CWZF5_ORYSJ
ID CWZF5_ORYSJ Reviewed; 1510 AA.
AC Q0DIQ5; A0A0P0WLF5;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Cysteine-tryptophan domain-containing zinc finger protein 5 {ECO:0000303|PubMed:28818372};
DE Short=OsCW-ZF5 {ECO:0000303|PubMed:28818372};
GN Name=CWZF5 {ECO:0000303|PubMed:28818372};
GN OrderedLocusNames=Os05g0371100 {ECO:0000312|EMBL:BAF17268.1},
GN LOC_Os05g30790 {ECO:0000305};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN, AND MUTAGENESIS
RP OF TRP-651; TRP-660 AND TRP-676.
RX PubMed=28818372; DOI=10.1016/j.plantsci.2017.06.013;
RA Zhang Z., Zhang F., Cheng Z.J., Liu L.L., Lin Q.B., Wu F.Q., Zhang H.,
RA Wang J.L., Wang J., Guo X.P., Zhang X., Lei C.L., Zhao Z.C., Zhu S.S.,
RA Wan J.M.;
RT "Functional characterization of rice CW-domain containing zinc finger
RT proteins involved in histone recognition.";
RL Plant Sci. 263:168-176(2017).
CC -!- FUNCTION: Binds to histones H3K4me1, H3K4me2 and H3K4me3 in GST pull-
CC down assay (PubMed:28818372). May facilitate the recruitment of
CC effectors to mediate gene expression (By similarity).
CC {ECO:0000250|UniProtKB:A0A0P0X9Z7, ECO:0000269|PubMed:28818372}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28818372}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young panicles
CC (PubMed:28818372). Expressed at low levels in leaf sheaths, nodes,
CC internodes and axillary buds (PubMed:28818372).
CC {ECO:0000269|PubMed:28818372}.
CC -!- DOMAIN: The CW-type zinc finger domain is not required for nuclear
CC localization. {ECO:0000269|PubMed:28818372}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAS93685.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP008211; BAF17268.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93685.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; Q0DIQ5; -.
DR SMR; Q0DIQ5; -.
DR STRING; 39947.A0A0P0WLF5; -.
DR EnsemblPlants; Os05t0371100-00; Os05t0371100-00; Os05g0371100.
DR Gramene; Os05t0371100-00; Os05t0371100-00; Os05g0371100.
DR eggNOG; ENOG502QS65; Eukaryota.
DR HOGENOM; CLU_1780541_0_0_1; -.
DR InParanoid; Q0DIQ5; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR011124; Znf_CW.
DR Pfam; PF07496; zf-CW; 1.
DR PROSITE; PS51050; ZF_CW; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1510
FT /note="Cysteine-tryptophan domain-containing zinc finger
FT protein 5"
FT /id="PRO_0000450712"
FT ZN_FING 645..698
FT /note="CW-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT REGION 174..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 449..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 582..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 768..893
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1003..1050
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1149..1194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..196
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..464
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 465..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..599
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 799..822
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 832..847
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 873..893
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1003..1017
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1164..1186
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 654
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 657
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 678
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 690
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT MUTAGEN 651
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
FT MUTAGEN 660
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
FT MUTAGEN 676
FT /note="W->A: Abolishes binding to histone H3K4me1/2/3."
FT /evidence="ECO:0000269|PubMed:28818372"
SQ SEQUENCE 1510 AA; 165068 MW; B0B8B39AFCE78CAC CRC64;
MGGGRRGGRR EAEEGVAVAG RSRGVAAAAA RHEVAGELEL ASAGGLGGGG GDELVDPDQL
TYIHNVFKID LYSHVLVNLL VTICKDEKLQ NILGHFQKEF EGGVSAENLG SQYGGYGSFL
PTYQRSPPAL SQSGSPAVLP NHGSASRSPY IPLESVQKNH FVKQAIDGRR KNNYCQRTSS
ENDSNHSQQL LNSGPEQKTA KIRIKVNNKC LERNNAAIYS GLGLDISPSS SIDDSPQWSI
EAPESKLFPD ESADTIFQIM TCHSVPGGLL LSPLAENVLE LRQKSTAVTK KHEAPVYDND
KEELQRNCCH TSSAAPDNNY QLVKKIKLDE QRDHLPEFEN SKYRHKNATI MKKGAKPELK
DISDEIDSIR APRCAKTEKH AVGESADFIA DTSGRLKEAK NGQFKGKGST QSSLSIIDVK
AANSANDDKH PKGKAKLKVT LVRNAKMESS LDDGFSHKTK SDKCNDQPVT TSSQLQIDPA
KKTSLKRDRG KVVCAKDEPS QYKSKELRSL VDAESMGTTT ENVAGNSSEL LKGKKVSALQ
ASLFGKKLKI KTHKKPNYDT TRKPNGENEG YVLDHRNGST YLHTEDKSLK TEKESATSGL
TDKDFSGGGN DGDHKISPIV VDKSASMPSR CKNETTEASM AVPASEPVDQ WVCCDKCETW
RLLPYGMNSD TLPKKWRCSM QSWLPGMNNC KLSEGETTNA IRALYVVPIP ENNISLDSRC
DTATLVRSND AAIMSDNLGM PEISKSSKKL HAPRNRDGLD CFPKLKEKQK RIESSDKGEK
STVTISSGQT MAKDRMHRKR KTSGADYDNL IASKKLKKVY NEPPKHQPPQ FELSKSRPST
KGSLKELPKH TNISPGMGKH ALPSSGKQFC DGDNSDRGAR ASDAGKSDPR DLFIKKNKSK
QMQLRQHGPD PRPSDAFAKH VVKEVLSESN AAKEKLGSDL KFLKVDDHEK SAHARGPVTG
TNSNAIFSEK EDLIEQHLEN IHFQHPLLSE SSVRRNICNV QASTAATSSS SKVSSSHKNK
PEFQETRTSP VESVSSSPLR TSDKKHLDRH RTNSYAVAEI VHSQESVKTG ASCSKEKYGF
ECGSDHTKPH VSGCSNRVMH QDALEDGDLD KQNILTNGVF NNRSSGLGIR NDQGQPNSLV
EQKVNSHVLP IHGSGDFRRP TPDQNGKTLP QYNSNQSDQA KLSSGKHPTQ VRPDKGNVEY
IDLKTNPSTV AGSKLLPGLN NKVNGNASNK SKQSVVENMK HAAIHVDAST PINASALLKE
ARDLKHLSDR LKGKGDDLES ANICFEACLK FLHVASLKEA AGVDSSKQGD PINTMTLYSD
TGNLCGFCAR EFERLKKMAN AALAYKCVEV AYMKAAFYKH PGAIKDSHAL QAASVIAPPA
ESPSSSASDV DNLNNPSTIA KIVSTRGLCT SQIAKNPISR SNHHLMGLLA YSFSYDPFCL
LDKVEDTNYA FEGTRKSQSA FFSYLSGIEK DQADGIALLT EVLNFSFHNV KGLLQLIRHS
LECINHERFK
