ID   CX64_CONAA              Reviewed;          65 AA.
AC   A0A3G3C7T9;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Conotoxin Am6.4 {ECO:0000305};
DE   Flags: Precursor; Fragment;
OS   Conus amadis (Amadis cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Leptoconus.
OX   NCBI_TaxID=198732;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-65, SUBCELLULAR
RP   LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=30593932; DOI=10.1016/j.jprot.2018.12.028;
RA   Vijayasarathy M., Balaram P.;
RT   "Cone snail prolyl-4-hydroxylase alpha-subunit sequences derived from
RT   transcriptomic data and mass spectrometric analysis of variable proline
RT   hydroxylation in C. amadis venom.";
RL   J. Proteomics 194:37-48(2019).
CC   -!- FUNCTION: Probable toxin that inhibits ion channels. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Is not hydroxylated. {ECO:0000269|PubMed:30593932}.
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DR   EMBL; MH282824; AYP73031.1; -; mRNA.
DR   SMR; A0A3G3C7T9; -.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Toxin.
FT   PROPEP          <1..36
FT                   /evidence="ECO:0000305|PubMed:30593932"
FT                   /id="PRO_0000453600"
FT   PEPTIDE         37..65
FT                   /note="Conotoxin Am6.4"
FT                   /id="PRO_0000453601"
FT   REGION          1..33
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..33
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        40..50
FT                   /evidence="ECO:0000305"
FT   DISULFID        45..59
FT                   /evidence="ECO:0000305"
FT   DISULFID        49..64
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT                   /evidence="ECO:0000312|EMBL:AYP73031.1"
SQ   SEQUENCE   65 AA;  7146 MW;  71460ADA6FB0A140 CRC64;
     STGKRNAGKL TVTDDVEADR DTDPDDKDPS VHNSWRTVDC GGVPCEFGCC RIIDGKEKCR
     EIDCD