CWZF3_ORYSJ
ID CWZF3_ORYSJ Reviewed; 902 AA.
AC Q0DRX6; A0A0P0VY74; Q10LI9;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2020, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Cysteine-tryptophan domain-containing zinc finger protein 3 {ECO:0000303|PubMed:28818372};
DE Short=OsCW-ZF3 {ECO:0000303|PubMed:28818372};
GN Name=CWZF3 {ECO:0000303|PubMed:28818372};
GN OrderedLocusNames=Os03g0347300 {ECO:0000312|EMBL:BAF12012.1},
GN LOC_Os03g22580 {ECO:0000312|EMBL:ABF95911.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN, AND MUTAGENESIS
RP OF TRP-27; TRP-36 AND TRP-52.
RX PubMed=28818372; DOI=10.1016/j.plantsci.2017.06.013;
RA Zhang Z., Zhang F., Cheng Z.J., Liu L.L., Lin Q.B., Wu F.Q., Zhang H.,
RA Wang J.L., Wang J., Guo X.P., Zhang X., Lei C.L., Zhao Z.C., Zhu S.S.,
RA Wan J.M.;
RT "Functional characterization of rice CW-domain containing zinc finger
RT proteins involved in histone recognition.";
RL Plant Sci. 263:168-176(2017).
CC -!- FUNCTION: Binds to histones H3K4me1, H3K4me2 and H3K4me3 in GST pull-
CC down assay (PubMed:28818372). May facilitate the recruitment of
CC effectors to mediate gene expression (By similarity).
CC {ECO:0000250|UniProtKB:A0A0P0X9Z7, ECO:0000269|PubMed:28818372}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28818372}.
CC -!- TISSUE SPECIFICITY: Expressed in leaf sheaths, flag leaves, nodes,
CC internodes and panicles. {ECO:0000269|PubMed:28818372}.
CC -!- DOMAIN: The CW-type zinc finger domain is not required for nuclear
CC localization. {ECO:0000269|PubMed:28818372}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAS84173.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DP000009; ABF95911.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12012.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84173.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q0DRX6; -.
DR SMR; Q0DRX6; -.
DR STRING; 4530.OS03T0347300-01; -.
DR EnsemblPlants; Os03t0347300-01; Os03t0347300-01; Os03g0347300.
DR Gramene; Os03t0347300-01; Os03t0347300-01; Os03g0347300.
DR eggNOG; ENOG502SC6K; Eukaryota.
DR HOGENOM; CLU_014232_0_0_1; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR011124; Znf_CW.
DR Pfam; PF07496; zf-CW; 1.
DR PROSITE; PS51050; ZF_CW; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..902
FT /note="Cysteine-tryptophan domain-containing zinc finger
FT protein 3"
FT /id="PRO_0000450711"
FT ZN_FING 21..74
FT /note="CW-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT REGION 131..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 537..651
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..167
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..233
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..435
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 543..585
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..600
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..629
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 630..648
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 33
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 54
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 66
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT MUTAGEN 27
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
FT MUTAGEN 36
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
FT MUTAGEN 52
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
SQ SEQUENCE 902 AA; 99539 MW; 7087E83C790849F4 CRC64;
MPSNGGIIPG PANAASLPAP VLIEDNWVCC DMCHKWRLLP YGTNTSMLPK KWICSMLDWL
PGMNKCDISE DETTNALNAL YVTQIPAAGV SSGGPHTAHA SVAASSTYNI SGQLGQSRKR
KNALKDENCY EHDQQAPAKM TLTSNQQAPA KNREVVDSEH YTNDRDPVST HDLVPQSKSA
SERHKSKHKS RSSHSDGGDL TEKSKKHSKS KNRRGIDRDE HKTSKKTKKE DRHYFNKDWK
NEYDLAGNKV RDETKALSAK AKMSKDSCEQ DEFSLRKEKA SRFDILEKTK RINDDDVAFH
EKMKEHRAGI ETLDLSGKKK TVKEWEDNRL SSMDHTSKGG DNENLNERLS KIKKSEARPE
EVQDANALFS SAGRRQDNEL VADNKFVTCK EGPSELWDNQ PPRQVLNLAE PTRRDVACLQ
SSTVATSSSS KVSSSRRNKN SREAKGSPVE SVSSSPLKNS NTDKISKARK TGKDGELNAD
SSILHTPMKY PTHEVGLLHT GQQAVGEAIL RGSTNNSGMG RVDNQLYPGD KKILDMHGPT
LQPDQQDCFN PRATADSTGH KSKNSAPSRQ GRNGSSNLIS EGNKQIEMSS RKEKLRPSID
NQDMQKSIGQ DNHSHMKEGK SEVHTTRVKP GASKNHTQLR SNVENGDSAS PIRRDGNMIA
FALKEARDLK HKANHLKEKG LELESMGLYF EAALKFLHVA SLWETPNLDN SRSGDVAQSM
KMYSETAKLC SFCAHAYERC NKMASAALAY KCVEVAYLKA AYYKHPSASK DRQELQSVVQ
IAPGESPSSS ASDIDNLNSH GLSKALSTKG GNSPQVAGNH LPLAVRNQAH LLRLLAYTND
VNCAFDATRK SQVAIASAAS SQERGKTVDD GLASVRTVLD FNFNNVNELL RLVRLSMELI
NT
