ID   CWSA_MYCTU              Reviewed;         145 AA.
AC   P9WJF3; L0T2A0; P71577;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Cell wall synthesis protein CwsA {ECO:0000255|HAMAP-Rule:MF_00927};
DE   AltName: Full=Cell wall synthesis and cell shape protein A {ECO:0000255|HAMAP-Rule:MF_00927};
GN   Name=cwsA {ECO:0000255|HAMAP-Rule:MF_00927}; OrderedLocusNames=Rv0008c;
GN   ORFNames=MTCY10H4.07c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION, INTERACTION WITH CRGA AND WAG31, SUBCELLULAR LOCATION, AND GENE
RP   NAME.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=23002219; DOI=10.1128/jb.01005-12;
RA   Plocinski P., Arora N., Sarva K., Blaszczyk E., Qin H., Das N.,
RA   Plocinska R., Ziolkiewicz M., Dziadek J., Kiran M., Gorla P., Cross T.A.,
RA   Madiraju M., Rajagopalan M.;
RT   "Mycobacterium tuberculosis CwsA interacts with CrgA and Wag31, and the
RT   CrgA-CwsA complex is involved in peptidoglycan synthesis and cell shape
RT   determination.";
RL   J. Bacteriol. 194:6398-6409(2012).
CC   -!- FUNCTION: Required for regulated cell division, cell wall synthesis and
CC       the maintenance of cell shape. {ECO:0000255|HAMAP-Rule:MF_00927,
CC       ECO:0000269|PubMed:23002219}.
CC   -!- SUBUNIT: Interacts with CrgA and Wag31. {ECO:0000269|PubMed:23002219}.
CC   -!- INTERACTION:
CC       P9WJF3; P9WP57: crgA; NbExp=3; IntAct=EBI-6420113, EBI-6414478;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:23002219};
CC       Single-pass membrane protein {ECO:0000305|PubMed:23002219}.
CC       Note=Localizes to poles and midcell sites.
CC   -!- SIMILARITY: Belongs to the CwsA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00927}.
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DR   EMBL; AL123456; CCP42730.1; -; Genomic_DNA.
DR   PIR; F70698; F70698.
DR   RefSeq; NP_214522.1; NC_000962.3.
DR   RefSeq; WP_003917266.1; NZ_NVQJ01000005.1.
DR   AlphaFoldDB; P9WJF3; -.
DR   BMRB; P9WJF3; -.
DR   IntAct; P9WJF3; 2.
DR   STRING; 83332.Rv0008c; -.
DR   PaxDb; P9WJF3; -.
DR   DNASU; 887085; -.
DR   GeneID; 887085; -.
DR   KEGG; mtu:Rv0008c; -.
DR   TubercuList; Rv0008c; -.
DR   eggNOG; ENOG5030K8D; Bacteria.
DR   OMA; KRPWVIA; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR   GO; GO:0042546; P:cell wall biogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00927; CwsA; 1.
DR   InterPro; IPR024245; CwsA.
DR   Pfam; PF10814; CwsA; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cell membrane; Cell shape; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..145
FT                   /note="Cell wall synthesis protein CwsA"
FT                   /id="PRO_0000103640"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00927"
SQ   SEQUENCE   145 AA;  15671 MW;  7C17CCD1189707D7 CRC64;
     MSEQVETRLT PRERLTRGLA YSAVGPVDVT RGLLELGVGL GLQSARSTAA GLRRRYREGR
     LAREVAAAQE TLAQELTAAQ DVVANLPQAL QDARTQRRSK HHLWIFAGIA AAILAGGAVA
     FSIVRRSSRP EPSPRPPSVE VQPRS