ID   CWLJ_BACSU              Reviewed;         142 AA.
AC   P42249;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Cell wall hydrolase CwlJ;
GN   Name=cwlJ; Synonyms=ycbQ; OrderedLocusNames=BSU02600;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA   Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT   "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT   region of the Bacillus subtilis chromosome.";
RL   Microbiology 141:269-275(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 86.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   CHARACTERIZATION.
RC   STRAIN=168;
RX   PubMed=9515903; DOI=10.1128/jb.180.6.1375-1380.1998;
RA   Ishikawa S., Yamane K., Sekiguchi J.;
RT   "Regulation and characterization of a newly deduced cell wall hydrolase
RT   gene (cwlJ) which affects germination of Bacillus subtilis spores.";
RL   J. Bacteriol. 180:1375-1380(1998).
RN   [5]
RP   ACTIVITY REGULATION.
RC   STRAIN=168 / PS832;
RX   PubMed=11466292; DOI=10.1128/jb.183.16.4886-4893.2001;
RA   Paidhungat M., Ragkousi K., Setlow P.;
RT   "Genetic requirements for induction of germination of spores of Bacillus
RT   subtilis by Ca(2+)-dipicolinate.";
RL   J. Bacteriol. 183:4886-4893(2001).
RN   [6]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=11807087; DOI=10.1128/jb.184.4.1219-1224.2002;
RA   Bagyan I., Setlow P.;
RT   "Localization of the cortex lytic enzyme CwlJ in spores of Bacillus
RT   subtilis.";
RL   J. Bacteriol. 184:1219-1224(2002).
RN   [7]
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=168;
RX   PubMed=12177332; DOI=10.1099/00221287-148-8-2383;
RA   Chirakkal H., O'Rourke M., Atrih A., Foster S.J., Moir A.;
RT   "Analysis of spore cortex lytic enzymes and related proteins in Bacillus
RT   subtilis endospore germination.";
RL   Microbiology 148:2383-2392(2002).
CC   -!- FUNCTION: Probable spore cortex-lytic enzyme involved in the
CC       depolymerization of cortex peptidoglycan during germination.
CC   -!- ACTIVITY REGULATION: Activated, directly or indirectly, by endogenous
CC       spore dipicolinic acid released in response to nutrients. Is also
CC       activated by exogenous Ca(2+)-dipicolinic acid.
CC       {ECO:0000269|PubMed:11466292}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during sporulation; in mother cell
CC       compartment, then located in the spore coat.
CC       {ECO:0000269|PubMed:11807087, ECO:0000269|PubMed:12177332}.
CC   -!- INDUCTION: Expression is sigma E-dependent.
CC   -!- SIMILARITY: Belongs to the CwlJ family. {ECO:0000305}.
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DR   EMBL; D30808; BAA06481.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12054.2; -; Genomic_DNA.
DR   PIR; H69610; H69610.
DR   RefSeq; NP_388142.2; NC_000964.3.
DR   RefSeq; WP_003246500.1; NZ_JNCM01000030.1.
DR   AlphaFoldDB; P42249; -.
DR   SMR; P42249; -.
DR   IntAct; P42249; 3.
DR   STRING; 224308.BSU02600; -.
DR   PaxDb; P42249; -.
DR   PRIDE; P42249; -.
DR   EnsemblBacteria; CAB12054; CAB12054; BSU_02600.
DR   GeneID; 938399; -.
DR   KEGG; bsu:BSU02600; -.
DR   PATRIC; fig|224308.179.peg.269; -.
DR   eggNOG; COG3773; Bacteria.
DR   OMA; MIYQPHA; -.
DR   PhylomeDB; P42249; -.
DR   BioCyc; BSUB:BSU02600-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.2520; -; 1.
DR   InterPro; IPR011105; Cell_wall_hydrolase_SleB.
DR   InterPro; IPR042047; SleB_dom1.
DR   Pfam; PF07486; Hydrolase_2; 1.
PE   1: Evidence at protein level;
KW   Cell wall biogenesis/degradation; Germination; Hydrolase;
KW   Reference proteome; Secreted; Sporulation.
FT   CHAIN           1..142
FT                   /note="Cell wall hydrolase CwlJ"
FT                   /id="PRO_0000164426"
FT   CONFLICT        86
FT                   /note="R -> G (in Ref. 1; BAA06481)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   142 AA;  16463 MW;  324F5BE4E3970912 CRC64;
     MAVVRATSAD VDLMARLLRA EAEGEGKQGM LLVGNVGINR LRANCSDFKG LRTIRQMIYQ
     PHAFEAVTHG YFYQRARDSE RALARRSING ERRWPAKFSL WYFRPQGDCP AQWYNQPFVA
     RFKSHCFYQP TAETCENVYN TF