ID   CX2_CONMR               Reviewed;          94 AA.
AC   P69770; C8BLS2;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 2.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Conotoxin Gla-MrII;
DE   AltName: Full=Conotoxin Mr12.5;
DE   Flags: Precursor;
OS   Conus marmoreus (Marble cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Conus.
OX   NCBI_TaxID=42752;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=20732855; DOI=10.1093/abbs/gmq066;
RA   Liu Z., Yu Z., Liu N., Zhao C., Hu J., Dai Q.;
RT   "cDNA cloning of conotoxins with framework XII from several Conus
RT   species.";
RL   Acta Biochim. Biophys. Sin. 42:656-661(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 26-75, GAMMA-CARBOXYGLUTAMATION AT GLU-30; GLU-34;
RP   GLU-37; GLU-40 AND GLU-41, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=15194478; DOI=10.1016/j.bbrc.2004.05.088;
RA   Hansson K., Furie B., Furie B.C., Stenflo J.;
RT   "Isolation and characterization of three novel Gla-containing Conus
RT   marmoreus venom peptides, one with a novel cysteine pattern.";
RL   Biochem. Biophys. Res. Commun. 319:1081-1087(2004).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The cysteine framework is XII (C-C-C-C-CC-C-C).
CC   -!- PTM: Contains 4 disulfide bonds.
CC   -!- MASS SPECTROMETRY: Mass=5860.23; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:15194478};
CC   -!- SIMILARITY: Belongs to the conotoxin I2 superfamily. {ECO:0000305}.
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DR   EMBL; GQ228841; ACV13220.1; -; mRNA.
DR   AlphaFoldDB; P69770; -.
DR   TCDB; 8.B.21.1.10; the spider insecticidal neurotoxin cyrtautoxin (cyrautoxin) family.
DR   ConoServer; 1524; Gla-MrII.
DR   ConoServer; 3859; Gla-MrII precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Gamma-carboxyglutamic acid;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   CHAIN           26..75
FT                   /note="Conotoxin Gla-MrII"
FT                   /id="PRO_0000086877"
FT   PROPEP          78..94
FT                   /id="PRO_0000425740"
FT   MOD_RES         30
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   MOD_RES         34
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   MOD_RES         37
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   MOD_RES         40
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   MOD_RES         41
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
SQ   SEQUENCE   94 AA;  10641 MW;  E3891D3505201917 CRC64;
     MFGHTSVSFL LLSIVALGMV ATVICSCDSE FSSEFCEQPE ERICSCSTHV CCHLSSSKRD
     QCMTWNRCLS AQTGNRRSTH MQKRFLRMPR DLAD