ID   CWSA_MYCS2              Reviewed;         136 AA.
AC   A0QNF5;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Cell wall synthesis protein CwsA {ECO:0000255|HAMAP-Rule:MF_00927};
DE   AltName: Full=Cell wall synthesis and cell shape protein A {ECO:0000255|HAMAP-Rule:MF_00927};
GN   Name=cwsA {ECO:0000255|HAMAP-Rule:MF_00927};
GN   OrderedLocusNames=MSMEG_0023, MSMEI_0025;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, INTERACTION WITH CRGA AND WAG31, SUBCELLULAR LOCATION, DISRUPTION
RP   PHENOTYPE, AND GENE NAME.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=23002219; DOI=10.1128/jb.01005-12;
RA   Plocinski P., Arora N., Sarva K., Blaszczyk E., Qin H., Das N.,
RA   Plocinska R., Ziolkiewicz M., Dziadek J., Kiran M., Gorla P., Cross T.A.,
RA   Madiraju M., Rajagopalan M.;
RT   "Mycobacterium tuberculosis CwsA interacts with CrgA and Wag31, and the
RT   CrgA-CwsA complex is involved in peptidoglycan synthesis and cell shape
RT   determination.";
RL   J. Bacteriol. 194:6398-6409(2012).
CC   -!- FUNCTION: Required for regulated cell division, cell wall synthesis and
CC       the maintenance of cell shape. {ECO:0000255|HAMAP-Rule:MF_00927,
CC       ECO:0000269|PubMed:23002219}.
CC   -!- SUBUNIT: Interacts with CrgA and Wag31. {ECO:0000269|PubMed:23002219}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:23002219};
CC       Single-pass membrane protein {ECO:0000305|PubMed:23002219}.
CC       Note=Localizes to poles and midcell sites.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to defects in cell shape and polar
CC       peptidoglycan synthesis. It also compromises polar localization of
CC       Wag31. {ECO:0000269|PubMed:23002219}.
CC   -!- SIMILARITY: Belongs to the CwsA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00927}.
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DR   EMBL; CP000480; ABK75126.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36508.1; -; Genomic_DNA.
DR   RefSeq; WP_011726615.1; NZ_SIJM01000001.1.
DR   RefSeq; YP_884443.1; NC_008596.1.
DR   AlphaFoldDB; A0QNF5; -.
DR   STRING; 246196.MSMEI_0025; -.
DR   PRIDE; A0QNF5; -.
DR   EnsemblBacteria; ABK75126; ABK75126; MSMEG_0023.
DR   EnsemblBacteria; AFP36508; AFP36508; MSMEI_0025.
DR   GeneID; 66738214; -.
DR   KEGG; msg:MSMEI_0025; -.
DR   KEGG; msm:MSMEG_0023; -.
DR   PATRIC; fig|246196.19.peg.21; -.
DR   eggNOG; ENOG5030K8D; Bacteria.
DR   OMA; KRPWVIA; -.
DR   OrthoDB; 1949735at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR   GO; GO:0042546; P:cell wall biogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0008104; P:protein localization; IMP:CACAO.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00927; CwsA; 1.
DR   InterPro; IPR024245; CwsA.
DR   Pfam; PF10814; CwsA; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cell membrane; Cell shape; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..136
FT                   /note="Cell wall synthesis protein CwsA"
FT                   /id="PRO_0000421170"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00927"
SQ   SEQUENCE   136 AA;  14401 MW;  65F425B9CC4B17B0 CRC64;
     MPARADVRLA PRQRLTRGLK YTAVGPVDIT RGVLGIGADT AQATAAELRR RYASGKLQRQ
     LAAAAEAVAA LPETIQEAVQ EVVSPPKKRR RRPLLIAAVA VTVLGGGAAA FSIVRRRSRP
     QEPPTLAPSV EVAPKP