHKFNETDR ETNHSRRHRH HHSRHRESKH GRHDRSERPS SREAGDRSRH
ERFSKEPLEV KKLPVENEYF DSKFASASSM PSSKDNPQNN DFTNSLQGLD FGTFGSRIQR
EPIDRKSNKP GEIFVGATLQ NESSTSTEHK DIEQLETEIN ESGINYKIGD NGSRWRMMKL
KRVFDMAQDQ RRPVEEVALE RYGSLKEFDF ALEERDELEL RKRTRKVQKE VPTGELYEKR
CADEFKTRKD SGNGATYYKV IPDPSVNKDT PTESDLNRLK ADLLRAQLKK DPNYESLERE
YQKACDDFEN SLHPSENSNS QATIPSKKRN FDDPTIDEMV QEEKLLNKQR KYGQNYEYAK
QIAKDKTYSN NLDYLDENAG KLSNRMKRGD VSLAQISSGG DLKKINHVLD TCPLCLNYET
QPLAPVISLS HRAYVSLPTQ PELAKYHCLI VPTGHRINTL SCDEDEWDEI RNFMKCIALM
FDSMNLGVIF YENAPSPQRY MHTAIECIPV SKRILSLAPA YFREALSTSD EEWSQHRKII
DTLEGSKKYG KWAFRKMMVK ELGYFHVWFS IDGGYGHVVE DEKAWGRHDQ VPRQVFASML
NLPPEVIRRK GSWTGKKDPR EDMFRSRFEK FDWTKGLID
